Two new flavonol glycosides from the leaves of Cleome chelidonii L.f.

From the leaves of Cleome chelidonii L.f., two new flavonol glycosides, named cleomesides A (1) and B (2), and four known compounds, quercetin 3-O-β-glucopyranosyl(1 → 2)-α-rhamnoside-7-O-α-rhamnoside (3), ethyl α-galactopyranoside (4), adenine (5) and glycerol monostearate (6), were isolated.

Chemical constituents from the leaves of Manglietia phuthoensis and their effects on osteoblastic MC3T3-E1 cells.

Two new phenyl glycosides, mangliesides A and B (1, 2), a new ionol glycoside, manglieside C (3), two new lignan glycosides, mangliesides D and E (4, 5), were isolated from the leaves of Manglietia phuthoensis, along with two known lignans, 3-methoxymagnolol (6) and obovatol (7). Their structures were established by means of 1D and 2D NMR, electrospray ionization (ESI)-MS and HR-ESI-MS experiments. Among them, compounds 2 and 5 significantly (p<0.

Synchronous-scan fluorescence of algal cells for toxicity assessment of heavy metals and herbicides.

Synchronous-scan spectrofluorometry was applied to Chlorella vulgaris cells to assess the toxicity of heavy metals and herbicides in water. Simultaneous scan of both the excitation and emission spectra was done at a constant wavelength difference Deltalambda (20-140 nm) between the emission and excitation wavelengths in the range of 420-700 nm emission, where a peak of fluorescence was observed. Its position depends on Deltalambda.

Fluorescent biosensor using whole cells in an inorganic translucent matrix.

An optical biosensor based on vegetal cells entrapped in an inorganic translucent matrix and fluorescence detection has been developed. The biosensor uses Chlorella vulgaris immobilized in a translucent support produced from sol-gel technology. The translucence of the structure enables the algal active layer to be placed directly in contact with the optical fibers for fluorescence detection.