FERONIA-mediated TIR1/AFB2 oxidation stimulates auxin signaling in Arabidopsis.

The phytohormone auxin plays a pivotal role in governing plant growth and development. Although the TRANSPORT INHIBITOR RESPONSE1/AUXIN SIGNALING F-BOX (TIR1/AFB) receptors function in both the nucleus and cytoplasm, the mechanism governing the distribution of TIR1/AFBs between these cellular compartments remains unknown. In this study, we demonstrate that auxin-mediated oxidation of TIR1/AFB2 is essential for their targeting to the nucleus.

Decoding plant adaptation: deubiquitinating enzymes UBP12 and UBP13 in hormone signaling, light response, and developmental processes.

Ubiquitination, a vital post-translational modification in plants, plays a significant role in regulating protein activity, localization, and stability. This process occurs through a complex enzyme cascade that involves E1, E2, and E3 enzymes, leading to the covalent attachment of ubiquitin molecules to substrate proteins. Conversely, deubiquitinating enzymes (DUBs) work in opposition to this process by removing ubiquitin moieties.

Integrative Proteome and Phosphoproteome Profiling of Early Cold Response in Maize Seedlings.

Cold limits the growth and yield of maize in temperate regions, but the molecular mechanism of cold adaptation remains largely unexplored in maize. To identify early molecular events during cold shock, maize seedlings were treated under 4 °C for 30 min and 2 h, and analyzed at both the proteome and phosphoproteome levels. Over 8500 proteins and 19,300 phosphopeptides were quantified.

STOP1 Regulates Transcription and Coordinates K/NH Balance in Response to Low-K Stress.

Potassium and nitrogen are essential mineral elements for plant growth and development. The protein kinase LKS1/CIPK23 is involved in both K and NH uptake in root. The transcripts of can be induced by low K (0.

Data-Independent Acquisition-Based Proteome and Phosphoproteome Profiling Reveals Early Protein Phosphorylation and Dephosphorylation Events in Seedlings upon Cold Exposure.

Protein phosphorylation plays an important role in mediating signal transduction in cold response in plants. To better understand how plants sense and respond to the early temperature drop, we performed data-independent acquisition (DIA) method-based mass spectrometry analysis to profile the proteome and phosphoproteome of seedlings upon cold stress in a time-course manner (10, 30 and 120 min of cold treatments). Our results showed the rapid and extensive changes at the phosphopeptide levels, but not at the protein abundance levels, indicating cold-mediated protein phosphorylation and dephosphorylation events.

ZMK1 Is Involved in K Uptake and Regulated by Protein Kinase ZmCIPK23 in .

Potassium (K) is one of essential mineral elements for plant growth and development. K channels, especially AKT1-like channels, play crucial roles in K uptake in plant roots. Maize is one of important crops; however, the K uptake mechanism in maize is little known.

LORELEI-LIKE GPI-ANCHORED PROTEINS 2/3 Regulate Pollen Tube Growth as Chaperones and Coreceptors for ANXUR/BUPS Receptor Kinases in Arabidopsis.

Pollen tube growth is crucial for successful fertilization. In Arabidopsis thaliana, the ANXUR (ANX)/BUPS receptor kinase complex controls and maintains pollen tube growth in response to autocrine rapid alkalinization factor 4/19 (RALF4/19) signaling; however, the molecular and cellular mechanisms underlying the ANX/BUPS-mediated regulation of pollen tube growth remain unclear. In this study, we found that pollen-specific LORELEI-like GPI-anchored proteins 2 and 3 (LLG2/3) promote pollen tube growth in vitro and in vivo.

Calcineurin B-like protein CBL10 directly interacts with AKT1 and modulates K+ homeostasis in Arabidopsis.

Potassium transporters and channels play crucial roles in K+ uptake and translocation in plant cells. These roles are essential for plant growth and development. AKT1 is an important K+ channel in Arabidopsis roots that is involved in K+ uptake.