Genotype diversity, phylogenetic analysis and seasonality of isolates of Acanthamoeba spp. in swimming pools in Kafrelsheikh, Egypt.

Species of Acanthamoeba Volkonsky, 1931 are the commonest among free-living amoebae that are widespread in different water resources but with lacking phylogenetic data. This study aims at detecting molecular prevalence and genetic diversity of Acanthamoeba isolates in Kafrelsheikh Governorate, Egypt. Forty-eight water samples were collected from 12 swimming pools; four samples during each season over one year.

In vitro amoebicidal effect of ethanol extract and honey against . cysts.

This study evaluated in vitro effect of different concentrations of () ethanol extract and honey against spp. cysts in comparison with chlorhexidine (the drug of choice for treatment of infection) at different incubation periods. Four different concentrations of the tested agents were used, 100, 200, 400, and 600 μg/ml for and 25, 50, 100, and 200 μg/ml for honey.