Publications by authors named "Hang-jun Lv"

Unlabelled: , which causes toxoplasmosis, is prevalent in warm-blooded animals, such as cats, dogs, and humans. causes economic losses to livestock production and represents a potential risk to public health. Dogs and cats are common hosts in the epidemiology of toxoplasmosis.

View Article and Find Full Text PDF

Objective: To explore the molecular basis of an individual featuring an ABx variant of ABO blood group system.

Methods: Serological assays were used to characterize the erythrocyte phenotypes and salivary ABH secretors. All of the seven exons and flanking introns of ABO glycosyltransferase gene were amplified with polymerase chain reaction (PCR).

View Article and Find Full Text PDF

Objective: To discriminate and analyze the relative frequencies of alleles in HLA-DRB1*12:01:01G(HLA-DRB1*12:01:01/12:06/12:10/12:17) and HLA-DRB1*14:01:01G (DRB1*14:01:01/14:54) groups and assess their associations with HLA-DRB3 and HLA-DQB1 loci.

Methods: A total of 115 DNA samples previously typed as HLA-DRB1*12:01:01G and 108 samples from HLA-DRB1*14:01:01G were selected. DNA sequences for exons 1 to 3 of the HLA-DRB1 locus were analyzed for HLA-DRB1*12:01:01G, and exons 2 to 3 were analyzed for HLA-DRB1*14:01:01G by polymerase chain reaction sequence-based typing (PCR-SBT).

View Article and Find Full Text PDF

Objective: To analyze the molecular basis for an individual with ABx09 phenotype of ABO subtype.

Methods: The ABO group antigens on red blood cells of the proband were identified by monoclonal antibodies, and the ABO antibody in serum was detected by standard A, B, O cells. The exons 1 to 7 of ABO gene were amplified by polymerase chain reaction (PCR) respectively and the PCR products were sequenced directly.

View Article and Find Full Text PDF
Article Synopsis
  • - The study aimed to develop an eukaryotic cell expression system for the FUT1 gene and investigate how mutations (293C to T and 658C to T) affect H antigen expression.
  • - Genomic DNA from individuals with the para-Bombay phenotype was used to amplify the FUT1 gene, which was then ligated into a plasmid and transfected into COS-7 cells for analysis.
  • - Results showed that while RNA and protein levels of the FUT1 variants were comparable to wildtype, the enzyme activity was significantly reduced in mutant cells, leading to decreased H antigen expression.
View Article and Find Full Text PDF

Objective: To investigate the recombination events between human leukocyte antigen (HLA) loci within two families.

Methods: Identification of HLA-A, -C, -B, -DRB1 and -DQB1 loci was firstly carried out using polymerase chain reaction-sequence specific oligonucleotide. Then HLA high resolution typing was performed using polymerase chain reaction sequencing-based typing.

View Article and Find Full Text PDF

Objective: To elucidate the serological characteristics and molecular mechanism of a blood donor with weaken B antigen.

Methods: The ABO blood group antigens on red blood cells were identified by monoclonal antibodies, the ABO antibodies in serum were detected by standard A, B, O cells and the activity of the B glycosyltransferase was analyzed. The full-length sequence and 5'-untranslated region (5'-UTR) sequence of ABO gene were amplified by polymerase chain reaction (PCR) respectively and direct sequencing.

View Article and Find Full Text PDF
Article Synopsis
  • - The study aimed to analyze the exons 2-4 of a new human leukocyte antigen (HLA) allele, specifically HLA-B*15:129.
  • - DNA was extracted from a blood sample and amplified using PCR, followed by sequencing, which revealed the presence of both a known allele (B*07:02) and the novel allele.
  • - The novel allele, B*15:129, was found to have three nucleotide differences compared to a closely related allele, leading to a specific amino acid change, and has been documented in GenBank.
View Article and Find Full Text PDF

Objective: To clone and investigate the polymorphism of the 5'-untranslated region (5'-UTR) of the human ABO gene, in order to provide the basis for exploring the transcriptional regulation of the human ABO histo-blood group genes.

Methods: ABO phenotypes of 30 unrelated healthy blood donors were determined by serological technique, their genotypes were analyzed by sequencing the exons 6 and 7 of the ABO gene. Nearly 5 kb of the 5'-UTR of ABO gene was obtained by PCR amplification and sequencing was performed bidirectionally.

View Article and Find Full Text PDF

Objective: To investigate the polymorphisms of platelet membrane glycoprotein genes related to human platelet alloantigen (HPA)-1 to 17w.

Methods: The DNA segments of platelet membrane glycoprotein genes related to HPA-1 to 17w were amplified using author's designed primers. The amplification products were purified and directly sequenced to identify the HPA genotype and glycoprotein gene polymorphisms.

View Article and Find Full Text PDF

Objective: To establish the allele specific primer polymerase chain reaction sequence-based typing (PCR-SBT) and investigate the polymorphism of exon 3 of human leukocyte antigen( HLA)-DRB1.

Methods: The fragment containing exons 2 and 3 of HLA-DRB1 gene was amplified by group specific primers. The amplified products were digested by restriction enzymes and directly sequenced in both directions.

View Article and Find Full Text PDF

Objective: To investigate the molecular genetic basis of para-Bombay phenotype in a Chinese family.

Methods: ABO and H phenotypes of the proband and his pedigree were characterized by serological techniques. The exons 6 and 7 of the ABO gene and full coding region of alpha-1,2-fucosyltransferase (FUT1) gene of the pedigree were analyzed by polymerase chain reaction and direct sequencing of the amplified fragments.

View Article and Find Full Text PDF

Objective: To investigate the molecular genetic basis of the B3 variant of ABO blood group system with mixed-field hemagglutination in Chinese.

Methods: Serological techniques were performed to characterize the erythrocyte phenotype of two discrepant samples. A sequential agglutination method and 13 short tandem repeat (STR) loci were tested to exclude the possibility of exogenous or endogenous DNA chimera.

View Article and Find Full Text PDF

We investigated the molecular genetic basis of rare cisAB variants at the ABO locus in Chinese population. Serological techniques were performed to characterize erythrocyte phenotype of 12 discrepant samples and 116 randomly selected samples. Mutations of complete exon 6 and 7 including flanking intron in the ABO genes were screened by PCR and directly sequencing.

View Article and Find Full Text PDF

A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_sessionl44ce9isa0heqglmm6f45puhu0kv877t): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once