Differentiation potential of mesenchymal progenitor cells following transplantation into calvarial defects.

The complexity of stem cell lineage commitment requires studies to investigate the intrinsic and extrinsic regulatory events during differentiation. The objective of this long-term in vivo study was to investigate cellular differentiation and tissue formation of transplanted undifferentiated bone-marrow-derived mesenchymal progenitor cells (BMPCs) in combination with a medical grade polycaprolactone (mPCL) scaffold and to compare them to osteoblasts; a more differentiated cell type in a calvarial defect model. Tissue formation was assessed via histology, mechanical and radiological methods after 3 12, and 24 months.

Engineering of vascularized adipose constructs.

Adipose tissue engineering offers a promising alternative to the current surgical techniques for the treatment of soft tissue defects. It is a challenge to find the appropriate scaffold that not only represents a suitable environment for cells but also allows fabrication of customized tissue constructs, particularly in breast surgery. We investigated two different scaffolds for their potential use in adipose tissue regeneration.

[Diagnosis of the deep partial-thickness burn wound of Skh-1 mouse with Optical Coherence Tomography].

Objective: To evaluate the application value of Optical Coherence Tomography (OCT) in the diagnosis of the depth of burn wound.

Methods: Deep partial-thickness scald models of Skh-1 mice were reproduced using self-made steam scald appliance. The scald wounds were scanned with OCT 3 hours, or 3 and 8 days after injury respectively.