Survey report on keratoplasty in China: A 5-year review from 2014 to 2018.

To provide the general information on corneal transplantation (CT) in China, China Cornea Society designed a questionnaire on CT from 2014 to 2018 and entrusted it to 31 committee members for implementation of the survey nationwide. This article presents the results of the survey and compares the indicators used in the survey and those in the annual statistical report released by the Eye Bank Association of America (EBAA). The number of corneal transplantations completed by the 64 hospitals from 2014 to 2018 was respectively 5377, 6394, 7595, 8270 and 8980, totally 36,616 (22,959 male and 13,657 female).

A novel mutation in C5L2 gene was associated with hyperlipidemia and retinitis pigmentosa in a Chinese family.

Background: Previous studies indicated that hyperlipidemia was associated with retinitis pigmentosa (RP). We aimed to identify the mutations in the C5L2 gene which was reported to be associated with hyperlipidemia in a Chinese family with (RP).

Methods: The Proband from the family was screened for mutations in the C5L2 gene that was known to cause hyperlipidemia.

Toxicity and pharmacokinetics of intrastromal injection of amphotericin B in a rabbit model.

Purpose: To investigate the ocular toxicity and pharmacokinetics of intrastromal injection of amphotericin B (AmB) in a rabbit model.

Methods: Forty albino rabbits were randomly divided into five groups (eight per group). The rabbits were anesthetized before they received the medication.

[Effects of tobramycin dexamethasone eye ointment for blepharitis: multi-center clinical trial].

Objective: To realize the effectiveness and security of Tobramycin and Dexamethasone Eye Ointment for blepharitis treatment. Design Case control studies. Participants 148 patients be diagnosed as blepharitis including 81 cases as research group and 67 cases as control group.

Binocular form deprivation influences the visual cortex.

α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors are considered to play a crucial role in synaptic plasticity in the developing visual cortex. In this study, we established a rat model of binocular form deprivation by suturing the rat binocular eyelids before eye-opening at postnatal day 14. During development, the decay time of excitatory postsynaptic currents mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors of normal rats became longer after eye-opening; however, the decay time did not change significantly in binocular form deprivation rats.

[A primary study of L-carnitine protective effect on corneal and conjunctival epithelium of mouse dry eye model induced by hyperosmolar saline].

Objective: To evaluate protective effects of L-carnitine on corneal and conjunctival epithelium of mouse dry eye model induced by hyperosmolar saline.

Methods: Sixty female BALB/c mice at the age of 6 - 8 weeks were randomly divided into three groups (20 in each): Hyperosmolar saline group (HO), Hyperosmolar and Isosmotic saline group (HO + IO), as well as Hyperosmolar saline and 1% L-carnitine group (HO + 1%LCA). The HO group was treated with 500 mOsmol/L sodium chloride solution; the HO + IO group with 308 mOsmol/L sodium chloride solution first and 500 mOsmol/L sodium chloride solution 30 minutes later; and the HO + 1%LCA group with 1%L-carnitine eye drop first and 500 mOsmol/L sodium chloride solution 30 minutes later.

A comparison of cyclosporine 0.05% ophthalmic emulsion versus vehicle in Chinese patients with moderate to severe dry eye disease: an eight-week, multicenter, randomized, double-blind, parallel-group trial.

Purpose: The goals of this study were to compare the efficacy and safety profile of topical cyclosporine 0.05% versus vehicle in Chinese patients with moderate to severe dry eye disease.

Methods: In this multicenter, randomized, double-blind, vehicle-controlled, parallel-group study, 233 dry eye patients were allocated to receive either cyclosporine 0.

Corneal and aqueous humor concentrations of amphotericin B using three different routes of administration in a rabbit model.

Background: Little is known about the ocular penetration following intrastromal or intracameral injection of amphotericin B (AMB), the current drug of choice in fungal keratitis. Concentrations of AMB were investigated in the cornea and aqueous humor of rabbits after using one of three different routes of administration: topical 0.25% AMB eye drops and intrastromal and intracameral injection of AMB (10 microg).

Activation of retinal stem cells in the proliferating marginal region of RCS rats during development of retinitis pigmentosa.

Retinal stem cells (RSCs) have been demonstrated at the proliferating marginal regions from the pars plana of ciliary body to the ciliary marginal zone (CMZ) in adult lower vertebrates and mammals. Investigations in the lower vertebrates have provided some evidence that RSCs can proliferate following retinal damage; however, the evidence that this occurs in mammals is not clear. In this study, we explored RSCs proliferation potential of adult mammalian in proliferating marginal regions of Royal College of Surgeons (RCS) rats, an animal model for retinitis pigmentosa (RP).

Tear film function in type 2 diabetic patients with retinopathy.

Purpose: To investigate whether diabetes mellitus is correlated with tear film dysfunction.

Methods: Tear film function tests including tear film breakup time (BUT), fluorescein staining, Schirmer I test, rose Bengal staining, total tear protein detection, tear sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and TMS-4 corneal topography were performed. A dry-eye questionnaire was used.

Effect of topical FK506 used alone or combined with keratoplasty on patients with recurrent Mooren's corneal ulcer.

Purpose: To evaluate the efficacy of topical 0.1% FK506 used alone or combined with keratoplasty on patients with recurrent Mooren's ulcer.

Methods: This is a retrospective interventional consecutive case series.

[Analysis of current status of eye bank construction in Western China].

Objective: To evaluate the current status of eye bank construction in Western China.

Methods: The numbers of eye banks, equipments of eye banks, methods of donor cornea preservation, fund for managing eye banks, technicians of eye banks, and difficulties in the construction of eye bank were surveyed by questionnaires from ophthalmology associations and eye banks in the following provinces and city: Sichuan province, Yunnan province, Guizhou province, Guangxi Zhuang autonomous region, Tibet autonomous region, Shanxi province, Gansu province, Ningxia Hui autonomous region, Qinghai province, Xinjiang Weiwuer autonomous region, Neimonggu autonomous region, and city of Chongqing before March 2003, and the numbers of eye banks and donor corneas obtained from January 1999 to March 2003 were also included in the study.

Results: The current situation of eye bank construction in Western China showed: (1) There were total 8 eye banks in 6 provinces among the 12 provinces and city of Western China.

Studies on the effects of the immunosuppressant FK-506 on the high-risk corneal graft rejection.

Purpose: To evaluate the clinical efficacy of FK-506 on suppressing high-risk cornea transplantation rejection.

Methods: In a randomized controlled clinical trial, 56 eyes of 56 patients with high-risk keratoplasty (including total corneal transplantation TCT, total corneal transplantation with circular lamellar sclera CST, vascularization corneal transplantation and corneal retransplantation) were divided into the experimental group and the control group (each with 28 eyes). The experimental group was treated by FK-506 eyedrops (0.

Microsurgical treatment of Mooren's corneal ulcer.

This study evaluated the effect of microsurgery of lamellar keratoplasty (LKP) on Mooren's corneal ulcer. The surgical effect, postoperative astigmatism, postoperative vision, postoperative ulcer recurrence, and surgical complications of 2 groups of consecutive Mooren's ulcer inpatients treated respectively by non-microsurgery of LKP and microsurgery of LKP were analyzed. There were significant differences of the effect, postoperative astigmatism, postoperative vision, postoperative ulcer recurrence, and surgical complications between the 2 groups.

[A primary study on soluble protein and soluble antigen of corneal stroma in Mooren ' s ulcer patients].

Objective: To investigate changes of soluble protein and soluble antigen of corneal stroma in Mooren's ulcer patients and autoantibody in the patients' sera against the soluble antigen.

Methods: The corneal stromal specimens taken from 15 Mooren's ulcer patients who received lamellar keratoplasty were studied. Soluble protein and soluble antigen of the corneal stroma were extracted.

[Effect of FK506 eye-drop combined with keratoplasty on recurrent Mooren's ulcer].

Objective: To observe the effect of FK506 eye-drop combined with keratoplasty on recurrent Mooren's ulcer.

Methods: 9 cases (15 eyes) of recurrent Mooren's ulcer were treated with topical FK506 eye-drop combined with keratoplasty. Of the 15 eyes, two of Mooren's ulcer smaller than half circle of the limbus were treated with 0.