Soil microbial metabolism is vital for nutrient cycling and stability of an ecosystem. To elucidate the long-term effects of biochar application on nutrient limitations and carbon use efficiency (CUE) of soil microbial metabolisms, biochars pyrolyzed at 450℃ from trunks and branches of fruit trees under an oxygen-limited condition were mixed with the top Lou soils (0-20 cm) with application amounts of 0, 20, 40, 60, and 80 t·hm in 2012. Corn-wheat rotation was carried out afterwards for seven years.
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