Publications by authors named "Hans Wijaya"
Optimized expression of large fragment DNA polymerase I from in expression system.
Prep Biochem Biotechnol
April 2023
DNA polymerase is a DNA polymerase derived from has a strand-displacement activity, and is used in loop-mediated isothermal amplification (LAMP) for rapid detection of COVID-19. Despite its potential to be employed in the detection of COVID-19, using commercially available enzymes is not economically feasible. The use of noncommercial enzyme for routine use is desirable.
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- - The study developed a budget-friendly method for producing bioethanol using a specific yeast strain (S. cerevisiae F118) and concentrated sweet sorghum juice, achieving a higher ethanol yield than a standard strain.
- - The F118 strain achieved an impressive ethanol titer of 86.19 g/L, outperforming the non-flocculent BY4741 strain, which produced only 33.92 g/L, due to better gene expression linked to sugar processing.
- - After multiple fermentation cycles, the F118 strain demonstrated successful separation from the liquid through floc formation, resulting in an ethanol concentration of 100.37 g/L by the fifth cycle.