Effect of Filler Wire Composition on Weld Metal Microstructure and Mechanical Properties in X80 Steel Laser Welds.

Laser welding was performed using different filler wires, ER70S steel, commercially pure iron, and pure nickel filler, in the context of welding X80 pipeline steel to assess the microstructure and mechanical properties of the weld metal. Introducing an ER70S wire promoted acicular ferrite formation in the fusion zone, compared to a bainitic microstructure in an autogenous laser weld. The use of pure iron wire was considered as a potential strategy for reducing hardenability, as it led to the dilution of alloying elements in the fusion zone, increasing ferrite content and reducing weld metal hardness to a level compliant with API pipeline standards.

Transcriptome Responses to Different Salinity Conditions in Revealed by Long-Read Sequencing.

The marine nematode is widely distributed in intertidal zones around the globe, yet the mechanisms underlying its broad adaptation to salinity remain elusive. In this study, we applied ONT long-read sequencing technology to unravel the transcriptome responses to different salinity conditions in . Through ONT sequencing under 3‱, 30‱ and 60‱ salinity environments, we obtained 131.

Secretory leukocyte protease inhibitor as a novel predictive biomarker in patients with diabetic kidney disease.

Background: Secretory leukocyte protease inhibitor (SLPI) is a multifunctional protein involved in the chronic inflammatory process, implicated in the pathogenesis of diabetic kidney disease (DKD). However, its potential as a diagnostic and prognostic biomarker of DKD has yet to be evaluated. This study explored the clinical utility of SLPI in the diagnosis and prognosis of renal endpoint events in patients with DKD.

A Printable Magnetic-Responsive Iron Oxide Nanoparticle (ION)-Gelatin Methacryloyl (GelMA) Ink for Soft Bioactuator/Robot Applications.

The features or actuation behaviors of nature's creatures provide concepts for the development of biomimetic soft bioactuators/robots with stimuli-responsive capabilities, design convenience, and environmental adaptivity in various fields. is a mechanically responsive plant that can convert pressure to the motion of leaves. When the leaves receive pressure, the occurrence of asymmetric turgor in the extensor and flexor sides of the pulvinus from redistributing the water in the pulvinus causes the bending of the pulvinus.

Photo-crosslinked bioactive BG/BMSCs@GelMA hydrogels for bone-defect repairs.

The clinical treatments of bone defects remain a challenge. Hydrogels containing bone marrow mesenchymal stem cells (BMSCs) are extensively used to bone regeneration because of excellent biocompatibility and hydrophilicity. However, the insufficient osteo-induction capacity of the BMSC-loaded hydrogels limits their clinical applications.

Simple Self-Powered Sensor for the Detection of DO and Other Isotopologues of Liquid Water.

Distinguishing between heavy water and regular water has been a continuing challenge since these isotopologues of water have very similar physical and chemical properties. We report the development and evaluation of a simple, inexpensive sensor capable of detecting liquid DO and other isotopologues of liquid water through the measurement of electrical signals generated from a nanoporous alumina film. This electrical output, consisting of a sharp voltage pulse followed by a separate broad voltage pulse, is present during the application of microliter volumes of liquid.

Combined addition of L-carnitine and L-proline improves cryopreservation of dairy goat semen.

Cryopreservation of semen renders artificial insemination easier and cheaper compared to use of fresh semen. However, the cellular oxidative stress, toxicity of cryoprotectants, and osmotic imbalance may lead to a decline in semen quality and fertilization ability during the process of cryopreservation. L-carnitine and L-proline have been demonstrated to possess effective antioxidant properties in cryopreservation, with the latter also exhibiting excellent permeability and thus being utilized as a permeable cryoprotectant in the field.

Elucidating osseointegration in vivo in 3D printed scaffolds eliciting different foreign body responses.

Osseointegration between biomaterial and bone is critical for the clinical success of many orthopaedic and dental implants. However, the mechanisms of in vivo interfacial bonding formation and the role of immune cells in this process remain unclear. In this study, we investigated the bone-scaffold material interfaces in two different 3D printed porous scaffolds (polymer/hydroxyapatite and sintered hydroxyapatite) that elicited different levels of foreign body response (FBR).

A long non-coding RNA essential for early embryonic development improves somatic cell nuclear transfer somatic cell nuclear transfer efficiency in goats.

In Brief: Early embryonic development in goats is a complex and an important process. This study identified a novel long non-coding RNA (lncRNA), lncRNA3720, that appears to affect early embryonic development in goats through histone variants.

Abstract: Although abundant lncRNAs have been found to be highly expressed in early embryos, the functions and mechanisms of most lncRNAs in regulating embryonic development remain unclear.

EAT-2 attenuates C. elegans development via metabolic remodeling in a chemically defined food environment.

Dietary intake and nutrient composition regulate animal growth and development; however, the underlying mechanisms remain elusive. Our previous study has shown that either the mammalian deafness homolog gene tmc-1 or its downstream acetylcholine receptor gene eat-2 attenuates Caenorhabditis elegans development in a chemically defined food CeMM (C. elegans maintenance medium) environment, but the underpinning mechanisms are not well-understood.

Immunogenicity Analysis of PCV3 Recombinant Capsid Protein Virus-like Particles and Their Application in Antibodies Detection.

Porcine circovirus type 3 is a newly emerging pathogen of porcine circovirus associated disease (PCVAD). Currently, there is no commercially available vaccine, resulting in huge economic losses to the pig industry. Porcine circovirus type 3 capsid protein (Cap) can self-assemble into virus-like particles (VLPs).

Bionic Structure Inspired by Tree Frogs to Enhance Damping Performance.

Magnetic fluid shock absorbers (MFSAs) have been successfully utilized to eliminate microvibrations of flexible spacecraft structures. The method of enhancing the damping efficiency of MFSAs has always been a critical issue. To address this, we drew inspiration from the tree frog's toe pads, which exhibit strong friction due to their unique surface structure.

The research development of STAT3 in hepatic ischemia-reperfusion injury.

Ischemia-reperfusion injury (IRI) is a common complication of surgery, which can cause rapid deterioration of the liver function, increase the risk of graft rejection, and seriously affect the prognosis of patients. The signal transducer and activator of transcription 3 (STAT3) protein has been implicated in pathogenesis of IRI. STAT3 influences the mitochondria through multiple pathways and is also involved in apoptosis and other forms of programmed cell death.

Preliminary mechanism of inhibitor of SGLT2 in fatty liver cold ischemia injury.

With rapid development of liver transplantation technology, the demand for transplants have reached beyond the supply of organs, and thus development of effective strategies to reduce cold ischemia injury in fatty liver is important. Here, we explored the potential effect of SGLT-2 inhibitor in cold ischemia injury, fatty livers from 2 weeks methionine and choline deficient diet (MCD) rats were administered. After one week of intragastric administration of Sodium-dependent glucose transporters (SGLT-2) inhibitor empagliflozin (EMPA) or NaCI, liver were stored for 24 h.

Loss of linker histone H1 in the maternal genome influences DEMETER-mediated demethylation and affects the endosperm DNA methylation landscape.

The DEMETER (DME) DNA glycosylase demethylates the central cell genome prior to fertilization. This epigenetic reconfiguration of the female gamete companion cell establishes gene imprinting in the endosperm and is essential for seed viability. DME demethylates small and genic-flanking transposons as well as intergenic and heterochromatin sequences, but how DME is recruited to these loci remains unknown.

Qing-Re-Xiao-Zheng-Yi-Qi formula relieves kidney damage and activates mitophagy in diabetic kidney disease.

Qing-Re-Xiao-Zheng-Yi-Qi Formula is an effective prescription in diabetic kidney disease treatment, we have confirmed the efficacy of Qing-Re-Xiao-Zheng therapy in diabetic kidney disease through clinical trials. In this study, we investigated the mechanisms of Qing-Re-Xiao-Zheng-Yi-Qi Formula in the treatment of diabetic kidney disease. We used Vanquish UHPLC to analyze the chemical profiling of Qing-Re-Xiao-Zheng-Yi-Qi Formula freeze-dried powder.

ZnO nanoparticle modified chitosan/borosilicate bioglass composite scaffold for inhibiting bacterial infection and promoting bone regeneration.

The treatment of implant-associated bone infection remains a significant clinical challenge. However, bone scaffolds with antimicrobial activity and osteoinductive properties can prevent these infections and improve clinical outcomes. In this study, borosilicate bioglass and chitosan composite scaffolds were prepared, and then the surface was modified with nano-zinc oxide.

Upgradation of sludge deep dewatering conditioners through persulfate activated by ferrous: Compatibility with sludge incineration, dewatering mechanism, ecological risks elimination and carbon emission performance.

Serious loss of organic substances and notable release of refractory intracellular organics and cell-free antibiotic resistance genes (ARGs) caused by cell lysis are found when quick lime, FeCl, and cationic polyacrylamide (CPAM) were used as sludge conditioners, which is not feasible to sludge separate incineration and increases ecological risks. Therefore, persulfate oxidation through ferrous (Fe-NaSO) activation was applied for the upgradation of sludge conditioner in China, the specific resistance to filtration (SRF) and capillary suction time (CST) significantly decreased and the removed water increased from 40% to 54%, implying that the persulfate activated by ferrous (PAF) conditioner presents good performance in sludge dewatering. Organic matter content and heating value of sludge merely decreased, and Cl content in sludge simultaneously decreased with the use of the PAF conditioner, thereby effectively reducing the corrosion risk to the incinerator and showing good compatibility with sludge separate incineration.

Factors associated with using the internet for medical information based on the doctor-patient trust model: a cross-sectional study.

Background: Internet medical care has been advancing steadily, especially during the coronavirus disease 2019 pandemic, the development momentum of Internet medical care in China is more vigorous. This study aimed to explore the factors associated with using the Internet for medical information, to examine the popularisation and implementation of Internet medical treatment and feasible strategies, and promote the further development of Internet medical treatment.

Methods: A cross-sectional study was conducted on 408 medical patients who had used online medical services.

PMS activation by magnetic cobalt-N-doped carbon composite for ultra-efficient degradation of refractory organic pollutant: Mechanisms and identification of intermediates.

Refractory organic pollutant effluent has led to severe water pollution. In this study, magnetic Co-N-doped carbon hybrid catalysts (Co-NC-x) were fabricated using a facile cation exchange combined pyrolysis and self-reduction technique to activate peroxymonosulfate (PMS) for rehabilitation of the water environment. Factors affecting the catalytic activity of the Co-NC-850 were comprehensively examined.

Respiratory Motion Mitigation and Repeatability of Two Diffusion-Weighted MRI Methods Applied to a Murine Model of Spontaneous Pancreatic Cancer.

Respiratory motion and increased susceptibility effects at high magnetic fields pose challenges for quantitative diffusion-weighted MRI (DWI) of a mouse abdomen on preclinical MRI systems. We demonstrate the first application of radial k-space-sampled (RAD) DWI of a mouse abdomen using a genetically engineered mouse model of pancreatic ductal adenocarcinoma (PDAC) on a 4.7 T preclinical scanner equipped with moderate gradient capability.

Transcriptome Analysis of the Nematode in Acidic Stress Environments.

Ocean acidification and acid rain, caused by modern industries' fossil fuel burning, lead to a decrease in the living environmental pH, which results in a series of negative effects on many organisms. However, the underlying mechanisms of animals' response to acidic pH stress are largely unknown. In this study, we used the nematode as an animal model to explore the regulatory mechanisms of organisms' response to pH decline.

Magnetic FeO-N-doped carbon sphere composite for tetracycline degradation by enhancing catalytic activity for peroxymonosulfate: A dominant non-radical mechanism.

The design of sustainable, effective and recyclable hybrid catalysts for advanced oxidation processes is highly significant for remediation of the water environment. In this study, we synthesized magnetic FeO-N-doped carbon sphere composite catalysts (FeO-NCS-x) for efficient removal of tetracycline by activating peroxymonosulfate (PMS). The FeO-NCS-x composite was obtained by facile hydrothermal treatment of chitosan-iron complexes followed by pyrolysis.