Down-regulation of histone deacetylase 7 reduces biological activities of retinal microvascular endothelial cells under high glucose condition and related mechanism.

Aim: To investigate the expression and effect of histone deacetylase 7 (HDAC7) in human retinal microvascular endothelial cells (HRMECs) under high glucose condition and related mechanism, and the expression of HDAC7 in the retinal tissue in diabetic rats.

Methods: The expression of HDAC7 in HRMECs under high glucose and the retinal tissue from normal or diabetic rats were detected with immunohistochemistry and Western blot. LV-shHDAC7 HRMECs were used to study the effect of HDAC7 on cell activities.

Neuroprotective effect of engineered butyricum-pMTL007-GLP-1 on Parkinson's disease mice models via promoting mitophagy.

Parkinson's disease (PD) is a common neurodegenerative disease with limited treatment and no cure, hence, broadening PD drug spectrum is of great significance. At present, engineered microorganisms are attracting increasing attention. In this study, we constructed an engineered strain of -GLP-1, a (a probiotic) that consistently expresses glucagon-like peptide-1 (GLP-1, a peptide-based hormone with neurological advantage) in anticipation of its use in PD treatment.

Palladium-catalyzed monoselective C-H borylation of acetanilides under acidic conditions.

A practical Pd-catalyzed reaction was developed to achieve C-H activation/C-B cross-coupling of acetanilides under acidic conditions. The new reaction shows a good functional group tolerance and an exclusive mono-selectivity. This C-H borylation method may provide a generally applicable route for the conversion of C-H moieties into many other types of bonds.

Studies on specific interaction of beta-2-glycoprotein I with HBsAg.

Aim: To observe the binding activity of beta-2-glycoprotein I(beta(2)GPI) to hepatitis B surface antigen (HBsAg) and the possible roles of beta(2)GPI in hepatitis B virus (HBV) infection.

Methods: The rationale of ELISA methods and ELISA-based research method and ligand-blotting technique were used to detect the specific interaction of beta(2)GPI with HBsAg.

Results: With the increase of rHBsAg, the binding of beta(2)GPI to rHBsAg elevated, and these changes had statistic significance.

Effects of the Superoxide Anionic Radicals to the Cultured Cerebral Cortex Neuron of Newborn Rat.

The effect of the superoxide anionic radicals to the cultured cerebral cortex neuron of newborn rat was investigated by using xanthine oxidase/xanthine. The results are as follows. The growth of neuron was inhibited ATPase activity decreased to 4.