Publications by authors named "Hannah K Dewald"
Article Synopsis
- * The study introduces a new ELISA protocol that effectively detects antibodies against the Receptor Binding Domain (RBD) of the virus's Spike protein, showing accuracy and versatility compared to commercial assays.
- * This protocol allows for testing using various sample types, including breast milk and dried blood from remote collection, making it ideal for large-scale studies outside of traditional healthcare settings.
Article Synopsis
- * This study presents an ELISA protocol that detects antibodies to the virus and is both accurate and versatile, performing well compared to established commercial tests.
- * The ELISA can utilize various types of biospecimens, including blood and breast milk, and is suitable for remote sample collection, making it ideal for large-scale studies beyond healthcare environments.
Aging leads to a progressive functional decline of the immune system, rendering the elderly increasingly susceptible to disease and infection. The degree to which immune cell senescence contributes to this decline remains unclear, however, since markers that label immune cells with classical features of cellular senescence accurately and comprehensively have not been identified. Using a second-generation fluorogenic substrate for β-galactosidase and multi-parameter flow cytometry, we demonstrate here that peripheral blood mononuclear cells (PBMCs) isolated from healthy humans increasingly display cells with high senescence-associated β-galactosidase (SA-βGal) activity with advancing donor age.
View Article and Find Full Text PDFPlasmacytoid dendritic cells (pDCs) are innate immune cells and potent producers of interferon alpha (IFNα). Regulation of pDCs is crucial for prevention of aberrant IFN production. Transcription factor E2-2 (TCF4) regulates pDC development and function, but mechanisms of E2-2 control have not been investigated.
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