Lactobacilli Downregulate Transcription Factors in That Affect Motility, Acid Tolerance and Antimicrobial Peptide Survival.

infection triggers inflammation that may lead to gastritis, stomach ulcers and cancer. Probiotic bacteria, such as , have been of interest as treatment options, however, little is known about the molecular mechanisms of -mediated inhibition of pathogenesis. In this work, we investigated the effect of culture supernatants, so-called conditioned medium (CM), from two gastric isolates, and , on the expression of transcriptional regulators in .

Lactate causes downregulation of Helicobacter pylori adhesin genes sabA and labA while dampening the production of proinflammatory cytokines.

Chronic inflammation induced by Helicobacter pylori is strongly associated with gastric cancer development, which is influenced by both bacterial virulence and host genetics. The sialic acid-binding adhesin SabA and the MUC5AC-binding adhesin LabA are important H. pylori virulence factors that facilitate adhesion of the bacterium, which is a crucial step in colonization.

Role of Sortase A in Kx110A1 Adhesion to Gastric Epithelial Cells and Competitive Exclusion of .

We have previously shown that Kx110A1, a human stomach isolate, can colonize mouse stomach and reduce the initial colonization of . Here, we investigated the role of sortase-dependent proteins (SDPs) involved in these functions by the construction of a mutant for , the gene encoding the housekeeping sortase that covalently anchors SDPs to the cell surface. The mutant showed a decrease in hydrophobicity and autoaggregation under acidic conditions, indicating the effect of SDPs on cell surface properties.

Suppresses the Production of Proinflammatory Cytokines in -Infected Macrophages by Inhibiting the Expression of ADAM17.

The ability of to evade the host immune system allows the bacterium to colonize the host for a lifetime. Long-term infection with causes chronic inflammation, which is the major risk factor for the development of gastric ulcers and gastric cancer. Lactobacilli are part of the human microbiota and have been studied as an adjunct treatment in eradication therapy.