ERB-041, a selective ER beta agonist, inhibits iNOS production in LPS-activated peritoneal macrophages of endometriosis via suppression of NF-kappaB activation.

Objective: The aim of the present study was to assess the anti-inflammatory effects of selective ER beta (ER beta) agonist on lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) production in peritoneal macrophages (PMs) of endometriosis (EMS).

Methods: ER alpha (ER alpha) and ER beta expressions in PMs were analyzed by RT-PCR and immunoblot. The PMs of endometriosis were exposed to increasing concentrations of ER beta agonist ERB-041 over a period from 0.

NF-kappaB decoy oligonucleotides suppress RANTES expression and monocyte chemotactic activity via NF-kappaB inactivation in stromal cells of ectopic endometrium.

Background: The nuclear factor kappa B (NF-kappaB) pathway is a critical mediator of regulated on activation, normal T cell expressed and secreted (RANTES) gene regulation and therefore represents a potential target for therapy of endometriosis-associated symptoms.

Objective: The objective of this study was to investigate the effect of NF-kappaB decoy oligonucleotides (ODNs) on NF-kappaB activation, RANTES expression, and monocyte chemotactic activity in ectopic endometrial stromal cells in vitro.

Methods: A specific sandwich enzyme-linked immunosorbent assay (ELISA) was used to quantify RANTES expression in ectopic and normal endometrial stromal cells stimulated by interleukin (IL)-1beta.

Ectopic, autologous eutopic and normal endometrial stromal cells have altered expression and chemotactic activity of RANTES.

Objective: To evaluate if the expression and chemotactic activity of RANTES are different in IL-1beta treated autologous eutopic endometrial stromal cells compared to ectopic and normal endometrium.

Study Design: Conditioned media from IL-1beta-treated ectopic, autologous eutopic and normal endometrial stromal cells were analyzed with a specific sandwich ELISA to quantify RANTES. The monocyte chemotactic activity of RANTES was assayed in a Boyden Chamber.

[Influence of nuclear factor-kappaB decoy oligonucleotides on RANTES expression and monocyte chemotactic activity in stromal cells of ectopic endometrium].

Objective: To study the inhibitory effect on the expression of regulated upon activation, normal T cell expressed and secreted (RANTES) and monocyte chemotactic activity of ectopic endometrial stromal cells by nuclear factor (NF)-kappaB decoy oligonucleotides (ODN).

Methods: The stromal cells of ectopic endometrium were divided into 3 groups. Two groups were cultured with or without 10 microg/L of interleukin (IL)-1beta.

On-line sample digestion using an electromagnetic heating column for the determination of zinc and manganese in tea leaf by flame atomic absorption spectrometry.

A new on-line sample digestion system using electromagnetic induction heating technique was developed for the determination of zinc and manganese in tea leaf by flame atomic absorption spectrometry (FAAS). The homemade electromagnetic heating column (EMHC), whose effective diameter was about 1mm, was composed of a polytetrafluoroethylene (PTFE) outer tube and seven compactly packed PTFE coil-coated iron wires. The pre-digested sample solution was pumped through EMHC and then transferred directly to FAAS for determination.

Combined treatment of ionizing radiation with genistein on cervical cancer HeLa cells.

The anticancer agent genistein inhibits cell growth of tumor cell lines from various malignancies. In our study, we investigated the effectiveness of combined treatment of ionizing radiation (IR) with genistein on cervical HeLa cells and its possible mechanism. It was found that the inhibitory rate in cells with combined treatment was significantly higher than that of the cells treated with IR or genistein alone.