High-throughput MALDI-MSI metabolite analysis of plant tissue microarrays.

A novel metabolomics analysis technique, termed matrix-assisted laser desorption/ionization mass spectrometry imaging-based plant tissue microarray (MALDI-MSI-PTMA), was successfully developed for high-throughput metabolite detection and imaging from plant tissues. This technique completely overcomes the disadvantage that metabolites cannot be accessible on an intact plant tissue due to the limitations of the special structures of plant cells (e.g.

Effect of different plant growth regulators on callus and adventitious shoots induction, polysaccharides accumulation and antioxidant activity of .

Objective: As a medicinal plant, the resource of is deficient along with the large collection. For the protection and utilization of , the influence of plant growth regulators (PGRs) on callus induction and adventitious shoots differentiation, polysaccharide production and the antioxidant activity were tested.

Methods: Internodes of were used as explants and cultured on MS medium plus different plant growth regulators (PGRs).

UPLC-MS-Based Non-targeted Analysis of Endogenous Metabolite Changes in the Leaves of Grüning Induced by 6-Benzylaminopurine and Kinetin.

propagation technology with plant growth regulators (PGRs) is generally applied in the cultivation of , which can solve collection difficulties and limited resources of . Nevertheless, comprehensive metabolomic evaluation on with PGR effects is still lacking. In this work, a non-targeted metabolomics approach, coupled with statistical and pathway enrichment analysis, was used to assess the regulatory influences of 6-benzylaminopurine (6-BA) and kinetin (KT) applied in .