Publications by authors named "Hanzhong Deng"
Article Synopsis
- - Chiral epichlorohydrin (ECH) is an important intermediate for making chiral pharmaceuticals, but its synthesis faces challenges due to low optical purity caused by reverse reactions.
- - This study developed a new strategy involving an engineered enzyme called HheC (mutant E85P) to improve the synthesis of (S)-ECH by slowing down the reverse reactions while maintaining forward reaction efficiency.
- - The result of this engineering was a significant increase in yield of (S)-ECH to 55.35% and an improvement in optical purity from 92.54% to over 99%, enhancing the potential for green manufacturing of chiral compounds.
The KBr pellet press method for detecting the infrared spectrum of coal is one of the commonly used methods for analyzing the types and content of functional groups in coal. However, KBr crystalline water or moisture has a significant impact on the peak position, peak shape, and peak area of the organic O-H based stretching vibration wave in coal. In this paper, the theoretical characteristics of infrared spectra of phenols and alcohols have been simulated and analyzed using the Gaussian 16 series of programs.
View Article and Find Full Text PDFConformational dynamics is important for enzyme catalysis. However, engineering dynamics to achieve a higher catalytic efficiency is still challenging. In this work, we develop a new strategy to improve the activity of yeast cytosine deaminase (yCD) by engineering its conformational dynamics.
View Article and Find Full Text PDFAsymmetric synthesis of chiral epichlorohydrin (ECH) from 1,3-dichloro-2-propanol (1,3-DCP) using halohydrin dehalogenases (HHDHs) is of great value due to the 100% theoretical yield and high enantioselectivity. The vital problem in the asymmetric synthesis is to prepare optically pure ECH. In this study, key amino acid residues located at halide ion channels of HheC (P175S/W249P) (HheC) were modified to regulate the kinetic parameters.
View Article and Find Full Text PDFAsymmetric synthesis of chiral epichlorohydrin (ECH) from 1,3-dichloro-2-propanol (1,3-DCP) using halohydrin dehalogenase (HHDH) is of great value due to the 100% theoretical yield and high enantioselectivity. In this study, HheC (P175S/W249P) was immobilized on an A502Ps resin and used for the preparation of (S)-ECH. In aqueous system, the immobilized HheC catalyzed the biosynthesis of (S)-ECH with 83.
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