Zhonghua Shao Shang Za Zhi
February 2007
Objective: To investigate the possibility of differentiation of human mesenchymal stem cells (hMSC) into epidemic cells in vitro.
Methods: hMSCs were segregated from normal adult human bone marrow by Percoll solution (1.073 g/ml) , and were cultured, purified, and amplified to 3th passage in vitro.
Zhejiang Da Xue Xue Bao Yi Xue Ban
April 2003
Objective: To isolate MSCs from adult human bone marrow cells and to induce them into adipocytes.
Methods: MSCs were isolated from adult human bone marrow aspirated by Percoll and expanded in L-DMEM. The surface antigen of MSCs, CD14, CD34, CD45, CD44, VLA-1, HLA-DR and cell cycle were analysed on a FACScan flow cytometer.