[Not Available].

Objective: To study the impact of anticoagulant to the quality of umbilical cord blood (UCB).

Methods: 6060 cord blood units (CBUs) were classified into five groups, such as 28 ml: (10-29) ml, 28 ml: (30-69) ml, 28 ml: (70-109) ml, 28 ml: (110-150) ml and 28 ml: (>150) ml according to volume ratio of anticoagulant and CBVs. The count of pre-cryopreservation total nucleated cell (pre-TNC), the viability of nucleated cell (VNC), the amount of CFU-GM and the ratio changes of CD34 were evaluated and analyzed statistically.

[Biological characteristics of mesenchymal stem cell and hematopoietic stem cell in the co-culture system].

The aim of the present study was to obtain the qualified hematopoietic stem/progenitor cells (HSC/HPC) and human umbilical cord-mesenchymal stem cells (MSC) in vitro in the co-culture system. Cord blood mononuclear cells were separated from umbilical cord blood by Ficoll lymphocyte separation medium, and then CD34 HSC was collected by MACS immunomagnetic beads. The selected CD34 HSC/HPC and MSC were transferred into culture flask.