An aggregation-induced emission-active lysosome hijacker: Sabotaging lysosomes to boost photodynamic therapy efficacy and conquer tumor therapeutic resistance.

Therapeutic resistance is a major challenge in clinical cancer theranostics, often leading to treatment failure and increased patient mortality. Breaking this therapeutic deadlock, enhancing the efficacy of clinical treatments, and ultimately improving patient survival rates are both highly desirable and significantly challenging goals. Herein, we have developed a new fluorescent luminogen, QM-DMAC, which features aggregation-induced emission (AIE), and exceptional viscosity-responsive properties.

Regulation of chitin synthesis by the juvenile hormone analogue fenoxycarb in Hyphantria cunea.

Fenoxycarb, a non-terpenoid carbamate with stomach and contact toxicity, is a promising insecticide affecting insect growth and development. However, fenoxycarb how to regulate insect digestion and absorption and chitin synthesis remains largely unclear. Here, we investigated the effects of fenoxycarb on growth, chitin synthesis, carbohydrate homeostasis, and digestive capabilities in Hyphantria cunea, a widespread agricultural and forestry pest, to clarify the action mechanism of fenoxycarb from the perspective of digestive function and carbohydrate metabolism, and confirmed that fenoxycarb significantly decreased chitin content, increased chitinase activity, and regulated the expression of genes related to chitin synthesis and degradation.

mRNA-LNP vaccines combined with tPA signal sequence elicit strong protective immunity against .

is a prominent Gram-negative and encapsulated opportunistic pathogen that causes a multitude of infections such as severe respiratory and healthcare-associated infections. Despite the widespread anti-microbial resistance and the high mortality rate, currently, no clinically vaccine is approved for battling . To date, messenger RNA (mRNA) vaccine is one of the most advancing technologies and are extensively investigated for viral infection, while infrequently applied for prevention of bacterial infections.

Anti-hepatoma effect of homologous delivery of doxorubicin by HepG2 cells.

Compared to conventional polymer-based and biomaterial carriers, cells as vehicles for delivering bioactive molecules in the treatment of tumor diseases offer characteristics such as non-toxicity, biocompatibility, low immunogenicity, and prolonged in vivo circulation. However, the focus of current cell drug delivery systems predominantly lies on live cells, such as red blood cells, white blood cells and others. Here, a drug delivery strategy targeting liver cancer utilizing cryo-shocked liver cancer cells (HepG2) as carriers was presented, and non-proliferative HepG2 cells particles loaded with DOX (HepG2-DOX) was effectively prepared, which has good homologous targeting.

Phosphine and phosphine plus ethyl formate for controlling papaya mealybug (Hemiptera: Pseudococcidae) on succulents.

The papaya mealybug, Paracoccus marginatus (Hemiptera: Pseudococcidae), poses a serious threat to global trade and is classified as a quarantine pest in some countries. Phosphine (PH3) is considered an alternative to methyl bromide for postharvest control of P. marginatus.

Ultrafast and Highly Efficient Laser Extraction of Matrine and Oxymatrine from for the Anticancer Activity.

Matrine and oxymatrine are mainly obtained from using the high-temperature and prolonged solvent extraction methods currently employed in industries. In this study, an ultrafast and highly efficient method for extracting matrine and oxymatrine from at room temperature using laser technology, specifically, laser extraction, was demonstrated. The laser extraction rates for matrine and oxymatrine from at room temperature for 1 min were 266.

Establishment of acquired tracheoesophageal fistula using a modified magnetic compression technique in rabbits and its postmodeling evaluation.

Background: Previous studies have validated the efficacy of both magnetic compression and surgical techniques in creating rabbit tracheoesophageal fistula (TEF) models. Magnetic compression achieves a 100% success rate but requires more time, while surgery, though less frequently successful, offers rapid model establishment and technical maturity in larger animal models.

Aim: To determine the optimal approach for rabbit disease modeling and refine the process.

Model Selection for Exponential Power Mixture Regression Models.

Finite mixture of linear regression (FMLR) models are among the most exemplary statistical tools to deal with various heterogeneous data. In this paper, we introduce a new procedure to simultaneously determine the number of components and perform variable selection for the different regressions for FMLR models via an exponential power error distribution, which includes normal distributions and Laplace distributions as special cases. Under some regularity conditions, the consistency of order selection and the consistency of variable selection are established, and the asymptotic normality for the estimators of non-zero parameters is investigated.

Soil Nitrogen and Flooding Intensity Determine the Trade-Off between Leaf and Root Traits of Riparian Plant Species.

The investigation into trade-offs among plant functional traits sheds light on how plants strategically balance growth and survival when facing environmental stress. This study sought to evaluate whether trade-offs observed at both community and individual species levels could indicate adaptive fitness across an intensity of flooding intensity. The study was conducted at 25 sampling sites spanning approximately 600 km along the riparian zone in the Three Gorges Reservoir area, China.

[Retrospective Study of Bronchoscopic Intervention Therapy for Bronchopleural Fistula Induced by Pulmonary Surgery].

Background: As a new technique developed in recent years, bronchoscopic intervention therapy has the advantages of minimal invasion, high safety and repeatability. The aim of this study is to investigate the clinical characteristics of bronchopleural fistula (BPF) induced by surgeries for lung malignancies or benign diseases and the effect of bronchoscopic intervention therapy for BPF, so as to provide support for prevention and treatment of BPF.

Methods: Data 64 patients with BPF who were treated by bronchoscopic intervention in Respiratory Disease Center of Dongzhimen Hospital, Beijing University of Chinese Medicine from June 2020 to September 2023 were collected.

Development and Metabolomic Profiles of (Diptera: Tephritidae) Larvae Exposed to Phytosanitary Irradiation Dose in Hypoxic Environment Using DI-SPME-GC/MS.

X-ray irradiation and modified atmospheres (MAs) provide eco-friendly, chemical-free methods for pest management. Although a low-oxygen atmospheric treatment improves the performance of some irradiated insects, its influence on the irradiation of quarantine insects and its impacts on pest control efficacy have yet to be investigated. Based on bioassay results, this study employed direct immersion solid-phase microextraction (DI-SPME) combined with gas chromatography-mass spectrometry (GC-MS) to determine metabolic profiles of late third-instar larvae under normoxia (CON, Air), hypoxia (95% N + 5% O, HY), super-hypoxia (99.

[A Case of Multidomain Integrated Treatment Strategy for Complex Primary Pulmonary Sarcomatoid Carcinoma].

Pulmonary sarcomatoid carcinoma (PSC) is a rare and highly malignant tumor, which includes the following five pathologic types: pleomorphic carcinoma, spindle cell carcinoma, giant cell carcinoma, carcinosarcoma and pulmonary blastoma. The onset of PSC is occult with non-specific clinical symptoms and signs. The clinical manifestations include irritating cough, bloody sputum, dyspnea, chest pain and so on, which are closely related to the growth and invasion site of the tumor.

Phenotypic heterogeneity unveils a negative correlation between antibiotic resistance and quorum sensing in clinical isolates.

Colonization of in the lung environments frequently leads to the enrichment of strains displaying enhanced antibiotic resistance and reduced production of quorum-sensing (QS) controlled products. However, the relationship between the emergence of QS deficient variants and antibiotic resistance remains less understood. In this study, 67 strains were isolated from the lungs of 14 patients with chronic obstructive pulmonary disease, followed by determining their genetic relationship, QS-related phenotypes and resistance to commonly used antibiotics.

On-chip hybrid integration of swept frequency distributed-feedback laser with silicon photonic circuits using photonic wire bonding.

This paper presents a novel co-packaging approach through on-chip hybrid laser integration with photonic circuits using photonic wire bonding. The process involves die-bonding a low-cost semiconductor distributed-feedback (DFB) laser into a deep trench on a silicon-on-insulator (SOI) chip and coupling it to the silicon circuitry through photonic wire bonding (PWB). After characterizing the power-current-voltage (LIV) and optical spectrum of the laser, a wavelength-current relationship utilizing its tunability through self-heating a swept-frequency laser (SFL) is developed.

Influence of forest vegetation restoration on carbon increment after mining.

We have clarified the study area has a history of 65 years and has been restored for 6 years. This study investigated the carbon storage characteristics of undisturbed natural forests and restored mining vegetation in Yunnan Province, China. The goal was to quantify carbon reserves and increments to inform ecological restoration strategies.

FDP-Na-induced enhancement of glycolysis impacts larval growth and development and chitin biosynthesis in fall webworm, Hyphantria cunea (Lepidoptera: Arctiidae).

Fructose 1, 6-diphosphate (FDP) is an endogenous intermediate in the glycolytic pathway, as well as an allosteric activator of phosphofructokinase (PFK). Based on the role in promoting glycolysis, FDP has been widely used as a therapeutic agent for mitigating the damage of endotoxemia and ischemia/reperfusion in clinical practice. However, the effect of exogenous FDP-induced glycolysis activation on insect carbohydrate metabolism and chitin synthesis remains largely unclear.

Advances in Polysaccharide Production Based on the Co-Culture of Microbes.

Microbial polysaccharides are natural carbohydrates that can confer adhesion capacity to cells and protect them from harsh environments. Due to their various physiological activities, these macromolecules are widely used in food, medicine, environmental, cosmetic, and textile applications. Microbial co-culture is an important strategy that is used to increase the production of microbial polysaccharides or produce new polysaccharides (structural alterations).

Recent Experimental Advances in Characterizing the Self-Assembly and Phase Behavior of Polypeptoids.

Polypeptoids are a family of synthetic peptidomimetic polymers featuring N-substituted polyglycine backbones with large chemical and structural diversity. Their synthetic accessibility, tunable property/functionality, and biological relevance make polypeptoids a promising platform for molecular biomimicry and various biotechnological applications. To gain insight into the relationship between the chemical structure, self-assembly behavior, and physicochemical properties of polypeptoids, many efforts have been made using thermal analysis, microscopy, scattering, and spectroscopic techniques.

S1PR1 attenuates pulmonary fibrosis by inhibiting EndMT and improving endothelial barrier function.

Background: Idiopathic pulmonary fibrosis (IPF) is a chronic fatal disease of unknown etiology. Its pathological manifestations include excessive proliferation and activation of fibroblasts and deposition of extracellular matrix. Endothelial cell-mesenchymal transformation (EndMT), a novel mechanism that generates fibroblast during IPF, is responsible for fibroblast-like phenotypic changes and activation of fibroblasts into hypersecretory cells.

Flowthrough Capture of Microplastics through Polyphenol-Mediated Interfacial Interactions on Wood Sawdust.

Nano-/microplastics accumulate in aquatic bodies and raise increasing threats to ecosystems and human health. The limitation of existing water cleanup strategies, especially in the context of nano-/microplastics, primarily arises from their complexity (morphological, compositional, and dimensional). Here, highly efficient and bio-based flowthrough capturing materials (bioCap) are reported to remove a broad spectrum of nano-/microplastics from water: polyethylene terephthalate (anionic, irregular shape), polyethylene (net neutral, irregular shape), polystyrene (anionic and cationic, spherical shape), and other anionic and spherical shaped particles (polymethyl methacrylate, polypropylene, and polyvinyl chloride).

[Effect of polymer nanoparticles on atherosclerotic lesions and the associated mechanisms: a review].

Polymer nanoparticles generally refer to hydrophobic polymers-based nanoparticles, which have been extensively studied in the nanomedicine field due to their good biocompatibility, efficient long-circulation characteristics, and superior metabolic discharge patterns over other nanoparticles. Existing studies have proved that polymer nanoparticles possess unique advantages in the diagnosis and treatment of cardiovascular diseases, and have been transformed from basic researches to clinical applications, especially in the diagnosis and treatment of atherosclerosis (AS). However, the inflammatory reaction induced by polymer nanoparticles would induce the formation of foam cells and autophagy of macrophages.

Gingipains are the important virulence factors of Porphyromonas gingivalis downregulating B10 cells.

Porphyromonas gingivalis is a keystone pathogen in periodontitis. Our previous study indicated that periodontitis induced by P. gingivalis increased the percentage of CD19 B cells but decreased the ratio of IL-10-producing regulatory B cells (B10) in collagen-induced arthritis (CIA) mice.

A molecular dynamics and quantum mechanical investigation of intermolecular interaction and electron-transfer mechanism between copper-containing nitrite reductase and redox partner pseudoazurin.

Much of biological electron transfer occurs between proteins. These molecular processes usually involve molecular recognition and intermolecular electron transfer (inter-ET). The inter-ET reaction between (CuNiR) and partner protein (PAz) is the first step in denitrification, which is affected by intermolecular association.