SMARCA4 Inhibits Breast Cancer Progression and Metastasis through RHOA Suppression.

Triple-negative breast cancer (TNBC) presents the most challenging subtype of the disease due to its aggressive nature and lack of targeted therapy options. To identify regulators of TNBC, we conducted a genome-wide CRISPR-knockout screen in both 3D tumor spheroid and 2D cell culture models. The 3D spheroid model displayed unique potential in identifying putative tumor suppressors due to its closer mimicry of in vivo tumor growth conditions.

Knowledge, attitude and practice towards multiple myeloma among medical staff in Enshi Region.

A study in the Enshi Region between Sept-Nov 2023 assessed medical staff's knowledge, attitude, and practice regarding multiple myeloma. The disease significantly impacts physical health, quality of life, and mental well-being. Medical professionals play crucial roles in its prevention and treatment.

Integrated proteogenomic characterization of ampullary adenocarcinoma.

Ampullary adenocarcinoma (AMPAC) is a rare and heterogeneous malignancy. Here we performed a comprehensive proteogenomic analysis of 198 samples from Chinese AMPAC patients and duodenum patients. Genomic data illustrate that 4q loss causes fatty acid accumulation and cell proliferation.

Serum proteome reveals distinctive molecular features of H7N9- and SARS-CoV-2-infected patients.

The coronavirus disease 2019 (COVID-19) pandemic has reminded us of human infections with the H7N9 virus and has raised questions related to the clinical and molecular pathophysiological diversity between the two diseases. Here, we performed a proteomic approach on sera samples from patients with H7N9-virus or SARS-CoV-2-virus infection and healthy controls. Compared to SARS-CoV-2, H7N9-virus infection caused elevated neutrophil concentrations, T cell exhaustion, and increased cytokine/interleukin secretion.

Proteogenomic insights into the biology and treatment of pan-melanoma.

Melanoma is one of the most prevalent skin cancers, with high metastatic rates and poor prognosis. Understanding its molecular pathogenesis is crucial for improving its diagnosis and treatment. Integrated analysis of multi-omics data from 207 treatment-naïve melanomas (primary-cutaneous-melanomas (CM, n = 28), primary-acral-melanomas (AM, n = 81), primary-mucosal-melanomas (MM, n = 28), metastatic-melanomas (n = 27), and nevi (n = 43)) provides insights into melanoma biology.

Proteomic characterization identifies clinically relevant subgroups of soft tissue sarcoma.

Soft tissue sarcoma is a broad family of mesenchymal malignancies exhibiting remarkable histological diversity. We portray the proteomic landscape of 272 soft tissue sarcomas representing 12 major subtypes. Hierarchical classification finds the similarity of proteomic features between angiosarcoma and epithelial sarcoma, and elevated expression of SHC1 in AS and ES is correlated with poor prognosis.

An Investigation of the Energy Harvesting Capabilities of a Novel Three-Dimensional Super-Cell Phononic Crystal with a Local Resonance Structure.

Using the piezoelectric (PZT) effect, energy-harvesting has become possible for phononic crystal (PnC). Low-frequency vibration energy harvesting is more of a challenge, which can be solved by local resonance phononic crystals (LRPnCs). A novel three-dimensional (3D) energy harvesting LRPnC is proposed and further analyzed using the finite element method (FEM) software COMSOL.

Metal oxide nanoparticles embedded in porous carbon for sulfur absorption under hydrothermal conditions.

MO (M = Zn, Cu, Mn, Fe, Ce) nanoparticles (NPs) embedded in porous C with uniform diameter and dispersion were synthesized, with potential application as S-absorbents to protect catalysts from S-poisoning in catalytic hydrothermal gasification (cHTG) of biomass. S-absorption performance of MO/C was evaluated by reacting the materials with diethyl disulfide at HTG conditions (450 °C, 30 MPa, 15 min). Their S-absorption capacity followed the order CuO/C > CeO/C ≈ ZnO/C > MnO/C > FeO/C.

SARS-CoV-2 Omicron BA.5.2-infection and COVID-19 in persons with chronic myeloid leukaemia.

Objective: A SARS-CoV-2 Omicron (BA.5.2) epidemic began in China in December, 2022 following stopping the zero COVID policy.

Refining the time-frequency characteristic of non-stationary signal for improving time-frequency representation under variable speeds.

Time-frequency ridge not only exhibits the variable process of non-stationary signal with time changing but also provides the information of signal synchronous or non-synchronous components for subsequent detection research. Consequently, the key is to decrease the error between real and estimated ridge in the time-frequency domain for accurate detection. In this article, an adaptive weighted smooth model is presented as a post-processing tool to refine the time-frequency ridge which is based on the coarse estimated time-frequency ridge using newly emerging time-frequency methods.

A Domain Adaption ResNet Model to Detect Faults in Roller Bearings Using Vibro-Acoustic Data.

Intelligent fault diagnosis of roller bearings is facing two important problems, one is that train and test datasets have the same distribution, and the other is the installation positions of accelerometer sensors are limited in industrial environments, and the collected signals are often polluted by background noise. In the recent years, the discrepancy between train and test datasets is decreased by introducing the idea of transfer learning to solve the first issue. In addition, the non-contact sensors will replace the contact sensors.

Region-resolved multi-omics of the mouse eye.

The eye is a complex organ consisting of multiple compartments with unique and specialized properties, and small disturbances in one eye region can result in impaired vision and blindness. Although there have been advancements in ocular research, the hierarchical molecular network in region-wide resolution, indicating the division of labor and crosstalk among different eye regions, is not yet comprehensively illuminated. Here, we present an atlas of region-resolved proteome and lipidome of mouse eye.

Proteogenomics of diffuse gliomas reveal molecular subtypes associated with specific therapeutic targets and immune-evasion mechanisms.

Diffuse gliomas are devastating brain tumors. Here, we perform a proteogenomic profiling of 213 retrospectively collected glioma tumors. Proteogenomic analysis reveals the downstream biological events leading by EGFR-, IDH1-, TP53-mutations.

A proteogenomic analysis of clear cell renal cell carcinoma in a Chinese population.

Clear cell renal cell carcinoma (ccRCC) is a common and aggressive subtype of renal cancer. Here we conduct a comprehensive proteogenomic analysis of 232 tumor and adjacent non-tumor tissue pairs from Chinese ccRCC patients. By comparing with tumor adjacent tissues, we find that ccRCC shows extensive metabolic dysregulation and an enhanced immune response.

Signatures of TOP1 transcription-associated mutagenesis in cancer and germline.

The mutational landscape is shaped by many processes. Genic regions are vulnerable to mutation but are preferentially protected by transcription-coupled repair. In microorganisms, transcription has been demonstrated to be mutagenic; however, the impact of transcription-associated mutagenesis remains to be established in higher eukaryotes.

Pharmacological disruption of the MTDH-SND1 complex enhances tumor antigen presentation and synergizes with anti-PD-1 therapy in metastatic breast cancer.

Despite increased overall survival rates, curative options for metastatic breast cancer remain limited. We have previously shown that metadherin (MTDH) is frequently overexpressed in poor prognosis breast cancer, where it promotes metastasis and therapy resistance through its interaction with staphylococcal nuclease domain-containing 1 (SND1). Through genetic and pharmacological targeting of the MTDH-SND1 interaction, we reveal a key role for this complex in suppressing antitumor T cell responses in breast cancer.

Small-molecule inhibitors that disrupt the MTDH-SND1 complex suppress breast cancer progression and metastasis.

Metastatic breast cancer is a leading health burden worldwide. Previous studies have shown that metadherin (MTDH) promotes breast cancer initiation, metastasis and therapy resistance; however, the therapeutic potential of targeting MTDH remains largely unexplored. Here, we used genetically modified mice and demonstrate that genetic ablation of Mtdh inhibits breast cancer development through disrupting the interaction with staphylococcal nuclease domain-containing 1 (SND1), which is required to sustain breast cancer progression in established tumors.

Epithelial X-Box Binding Protein 1 Coordinates Tumor Protein p53-Driven DNA Damage Responses and Suppression of Intestinal Carcinogenesis.

Background & Aims: Throughout life, the intestinal epithelium undergoes constant self-renewal from intestinal stem cells. Together with genotoxic stressors and failing DNA repair, this self-renewal causes susceptibility toward malignant transformation. X-box binding protein 1 (XBP1) is a stress sensor involved in the unfolded protein response (UPR).

The predictive value of regulatory T cells on glucocorticoid sensitivity in patients with immune thrombocytopenia: a multicentre, prospective clinical study.

Glucocorticoids (GC) are used as the first-line treatment of immune thrombocytopenia (ITP), but 10-20% of patients are insensitive to them. Regulatory T cells (Tregs) can maintain immune tolerance in autoimmune diseases. The present research pooled 55 patients with newly diagnosed ITP and 44 healthy volunteers from seven hospitals.

Systemic risk factors correlated with hyperhomocysteinemia for specific genotypes and sex in the Chinese population.

Background: Methyltetrahydrofolate reductase (MTHFR) is a main regulatory enzyme in homocysteine (Hcy) metabolism. A common C677T mutation in the MTHFR gene results in decreased enzyme activity, which contributes to hyperhomocysteinemia (HHcy). Previous studies have shown that HHcy was correlated with various systemic diseases, such as cardiovascular disease, stroke, cancer, renal failure and so on.

Therapeutic Targeting of Metadherin Suppresses Colorectal and Lung Cancer Progression and Metastasis.

Colorectal and lung cancers account for one-third of all cancer-related deaths worldwide. Previous studies suggested that metadherin (MTDH) is involved in the development of colorectal and lung cancers. However, how MTDH regulates the pathogenesis of these cancers remains largely unknown.

Band Gaps and Transmission Characteristics Analysis on a Two-Dimensional Multiple-Scatter Phononic Crystal Structure.

In this paper, a novel wrap-around multi-scattering phononic crystal (PC) structure is proposed. Band gaps (BGs) and transmission characteristics of the present structure are calculated using finite element method (FEM). Through the calculations of single-scattering prototype, three complete BGs which are exhibited at low frequency and the fourth wide BG at high frequency are discovered.