Substrate diversity of NSUN enzymes and links of 5-methylcytosine to mRNA translation and turnover.

Maps of the RNA modification 5-methylcytosine (mC) often diverge markedly not only because of differences in detection methods, data depand analysis pipelines but also biological factors. We re-analysed bisulfite RNA sequencing datasets from five human cell lines and seven tissues using a coherent mC site calling pipeline. With the resulting union list of 6,393 mC sites, we studied site distribution, enzymology, interaction with RNA-binding proteins and molecular function.