Core-Shell Gold Nanoparticles@Pd-Loaded Covalent Organic Framework for In Situ Surface-Enhanced Raman Spectroscopy Monitoring of Catalytic Reactions.

A core-shell nanostructure of gold nanoparticles@covalent organic framework (COF) loaded with palladium nanoparticles (AuNPs@COF-PdNPs) was designed for the rapid monitoring of catalytic reactions with surface-enhanced Raman spectroscopy (SERS). The nanostructure was prepared by coating the COF layer on AuNPs and then in situ synthesizing PdNPs within the COF shell. With the respective SERS activity and catalytic performance of the AuNP core and COF-PdNPs shell, the nanostructure can be directly used in the SERS study of the catalytic reaction processes.

Sirtuin 2 up-regulation suppresses the anti-tumour activity of exhausted natural killer cells in mesenteric lymph nodes in murine colorectal carcinoma.

Natural killer (NK) cells inhibit colorectal carcinoma (CRC) initiation and progression through their tumoricidal activity. However, cumulative evidence suggests that NK cells become functionally exhausted in patients with CRC. To deepen the understanding of the mechanisms underlying CRC-associated NK cell exhaustion, we explored the expression and effect of Sirtuin 2 (Sirt2) in mesenteric lymph node (mLN) NK cells in a murine colitis-associated CRC model.

Cell membrane-targeted surface enhanced Raman scattering nanoprobes for the monitoring of hydrogen sulfide secreted from living cells.

Hydrogen sulfide (HS), an important gas signal molecule, participates in intercellular signal transmission and plays a considerable role in physiology and pathology. However, in-situ monitoring of HS level during the processes of material transport between cells remains considerably challenging. Herein, a cell membrane-targeted surface-enhanced Raman scattering (SERS) nanoprobe was designed to quantitatively detect HS secreted from living cells.

Schiff Base Reaction in a Living Cell: In Situ Synthesis of a Hollow Covalent Organic Polymer To Regulate Biological Functions.

Artificially performing chemical reactions in living biosystems to attain various physiological aims remains an intriguing but very challenging task. In this study, the Schiff base reaction was conducted in cells using Sc(OTf) as a catalyst, enabling the in situ synthesis of a hollow covalent organic polymer (HCOP) without external stimuli. The reversible Schiff base reaction mediated intracellular Oswald ripening endows the HCOP with a spherical, hollow porous structure and a large specific surface area.

Smart Nanoplatform for Visualizing Hydrogen Sulfide and Amplifying Oxidative Stress to Tumor Apoptosis.

Oxidative stress is involved in various signaling pathways and serves a key role in inducing cell apoptosis. Therefore, it is significant to monitor oxidative stress upon drug release for the assessment of therapeutic effects in cancer cells. Herein, a glutathione (GSH)-responsive surface-enhanced Raman scattering (SERS) nanoplatform is proposed for ultra-sensitively monitoring the substance related with oxidative stress (hydrogen sulfide, HS), depleting reactive sulfur species and releasing anticancer drugs to amplify oxidative stress for tumor apoptosis.

Dual-Modal Apoptosis Assay Enabling Dynamic Visualization of ATP and Reactive Oxygen Species in Living Cells.

ATP and reactive oxygen species (ROS) are considered significant indicators of cell apoptosis. However, visualizing the interplay between apoptosis-related ATP and ROS is challenging. Herein, we developed a metal-organic framework (MOF)-based nanoprobe for an apoptosis assay using duplex imaging of cellular ATP and ROS.

Self-Assembled Plasmonic Nanojunctions Mediated by Host-Guest Interaction for Ultrasensitive Dual-Mode Detection of Cholesterol.

Herein, a fluorescence and surface-enhanced Raman spectroscopy dual-mode system was designed for cholesterol detection based on self-assembled plasmonic nanojunctions mediated by the competition of rhodamine 6G (R6G) and cholesterol with β-cyclodextrin modified on gold nanoparticles (HS-β-CD@Au). The fluorescence of R6G was quenched by HS-β-CD@Au due to the fluorescence resonance energy transfer effect. When cholesterol was introduced as the competitive guest, R6G in the cavities of HS-β-CD@Au was displaced to recover its fluorescence.

AuNPs-COFs Core-Shell Reversible SERS Nanosensor for Monitoring Intracellular Redox Dynamics.

The redox homeostasis in living cells is greatly crucial for maintaining the redox biological function, whereas accurate and dynamic detection of intracellular redox states still remains challenging. Herein, a reversible surface-enhanced Raman scattering (SERS) nanosensor based on covalent organic frameworks (COFs) was prepared to dynamically monitor the redox processes in living cells. The nanosensor was fabricated by modifying the redox-responsive Raman reporter molecule, 2-Mercaptobenzoquione (2-MBQ), on the surface of gold nanoparticles (AuNPs), followed by the in situ coating of COFs shell.

Methods for characterizing the structure of starch in relation to its applications: a comprehensive review.

Starch is a major part of the human diet and an important material for industrial utilization. The structure of starch granules is the subject of intensive research because it determines functionality, and hence suitability for specific applications. Starch granules are made up of a hierarchy of complex structural elements, from lamellae and amorphous regions to blocklets, growth rings and granules, which increase in scale from nanometers to microns.

Down-regulation of PR/SET domain 10 underlies natural killer cell dysfunction in hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) is the world's leading cause of tumor-related mortalities. Natural killer (NK) cells play a critical role at the first immunological defense line against HCC initiation and progression. NK cell dysfunction is therefore an important mechanism for immune evasion of HCC cells.

Mechanisms Underlying the Effect of Tea Extracts on Digestion of Wheat Starch.

The effect of extracts from four types of tea made from (green, white, black, and oolong) on amylolysis of gelatinized starch and the underlying mechanisms were studied. Of the four extracts, black tea extract (BTE) gave the strongest inhibition of starch digestion and on α-amylase activity. Fluorescence quenching and surface plasmon resonance (SPR) showed compounds in BTE bound to α-amylase more strongly than those in the green, white, and oolong tea extracts.

A novel affinity peptide-antibody sandwich electrochemical biosensor for PSA based on the signal amplification of MnO-functionalized covalent organic framework.

This work describes a novel affinity peptide-antibody sandwich electrochemical strategy for the ultrasensitive detection of prostate-specific antigen (PSA). Herein, polydopamine-coated boron-doped carbon nitride (Au@PDA@BCN) was synthesized and used as a sensing platform to anchor gold nanoparticles and immobilize primary antibody. Meanwhile, AuPt metallic nanoparticle and manganese dioxide (MnO)-functionalized covalent organic frameworks (AuPt@MnO@COF) was facilely synthesized to serve as a nanocatalyst and ordered nanopore for the enrichment and amplification of signal molecules (methylene blue, MB).

Ultrahigh stable lead halide perovskite nanocrystals as bright fluorescent label for the visualization of latent fingerprints.

Fingerprints formed by the raised papillary ridges are one of the most important markers for individual identification. However, the current visualization methods for latent fingerprints (LFPs) suffer from poor resolution, low contrast, and high toxicity. In this work, the CsPbBr/CsPbBrnanostructured composite crystal (CsPbBr/CsPbBrNCC) were synthesized via a simple chemical solvent-assisted method.

Revealing the mechanisms of starch amylolysis affected by tea catechins using surface plasmon resonance.

The interaction of polyphenolic catechins from Camellia sinensis tea with α-amylase, and the effects of the interactions on the kinetics of starch amylolysis, were investigated. Binding studies using surface plasmon resonance (SPR) and enzyme kinetic analysis indicated that the in vitro digestibility of gelatinized wheat starch was decreased by the addition of catechins present in tea. Tea catechins decreased enzyme activity by reducing the maximum velocity (V) of the α-amylase, but had little effect on the Michaelis-Menten constant (K).

Ultrasensitive electrochemical sensor for prostate specific antigen detection with a phosphorene platform and magnetic covalent organic framework signal amplifier.

Magnetic covalent organic frameworks (COFs) are useful mesoporous materials for the enrichment and separation of analytes, and are utilized in the pretreatment of samples. However, the use of magnetic COFs in electrochemical immunosensors has rarely reported. Herein, a novel electrochemical assay for the determination of prostate specific antigen (PSA) was developed using black phosphorene (BPene) as a platform and magnetic COFs for signal amplification.

[Experience of preventing bronchial asthma by blistering therapy of dog-day moxibustion].

Professor owns unique experience of preventing bronchial asthma by the blistering therapy of dog-day moxibustion. He believes that the method has the action of festering moxibustion without its adverse reaction, the pathogenesis of asthma is the impairment of the dispersing and descending of the lung. When the lung,the spleen and the kidney are deficient,pathogenic factors,such as phlegm,damp and the cold,twist in the lung and the air passage is blocked, then asthma happens.

[Professor SONG Nanchang's experience for treatment of peripheral facial paralysis].

Professor SONG Nanchang's clinical experience and characteristics for treatment of peripheral facial paralysis are introduced. In clinical treatment, professor SONG has adopted staging treatment strategy, and performed acupuncture stimulation with different levels. He attaches great importance to the acupoint selection on distal limbs.

[Comparative study on effect of acupoint heat-sensitive moxibustion and Seretide on the symptoms of bronchial asthma at chronic persistent stage].

Objective: To observe the difference in the efficacy on the symptoms of bronchial asthma at the chronic persistent stage between acupoint heat-sensitive moxibustion and western medicine with Seretide.

Methods: Sixty-four cases were randomly divided into a heat-sensitive moxibustion group (32 cases) and a western medication group (32 cases). In the heat-sensitive moxibustion group, the sensitized points located between Feishu (BL 13) and Geshu (BL 17) or in the region 6-cun lateral from the 1st and the 2nd intercostal spaces of the chest were selected.

[Observation on therapeutic effect of muscular needling combined with scarring moxibustion on active stage of rheumatoid arthritis].

Objective: To observe the therapeutic effect of muscular needling combined with scarring moxibustion on active stage of rheumatoid arthritis (RA).

Methods: Sixty cases of RA were randomly divided into a muscular needling group and a medication group, 30 cases in each group. The muscular needling group was treated by muscular needling on Quchi (LI 11), Sanyinjiao (SP 6), etc.