Potential interaction of sugar intake and tobacco exposure on dental caries in adults-A cross-sectional study from the National Health and Nutrition Examination Survey.

Background/purpose: We suspected that there might be an interaction between sugar intake and tobacco exposure on the risk of dental caries. The study aimed to investigate the associations of sugar intake or tobacco exposure with the risk of dental caries.

Materials And Methods: This cross-sectional study obtained data of 18804 participants from National Health and Nutrition Examination Survey (NHANES) between 2011 and 2018.

Maxillary second molar with four roots and five canals.

In this case report, we present a maxillary second molar variant, which had two palatal roots with two canals and two buccal roots with three canals, including a second mesiobuccal canal. A 44-year-old female patient complained about a tooth crown fracture and severe pain in her right maxillary second molar. A clinical intraoral inspection and radiography were carried out on the tooth, and a diagnosis of chronic apical periodontitis was made.

Hey1 functions as a positive regulator of odontogenic differentiation in odontoblast‑lineage cells.

Substantial evidence has indicated that Notch and bone morphogenetic protein (BMP) signaling may regulate odontoblastic differentiation. Hairy/enhancer‑of‑split related with YRPW motif 1 (Hey1), a downstream target gene of Notch and BMP signaling, is expressed in dental pulp tissues and has been demonstrated to be responsible for osteoblast mineralization. The aim of this study was to investigate the effects of Hey1 on odontoblast differentiation.

Dental education evaluation in China: a systematic review.

Background: Accreditation of education is very important for maintaining and improving education quality. With the development of modern dental education, more and more attention is being paid to accreditation of dental education in China. Current accreditation of dental education in China is called "dental education evaluation".

A brief comparison of curricula at dental schools in China and Japan.

This study compared the dental curricula at dental schools in China and Japan. A survey was conducted in representative dental schools in China and Japan. It was found that, in China and Japan where dental schools recruit students directly from high schools, more attention is paid to introduction of early professional education.

Detection of vertical root fractures in non-endodontically treated molars using cone-beam computed tomography: a report of four representative cases.

Vertical root fractures (VRFs) in teeth without endodontic treatment are relatively uncommon. The diagnosis is based on clinical and radiographic presentations. It might be difficult to detect VRFs in non-endodontically treated molars by conventional radiographs in certain situations because of the limitations of 2D images and many others factors such as the VRFs being in their early stages.

Evaluation of horizontal/oblique root fractures in the palatal roots of maxillary first molars using cone-beam computed tomography: a report of three cases.

Diagnosis of root fractures is generally based on clinical and radiographic presentations. This case report aims to detect horizontal/oblique root fractures in posterior teeth using a combination of conventional radiographs and cone-beam computed tomography (CBCT). Three maxillary first molars with horizontal/oblique root fractures in the palatal roots are presented.

Horizontal root fractures in posterior teeth: a case series.

This case series describes observations of 12 patients who developed horizontal root fractures in non-endodontically treated teeth. Using clinical and radiographic examination, horizontal fractures were observed in eight maxillary first molars, one maxillary second molar, one mandibular first premolar and four mandibular second premolars. A total of 12 teeth had clinically intact crowns and 2 had carious.

Expression of Notch signalling-related genes in normal and differentiating rat dental pulp cells.

Notch signalling is of fundamental importance to various processes during embryonic development and in adults. The possible role of Hey1, an important Notch signalling component, in odontoblast differentiation was evaluated in this study. Primary cultured dental pulp cells, derived from upper incisors of 5-week-old Wistar rats, were placed in alpha-modification of Eagle's minimal essential medium supplemented with 10% Fetal Bovine Serum (FBS), and ascorbic acid (AA) and beta-glycerophosphate (beta-GP), with or without dexamethasone, and cultured on dishes coated with collagen type IA for 7 days.

[Study of cbfalpha1 on the expression of extracellular matrix proteins in dental papilla cells induced by BMP-2 in vitro].

Purpose: To evaluate the function of cbfalpha1 on BMP-2 signaling to extracellular matrix proteins in dental papilla cells in vitro.

Methods: RT-PCR and Western blot were performed to detect the expression of ALP, OC, ON, OPN, BSP, DMP-1 and DSPP in cultured dental papilla cells induced by 200ng/mL BMP-2 and/or down-regulated by cbfalpha1 antisense technology, the results were analysed with SPSS 11.0 software package.

[Generation of transgenic mouse of dentin sialoprotein and transgene expression analysis].

Purpose: To generate the transgenic mouse model of DSP and perform transgene expression analysis by RT-PCR.

Methods: Plasmid pcDNA3.1-CX was constructed by substituting promoter cbeta-actin for CMV promoter of pcDNA3.

[Generation of dspp-LacZ transgenic mouse founders].

Purpose: To establish a transgenic mouse founders in which the expression of LacZ was directed by a dentin sialophosphoprotein-specific promoter.

Methods: The DSPP-specific promoter was obtained by PCR and confirmed by sequencing, and the transgenic plasmid, pTN-DPM-LacZ, was constructed by subcloning the DSPP-specific promoter and LacZ-encoding sequence into one vector. The linearized transgenic plasmid was microinjected into the male pronucleus of the zygotes, and the microinjected zygotes were implanted to recipient pseudopregnant mice.