DOT-ELISA AS A FIELD TEST FOR HYDATID DIAGNOSIS.

Cystic hydatid disease (Hydatidosis) is one of the most important parasitic zoonoses and remains a public health and economic problem all over the world. Cyst fluid was obtained from hepatic and pulmonary cysts for demonstration of protoscolices and hooklets. Therefore, a standardized and approachable diagnostic tool for the serodiagnosis of CE is still needed.

Immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica paramyosin antigen.

Background: Many immunological techniques have been developed over years using the different Fasciola antigens for diagnosis of parasitic infection and to replace the parasitological techniques, which are time consuming and usually lack sensitivity and reproducibility.

Materials And Methods: In this study, Fasciola gigantica paramyosin (Pmy) antigen was early detected in cattle sera using sandwich enzyme-linked immunosorbent assay (ELISA), to evaluate the Pmy antigen performance in diagnosis. This work was conducted on 135 cattle blood samples, which were classified according to parasitological investigation into, healthy control (30), fascioliasis (75), and other parasites (30) groups.