HUPO BPP pilot study: a proteomics analysis of the mouse brain of different developmental stages.

This study is a part of the HUPO Brain Proteome Project (BPP) pilot study, which aims at obtaining a reliable database of mouse brain proteome, at the comparison of techniques, laboratories, and approaches as well as at preparing subsequent proteome studies of neurologic diseases. The C57/Bl6 mouse brains of three developmental stages at embryonic day 16 (E16), postnatal day 7 (P7), and 8 wk (P56) (n = 5 in each group) were provided by the HUPO BPP executive committee. The whole brain proteins of each animal were individually prepared using 2-DE coupled with PDQuest software analysis.

Selection and optimization procedure of synbiotic for cholesterol removal.

A selection and optimization procedure for the synbiotic combination of probiotic and prebiotics was established to optimize its cholesterol removal in vitro. In light of fermentability, prebiotics utilization by probiotics was highly variable and interspecies differences existed. Based on the results of fermentability, L.

Effect of Lactobacillus plantarum enteral feeding on the gut permeability and septic complications in the patients with acute pancreatitis.

Objective: To study the effect of the Lactobacillus plantarum (LP) enteral feeding on the gut permeability and sepsis in the patients with acute pancreatitis.

Subjects: Seventy-six subjects who stayed over 1 week in the hospital completed the study. Subjects were not treated with any lactobacillus supplement before the intervention.

Parathyroid hormone-related protein and calcium regulation in vitamin D-deficient sea bream (Sparus auratus).

Gilthead sea bream (Sparus auratus L.) were fed a vitamin D-deficient diet for 22 weeks. Growth rate, whole body mineral pools and calcium balance were determined.

[Effect of Wenmaitong on adhesion of monocytes to vascular endothelial cell induced by hyperlipidemic serum].

Objective: To study the effect of Wenmaitong (WMT) and its disassembled formulas on the adhesion of monocytes to endothelial cells induced by hyperlipidemic serum to explore the mechanism of WMT on early arteriosclerosis obliterans (ASO).

Methods: Serums containing whole WMT and its disassembled formulas, including the formula consisted of warming Jing and boosting qi part (Wenjin Yiqi, WY) and that of promoting blood circulation part (Huoxue Tongmai, HT), as well as the serum contained high concentration of lipids were prepared conventionally, respectively. The adhesion of monocytes cell strain THP-1 to human umbilical vascular endothelial cells (HUVEC) was determined by rose bengal stain method, and ELISA was used to detect expressions of intercellular adhesion molecule (ICAM-1), vascular cellular adhesion molecule (VCAM-1) and P-selectin on HUVEC surface.

PTHrP regulation and calcium balance in sea bream (Sparus auratus L.) under calcium constraint.

Juvenile gilthead sea bream were exposed to diluted seawater (2.5 per thousand salinity; DSW) for 3 h or, in a second experiment, acclimated to DSW and fed a control or calcium-deficient diet for 30 days. Branchial Ca2+ influx, drinking rate and plasma calcium levels were assessed.

Endothelin and calciotropic hormones share regulatory pathways in multidrug resistance protein 2-mediated transport.

The kidney of vertebrates plays a key role in excretion of endogenous waste products and xenobiotics. Active secretion in the proximal nephron is at the basis of this excretion, mediated by carrier proteins including multidrug resistance protein 2 (Mrp2). We previously showed that Mrp2 function is reduced by endothelin-1 (ET-1) through a basolateral B-type receptor, nitric oxide (NO), cGMP, and PKC (Notenboom S, Miller DS, Smits P, Russel FGM, Masereeuw R.

Influences of enteral nutrition combined with probiotics on gut microflora and barrier function of rats with abdominal infection.

Aim: To investigate the influences of enteral, parenteral nutrition and probiotics delivered by gut on intestinal microecology, epithelial tight junctions, immune and barrier function of rats with abdominal infection.

Methods: Rat abdominal infection models established with cecal ligation and perforation method, were divided into three groups: parenteral nutrition (PN group, n = 7), PN+enteral nutrition (EN group, n = 7) and PN + EN + probiotics (probiotics group, n = 7) via the needle jejunostomy and neck vein for five days. The total nutritional supplement of the three groups was isonitrogenic and isocaloric.

PTHrP potentiating estradiol-induced vitellogenesis in sea bream (Sparus auratus, L.).

In fish, vitellogenin is an important nutritional precursor protein produced solely in the liver and released into the blood where it binds calcium. In the gilthead sea bream (Sparus auratus) 17beta-Estradiol (E2) plays an important role in the synthesis of vitellogenin, but also the pituitary hormones prolactin (PRL) and growth hormone (GH) can stimulate vitellogenin induction in fish. Considering the emerging involvement of PTHrP in fish calcium metabolism and the importance of calcium regulation in reproduction, we investigated the possible role of PTHrP in vitellogenesis.

[Construction of standard human transcript dataset based on RefSeq and human genome sequence database].

The NCBI Reference Sequence (RefSeq) database aimed to provide a biologically non-redundant collection of DNA, RNA, and protein sequences and to promote the research on genes and proteins of human beings and other species. However, because of widely distributed polymorphisms and different quality control of experiments in individual laboratories, there are potential problems need to be identified in the RefSeq database. Regarding which, we herein define the concept, standard transcript, based on the Central Dogmas of Biology that each standard transcript should be perfectly mapped to the standard genomic DNA sequence at the exon level.

Methyl bromide as a quarantine treatment for Anoplophora glabripennis (Coleoptera: Cerambycidae) in regulated wood packing material.

Anoplophora glabripennis (Motschulsky), has recently (since 1996) established in limited infestations near several cities in the United States. A. glabripennis was probably introduced into the United States with solid wood packing or dunnage.

[An in vitro study of cholesterol-lowering properties of probiotics isolated from the human feces].

21 strains of Lactobacillus and Bifidobacterium, isolated from feces of healthy youth and children feces and identified by molecular biological methods, together with 6 strains of probiotics preserved in Onlly lab were studied in the experiments, including removal cholesterol from media, bile-tolerance and acid-tolerance. The results demonstrated that all strains could remove cholesterol from media and removal rates of 5 strains were more than 40%. Meanwhile these 5 strains had high removal effectiveness.

[Analysis of the probiotic Bifidobacterium and Lactobacillus community in child intestinal flora].

To investigate the distribution of child intestinal flora and the composition of its key probiotics community, study on intestinal flora of 21 Chinese children (age 2 - 5) was conducted, which included bacteria isolation and counting, 16S rDNA sequencing and homology analysis. For identification of the key probiotics such as Bifidobacterium and Lactobacillus in children feces at the species level, the specific primers Im26/Im3 and L159/L677 for PCR amplification of partial 16S rDNA were used. The results show that the composition of child intestinal flora is was relatively stable and almost same to the intestinal flora of the youth (age 20 - 25).

[Randomized clinical study comparing gemcitabine and oxaliplatin versus gemcitabine and cisplatin for advanced non-small cell lung cancer in elderly patients].

Background: In most instances, advanced non-small cell lung cancer (NSCLC) is treated with primary chemotherapy. Many chemotherapy regimens can palliate cancer-related symptoms. Quality of life and modestly improved survival are very important especially for elderly patients.

Stanniocalcin in the euryhaline flounder (Platichthys flesus): primary structure, tissue distribution, and response to altered salinity.

Stanniocalcin (STC) is a homodimeric glycoprotein hormone that was first discovered in fish, where it is largely produced by a unique endocrine gland, the corpuscles of Stannius (CS). In bony fish, it is thought to be an important regulator of calcium and phosphate uptake from the aquatic environment. This report describes the molecular cloning of STC from euryhaline flounder (Platichthys flesus) CS cDNA and genomic DNA.

Production and characterisation of gilthead sea bream (Sparus auratus) recombinant parathyroid hormone related protein.

The production and purification of gilthead sea bream recombinant parathyroid hormone related protein [sbPTHrP(1-125)] using an Escherichia coli system and one step purification process with continuous elution gel electrophoresis is reported. The cDNA encoding sbPTHrP(1-125) was cloned into a prokaryotic expression vector pET-11a. The recombinant plasmid was used to transfect E.

Measurement of PTHrP, PTHR1, and CaSR expression levels in tissues of sea bream (Sparus aurata) using quantitative PCR.

A quantitative PCR (Q-PCR) method has been established to measure the mRNA expression levels of parathyroid hormone-related protein (PTHrP), parathyroid hormone receptor type 1 (PTHR1), and calcium-sensing receptor (CaSR) in sea bream (Sparus aurata), using the housekeeping gene, beta-actin, as endogenous control. TaqMan primers and probes were designed using the Primer Express program, according to the published/unpublished sequences of the three target genes and beta-actin of sea bream. Different tissues including gill, kidney, duodenum, hindgut, rectum, liver, heart, brain, pituitary, skin, muscle, and gonad were removed and immediately snap-frozen from three juvenile sea bream (100-150 g) cultured in sea water.

Effect of lactobacillus on the gut microflora and barrier function of the rats with abdominal infection.

Aim: To investigate the effect of probiotics supplemented by gut on the tight junctions of epithelial cells, barrier function and the microflora of rats with abdominal infection.

Methods: After the model of cecal ligation and perforation established, SD rats were divided into two groups: parenteral nutrition (PN) group and PN+probiotics (probiotics) group, PN solution was supplemented by neck vein and probiotics was delivered via the jejunostomy tube for five days. Vena cava blood and the homogenated tissue of liver, lung and mesenteric lymph nodes were cultured to determine the bacterial translocation rate (BTR).

Combination of pulsed-wave Doppler and real-time three-dimensional color Doppler echocardiography for quantifying the stroke volume in the left ventricular outflow tract.

Real-time three-dimensional (3-D) color Doppler echocardiography (RT3D) is capable of quantifying flow. However, low temporal resolution limits its application to stroke volume (SV) measurements. The aim of the present study was, therefore, to develop a reliable method to quantify SV.

A geometric deformable model for echocardiographic image segmentation.

Gradient vector flow (GVF), an elegant external force for parametric deformable models, can capture object boundaries from both sides. A new geometric deformable model is proposed that combines GVF and the geodesic active contour model. The level set method is used as the numerical method of this model.

Compression of echocardiographic scan line data using wavelet packet transform.

An efficient compression strategy is indispensable for digital echocardiography. Previous work has suggested improved results utilizing wavelet transforms in the compression of 2D echocardiographic images. Set partitioning in hierarchical trees (SPIHT) was modified to compress echocardiographic scanline data based on the wavelet packet transform.

Impact of temporal resolution on flow quantification by real-time 3D color Doppler echocardiography: numerical modeling and animal validation study.

Real-time, 3D color Doppler echocardiography (RT3D) is capable of quantifying flow at the LV outflow tract (LVOT). However, previous works have found significant underestimation for flow rate estimation due to finite scanning time (ST) of the color Doppler. The authors have, therefore, developed a mathematical model to correct the impact of ST on flow quantification and validated it by an animal study.

Compression of real time volumetric echocardiographic data using modified SPIHT based on the three-dimensional wavelet packet transform.

Real-time three-dimensional echocardiography has been introduced to provide improved quantification and description of cardiac function. Data compression is desired to allow efficient storage and improve data transmission. Previous work has suggested improved results utilizing wavelet transforms in the compression of medical data including 2D echocardiogram.