Redox dynamics in seeds of spp: unraveling adaptation strategies of different seed categories.

Background: Seeds of woody plant species, such as those in the like Norway maple ( L.) and sycamore ( L.), exhibit unique physiological traits and responses to environmental stress.

High-throughput method for Oxygen Consumption Rate measurement (OCR) in plant mitochondria.

Background: Conventional methods to measure oxygen consumption, such as Clark-type electrodes, have limitations such as requiring a large amount of starting material. Moreover, commercially available kits for high-throughput methods are usually optimized for animal cells and mitochondria. Here, we present a novel method to measure the oxygen consumption rate using a high-throughput assay in isolated mitochondria of European beech seeds.

The chances in the redox priming of nondormant recalcitrant seeds by spermidine.

The problems posed by seed sensitivity to desiccation and aging have motivated the development of various techniques for mitigating their detrimental effects. The redox priming of seeds in antioxidant solution to improve their postharvest performance is one of the approaches. Spermidine (Spd) was tested as an invigorating solution on nondormant recalcitrant (desiccation-sensitive) seeds of the silver maple (Acer saccharinum L.

Assessment of ITS2 Region Relevance for Taxa Discrimination and Phylogenetic Inference among Pinaceae.

The internal transcribed spacer 2 (ITS2) is one of the best-known universal DNA barcode regions. This short nuclear region is commonly used not only to discriminate taxa, but also to reconstruct phylogenetic relationships. However, the efficiency of using ITS2 in these applications depends on many factors, including the family under study.

New Insight into Taxonomy of European Mountain Pines, Complex, Based on Complete Chloroplast Genomes Sequencing.

The complex is a large group of closely related mountain pines, which are an important component of the ecosystems of the most important mountain ranges, such as the Alps, Carpathians and Pyrenees. The phylogenetic relationships between taxa in this complex have been under discussion for many years. Despite the use of many different approaches, they still need to be clarified and supplemented with new data, especially those obtained with high-throughput methods.