Publications by authors named "Hannes G Roggenkamp"
Background: The spread of multi-resistant bacteria endangers the effectiveness of empirical antimicrobial treatment, particularly in Gram-negative bloodstream infections. Thus, rapid and reliable susceptibility testing has become a key challenge of modern microbiology. Here, we evaluated a combination disc test for rapid detection of ESBL production in Escherichia coli (rapid combination disc test, RCDT) directly from blood cultures.
View Article and Find Full Text PDFThe formation of Ca microdomains during T cell activation is initiated by the production of nicotinic acid adenine dinucleotide phosphate (NAADP) from its reduced form NAADPH. The reverse reaction—NAADP to NAADPH—is catalyzed by glucose 6-phosphate dehydrogenase (G6PD). Here, we identified NADPH oxidases NOX and DUOX as NAADP-forming enzymes that convert NAADPH to NAADP under physiological conditions in vitro.
View Article and Find Full Text PDFArticle Synopsis
- NAADP is a molecule that helps T cells start their activation by causing calcium (Ca) to be released in tiny areas called microdomains.
- Scientists found a special protein called HN1L (or JPT2) that helps NAADP work properly in this process.
- When they removed HN1L from certain T cells, it made it harder for them to produce these calcium signals quickly, showing that HN1L plays a crucial role in T cell activation.