Online monitoring of the respiratory quotient reveals metabolic phases during microaerobic 2,3-butanediol production with .

Microaerobic cultivation conditions are often beneficial for the biotechnological production of reduced metabolites like 2,3-butanediol. However, due to oxygen limitation, process monitoring based on oxygen transfer rate, or dissolved oxygen measurement provides only limited information. In this study, online monitoring of the respiratory quotient is used to investigate the metabolic activity of DSM 8785 during mixed acid-2,3-butanediol production under microaerobic conditions.

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production.

Background: Production of 2,3-butanediol from renewable resources is a promising measure to decrease the consumption of fossil resources in the chemical industry. One of the most influential parameters on biotechnological 2,3-butanediol production is the oxygen availability during the cultivation. As 2,3-butanediol is produced under microaerobic process conditions, a well-controlled oxygen supply is the key parameter to control biomass formation and 2,3-butanediol production.

Designer rhamnolipids by reduction of congener diversity: production and characterization.

Background: Rhamnolipids are biosurfactants featuring surface-active properties that render them suitable for a broad range of industrial applications. These properties include their emulsification and foaming capacity, critical micelle concentration, and ability to lower surface tension. Further, aspects like biocompatibility and environmental friendliness are becoming increasingly important.