In vitro study on role of Hsp70 expression in DNA damage of human embryonic lung cells exposed to Benzo[a]pyrene.

Objective: Benzo[a]pyrene (B[a]P), a ubiquitous environmental pollutant, is a potent procarcinogen and mutagen that can elicit tumors, leading to malignancy. Heat shock proteins (Hsp) have been shown to protect cells against damages caused by various stresses including exposure to numerous chemicals. Whether Hsps, or more specifically Hsp70, are involved in repair of B[a]P-induced DNA damage is currently unknown.

[Effects of mitogen activated protein kinase signal transduction pathways on heat shock protein 70 gene expression in endothelial cells exposed to benzo(a)pryene].

Objective: To investigate the effects of mitogen activated protein kinase (MAPK) signal transduction pathways on heat shock protein 70 (HSP70) gene expression in endothelial cells exposed to benzo(a)pryene (BaP).

Methods: Porcine aortic endothelial cells were pre-treated or by PD98059 (10 micro mol/L) or SB203580 (20 micro mol/L) for 1 hour, then treated with different concentrations of BaP (0, 0.1, 0.

[RT-PCR method for detecting the expression of transforming growth factor-beta1 gene in lung tissues of silica-treated mice].

Objective: To investigate the gene expression of transforming growth factor-beta(1) (TGF-beta(1)) in lung tissues of silica-treated mice.

Methods: The experimental mice were divided into control and silica group. 0.

[Immunohistochemical method for the detecting expression of transforming growth factor-beta1 in lung tissues of silica-treated mice].

Objective: To investigate the protein expression of transforming growth factor-beta(1) (TGF-beta(1)) in lung tissues of silica-treated mice.

Methods: The experimental mice were divided into control and silica groups. 0.

[The expression of cytochrome P450 1A1 of endothelial cells induced by benzo(a)pyrene].

Objective: To explore the effect of benzo(a)pyrene (BaP) on the expression and the activities of cytochrome P450 1A1 (CYP1A1) of porcine aortic endothelial cells.

Methods: Porcine aortic endothelial cells were cultured in vitro, and treated with different concentrations of BaP (0, 0.5, 1.