A universal mechanism on desiccation tolerance of Cronobacter based on intracellular trehalose accumulation regulated by EnvZ/OmpR.

Cronobacter (seven species) can survive in dry powdered infant formula for a long time, but the thorough molecular mechanism of resistance to desiccation remains elusive. Here we examine the regulation mechanism of Cronobacter's tolerance to desiccation by the typical two-component system (TCS) EnvZ/OmpR. When exposed to desiccation conditions, Cronobacter showed higher survival than other pathogens, as well as significantly up-regulated expression of ompR and otsAB genes with markedly decreased survival of their mutants, suggesting their relationship with desiccation tolerance.

Genome Characterization of the Novel Lytic Enterobacter cloacae Phage vB_EclM_Q7622.

Enterobacter cloacae is a widespread opportunistic pathogen that causes urinary tract infection. The abuse of antibiotics enabled multidrug-resistant strains to spread. Bacteriophage therapy is a naturally, safe, and efficient alternative treatment technology against multi-resistant bacteria.

Characterization and Genomic Analysis of Novel Phage vB_VpaP_DE10.

In the present study, a novel lytic phage, vB_VpaP_DE10, was isolated from sewage samples collected in Guangzhou city, China. Transmission electron microscopy revealed that phage vB_VpaP_DE10 has an icosahedral head (52.4 ± 2.

Characterization of the Novel Phage vB_VpaP_FE11 and Its Potential Role in Controlling Biofilms.

causes aquatic vibriosis. Its biofilm protects it from antibiotics; therefore, a new different method is needed to control for food safety. Phage therapy represents an alternative strategy to control biofilms.

Characterization and genome analysis of a novel Vibrio parahaemolyticus phage vB_VpP_DE17.

A novel phage vB_VpP_DE17, which infects Vibrio parahaemolyticus, was isolated from the sewer of the Huangsha aquatic market in Guangzhou. Transmission electron microscopy indicated that DE17 had an icosahedral head (47 ± 2 nm diameter) and a short, non-contractile tail (17 ± 2 nm). The genome of DE17 was a double-stranded linear DNA with a length of 43,397 bp and GC content of 49.

Isolation and Characterization of a Novel Phage vB_SalP_TR2.

is a widely distributed foodborne pathogen. The use of phages as biocontrol agents has recently gained significant interest. Because the genus has high diversity, efforts are necessary to identify lytic phages focusing on different serovars.

Isolation and Characterization of the Novel Phages vB_VpS_BA3 and vB_VpS_CA8 for Lysing .

Accumulating evidence has indicated that the multiple drug resistant may pose a serious threat to public health and economic concerns for humans globally. Here, two lytic bacteriophages, namely vB_VpS_BA3 and vB_VpS_CA8, were isolated from sewage collected in Guangzhou, China. Electron microscopy studies revealed both virions taxonomically belonged to the family with icosahedral head and a long non-contractile tail.

Genome characterization of the novel lytic Vibrio parahaemolyticus phage vB_VpP_BA6.

A lytic bacteriophage, designated Vibrio phage vB_VpP_BA6, was isolated from sewage collected in Guangzhou, China. The double-stranded DNA genome of phage BA6 is composed of 50,520 bp with a G+C content of 41.77%.

[Evaluation of white matter myelination in preterm infants using DTI and MRI].

Objective: To investigate the features of white matter myelin development in preterm infants using magnetic resonance imaging (MRI) and diffusion tensor imaging (DTI).

Methods: A total of 31 preterm infants with a gestational age of ≤32 weeks and a birth weight of <1 500 g were enrolled. According to head MRI findings, these infants were divided into preterm group with brain injury (12 infants) and preterm group without brain injury (19 infants).

Gadolinium-enhanced magnetic resonance imaging of the knee: an experimental approach.

Purpose: The purpose of this study was to examine gadolinium-enhanced magnetic resonance imaging (MRI) for monitoring cartilage degeneration.

Methods: This is a proof-of-concept study in an animal model. Adult New Zealand rabbits were randomly stratified into five groups.