Background: The mechanism by which highly diluted and agitated solutions have their effect is still unknown, but the development in recent years of new methods identifying changes in water and solute dipole moments is providing insights into potential modes of action.
Objective: The objective of the current study was to compare the biological effects of (AC) previously obtained by our group and already described in the literature with now measurable physico-chemical effects on solvatochromic dyes.
Methods: Different dilutions of AC and succussed water have been characterized with respect to their effect on the visible spectra of the solvatochromic dyes methylene violet (MV), a pyridinium phenolate (ET33), and a dimethylamino naphthalenone (BDN) compared with in-vitro action against -infected macrophages.