Quantitative imaging through a spectrograph. 1. Principles and theory.

Laser-based optical diagnostics, such as planar laser-induced fluorescence and, especially, Raman imaging, often require selective spectral filtering. We advocate the use of an imaging spectrograph with a broad entrance slit as a spectral filter for two-dimensional imaging. A spectrograph in this mode of operation produces output that is a convolution of the spatial and spectral information that is present in the incident light.

Quantitative evaluation of experimental NMR restraints.

Nuclear Overhauser effect (NOE) data are an indispensable source of structural information in biomolecular structure determination by NMR spectroscopy. The number and type of experimental restraints used in the structure calculation and the RMS deviation of the restraints are usually reported. We present a new method for quantifying the information contained in the experimental NMR restraints.