Study of pesticide transformation processes in different wheat varieties and their effects on plant metabolism.

Background: This study aims to obtain systematic understanding of the way by which pesticides are metabolized in plants and the influence of this process on plants' metabolism as this process has a key impact on plant-based food safety and quality. The research was conducted under field conditions, which enabled to capture metabolic processes taking place in plants grown under multihectare cultivation conditions.

Results: Research was conducted on three wheat varieties cultivated under field conditions and treated by commercially available preparations (fungicides, herbicides, insecticides, and growth regulator).

Non-target metabolomics approach for the investigation of the hidden effects induced by atrazine and its degradation products on plant metabolism.

Japanese radish (Raphanus sativus var. longipinnatus) plants grown under laboratory conditions were individually exposed to the same doses of atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine, ATR) or its main degradation products: either 2-amino-4-chloro-6-isopropylamino-1,3,5-triazine (DEA) or 2-amino-4-chloro-6-ethylamino-1,3,5-triazine (DIA) or desethyl-desisopropyl-atrazine (DEDIA) or 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine (HA), respectively. One week after treatment in plants exposed to ATR, DIA, and DEA, their concentrations were 7.

Comprehensive multiresidue chromatographic methods for monitoring pesticides in agricultural areas and corresponding plant protection zones.

This article reports a comprehensive analytical method for the identification and quantification of a broad range of pesticides in green plant crops. The sample preparation method for pesticides involved an optimization of the QuEChERS-based extraction protocol, with sample mass, volume of added water, and the type of cleanup sorbent as variables. A sorbent combination based on ENVI-Carb and ChloroFiltr was examined.

Metabolic profiling to evaluate the impact of amantadine and rimantadine on the secondary metabolism of a model organism.

Metabolic profiling offers huge potential to highlight markers and mechanisms in support of toxicology and pathology investigations during drug development. The main objective was to modify therapy with adamantane derivatives: amantadine and rimantadine, to increase their bioavailability and evaluate the influence of such therapy on drug metabolism using Saccharomyces cerevisiae as the model organism. In this study, the profile of endogenous metabolites of a model organism was measured and interpreted to provide an opportunity to investigate changes induced by treatment with amantadine and rimantadine.

The metabolic processes of selected pesticides and their influence on plant metabolism. A case study of two field-cultivated wheat varieties.

Pesticides that are absorbed by plants undergo biotransformation and might affect plant metabolic processes. The metabolisms of two cultivated wheat varieties, Fidelius and Tobak, treated with commercially available fungicides (fluodioxonil, fluxapyroxad, and triticonazole) and herbicides (diflufenican, florasulam, and penoxsulam) were studied under field conditions. The results provide novel insights regarding the effects of these pesticides on plant metabolic processes.

Metabolic profiles and fingerprints for the investigation of the influence of nitisinone on the metabolism of the yeast Saccharomyces cerevisiae.

Nitisinone (2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione, NTBC) is considered a potentially effective drug for the treatment of various metabolic diseases associated with disorders of L-tyrosine metabolism however, side-effects impede its widespread use. This work aimed to broaden the knowledge of the influence of NTBC and its metabolites 2-amino-4-(trifluoromethyl)benzoic acid (ATFA), 2-nitro-4-(trifluoromethyl)benzoic acid (NTFA), and cyclohexane-1,3-dione (CHD) on the catabolism of L-tyrosine and other endogenous compounds in Saccharomyces cerevisiae. Based on a targeted analysis performed by LC-ESI-MS/MS, based on multiple reaction monitoring, it was found that the dissipation kinetics of the parent compound and its metabolites are compatible with a first-order reaction mechanism.

Targeted and non-targeted analysis for the investigation of pesticides influence on wheat cultivated under field conditions.

A comprehensive approach was applied to evaluate the effects of pesticides on the metabolism of wheat (Triticum aestivum L). The application of commercially available pesticide formulations under field cultivation conditions provided a source of metabolic data unlimited by model conditions, representing a novel approach to study the effects of pesticides on edible plants. Gas and liquid chromatography coupled to tandem mass spectrometry were employed for targeted and non-targeted analysis of wheat roots and shoots sampled six times during the six-week experiment.

β-Cyclodextrin Derivative Grafted on Silica Gel Represents a New Polymeric Sorbent for Extracting Nitisinone from Model Physiological Fluids.

Nitisinone (NTBC) is used in the treatment of disorders affecting the tyrosine pathway, including hereditary tyrosinemia type I, alkaptonuria, and neuroblastoma. An inappropriate dosage of this therapeutic drug causes side effects; therefore, it is necessary to develop a rapid and sensitive method to monitor the content of NTBC in patients' blood. This study aimed to develop anew polymeric sorbent containing β-cyclodextrin (β-CD) derivatives grafted on silica gel to effectively extract NTBC from model physiological fluids.

Influence of nitisinone and its metabolites on l-tyrosine metabolism in a model system.

Nitisinone (NTBC) is currently used for the treatment of tyrosinemia type 1, a rare disease. It also exhibits potential in the treatment of other orphan diseases as well as nervous system disorders - this is however limited by its side effects. In all living organisms, NTBC inhibits 4-hydroxyphenylpyruvate dioxygenase activity, thereby affecting l-tyrosine (L-TYR) catabolism, which results in the therapeutic effect.

Metabolic profiles and non-targeted LC-MS/MS approach as a complementary tool to targeted analysis in assessment of plant exposure to pesticides.

Liquid chromatography coupled with tandem mass spectrometry was employed for the detection of pesticides (thiamethoxam, lambda-cyhalothrin, deltamethrin, and metalaxyl) and their metabolites in Raphanus sativus var. longipinnatus exposed to these compounds under experimental conditions. Metalaxyl (0.

Rapid MSPD-LC-MS/MS Procedure for Determination of Pesticides in Potato Tubers.

The program of potato protection recommended by the producers of agrochemicals requires application: thiamethoxam, lambda-cyhalothrin, deltamethrin, rimsulfuron and metalaxyl. Therefore, there is a risk that these pesticides are present in tubers, thus posing a toxicological risk to the consumer. In this respect, it is necessary to monitor the presence of these compounds in edible plants.

Profiling and fingerprinting strategies to assess exposure of edible plants to herbicides.

Raphanus sativus var. longipinnatus, was exposed under experimental conditions to herbicides: rimsulfuron (RIM), administrated as (1) pure substance, (2) in commercially available formulation (RIMEL), (3) its degradation product: 4,6-dimethoxypyrimidin-2-amine (2ADP), (4) mesotrione (MES), (5) sulcotrione (SUL). Profiling and fingerprinting strategies, conducted by LC-MS/MS-FL, were employed to find markers of plant exposure to herbicide stress.

Metabolic profiles in the course of the shikimic acid pathway of Raphanus sativus var. longipinnatus exposed to mesotrione and its degradation products.

The influence of pesticides on the metabolism of edible plants has not been fully investigated. Moreover, once introduced into the environment, pesticides are degraded to many compounds with undefined bioactivity. In presented work, under experimental conditions, model edible plant (Raphanus sativus var.

Potential application of for the phytoremediation of mesotrione and its degradation products from water.

The aim of the present work is to estimate remediation potential of , its ability to uptake mesotrione (MES) - one of the most frequently used herbicides, and its main degradation products: 2-amino-4-methylsulfonyl benzoic acid (AMBA) and 4-methylsulfonyl-2-nitrobenzoic acid (MNBA). This research focuses on model experiments performed under laboratory conditions. The results show that can uptake up to 75% of degradation products from 1 L of surface water samples polluted with 0.

LC-MS/MS study of the degradation processes of nitisinone and its by-products.

Nitisinone (2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione, NTBC) was the first synthetically produced triketone herbicide. However, its unsatisfactory herbicidal properties, negative impact on the natural environment and the high cost of synthesis have hindered its commercialization as a plant protection agent. Nevertheless, NTBC has become the medical treatment of choice for a rare hereditary metabolic disease -hepatorenal tyrosinemia.

Review of Sample Preparation Techniques for the Analysis of Selected Classes of Pesticides in Plant Matrices.

The aim of this article is to present the trends in extraction techniques applied for the isolation of pesticides from plant matrix. To fully compare the effectiveness of different extraction techniques, it was required to analyze compounds with possibly wide spectrum of physicochemical properties. Hence, compounds representing neonicotinoids, pyrethroids, sulfonylureas and phenylamides were selected.

Atrazine, triketone herbicides, and their degradation products in sediment, soil and surface water samples in Poland.

The aim of this study was to monitor the sediment, soil and surface water contamination with selected popular triketone herbicides (mesotrione (MES) and sulcotrione(SUL)), atrazine (ATR) classified as a possible carcinogen and endocrine disrupting chemical, as well as their degradation products, in Silesia (Poland). Seventeen sediment samples, 24 soil samples, and 64 surface water samples collected in 2014 were studied. After solid-liquid extraction (SLE) and solid phase extraction (SPE), analytes were determined by high-performance liquid chromatography (HPLC) with diode array detection (DAD).

Extraction procedures for the study of phytotoxicity and degradation processes of selected triketones in a water ecosystem.

Simple and effective extraction methods based on matrix solid-phase dispersion (MSPD), dispersive liquid-liquid microextraction (DLLME), and solid-phase extraction (SPE) coupled with high-performance liquid chromatography with diode array detector (HPLC-DAD) were developed to determine triketone herbicides-sulcotrione (SUL), mesotrione (MES), tembotrione (TEMB), and their degradation products-in plant tissues and water samples. The extraction procedures were employed to enable quantification of the accumulation of selected triketone herbicides and their degradation products in a model aquatic plant, Egeria densa. To obtain comprehensive information about the triketones' influence on an aquatic plant, changes in chlorophyll concentration in plants exposed to these triketones were monitored.

Monitoring of atrazine in milk using a rapid tube-based ELISA and validation with HPLC.

Although atrazine has been banned in the European Union since 2007 it still persists in soil from where it can enter the food chain. Milk-producing animals accumulate atrazine from contaminated feed and water and since large quantities of milk and milk products are consumed its quality should be constantly monitored. The objective of this investigation was to develop a simple tube ELISA procedure suitable for use in non-specialised laboratories and in the field.

New procedures for simultaneous determination of mesotrione and atrazine in water and soil. Comparison of the degradation processes of mesotrione and atrazine.

A method for the determination of residues of mesotrione, atrazine and its degradation products: deethylatrazine, hydroxyatrazine, deisopropylatrazine, desethyldesisopropylatrazine in a variety of water and soil matrices has been developed. Mesotrione is a new selective herbicide for use in corn, which has been substituted for atrazine, which has been banned in European Union countries since 2007. Although atrazine has not been used for three vegetative periods, it is still detected in the environment.

Procedures for analysis of atrazine and simazine in environmental matrices.

There is an ongoing need to monitor soil and trophic chain samples for residues of triazine herbicides, particularly atrazine and simazine, because these herbicides are among the most used members of their class, are toxic, can be persistent, and are widely distributed in the environment. The main purpose of this review is to provide an overview of principle techniques and approaches used in analyzing atrazine, simazine, and other triazine herbicide residues in environmental matrices. The methods covered generally provide low detection limits, acceptable levels of matrix interferences, and are relatively fast and inexpensive.

ELISA and HPLC methods for atrazine and simazine determination in trophic chains samples.

The aim of the research was to determine optimal conditions for atrazine determination in trophic chain samples by means of an antigen-coated tube enzyme-linked immunosorbent assay (ELISA). The ELISA method was used for analysis of a selection of samples and the results and method requirement compared with HPLC. The 2 h competitive ELISA showed a minimum detection limit of 0.

Procedures of trophic chain samples preparation for determination of triazines by HPLC and metals by ICP-AES methods.

The aim of this research was monitoring the distribution of atrazine and simazine as well as metals Pb, Cd, Zn, Al, Co, Ni, and V along with trophic chains: soil-vegetables and soil, carrot or grass and meat. Different techniques of herbicides extraction by means of many solvents were examined. Triazines were analysed by means of HPLC, metals by means of ICP-AES.

Distribution of pesticides and heavy metals in trophic chain.

Determination of triazines herbicides (atrazine and simazine) by high performance liquid chromatography (HPLC) in samples of trophic chain were worked out. Determination limits of 0.5 microg g(-1) for atrazine, 0.