Publications by authors named "Han Seul Lee"

Chinese hamster ovary (CHO)-K1 cells are widely used in biomedical research relevant to cancer, toxicity screening, and viruses, as well as in the production of recombinant proteins for biopharmaceuticals. In this study, liquid chromatography (LC)-electrospray ionization (ESI)-higher energy collisional dissociation (HCD)-tandem mass spectrometry (MS/MS) was used to characterize the surface and lysate N-glycans of CHO-K1 cells and analyze their structures. The relative quantity (%) of each N-glycan and absolute quantity (pmol) of total N-glycans were also obtained.

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Citronellol is widely utilized in consumer products, including cosmetics, fragrances, and household items. However, despite being considered a relatively safe chemical, the health effects and toxicity mechanisms associated with exposure to high concentrations of citronellol, based on product content, remain inadequately understood. Here, we aimed to analyze the neurological effects of citronellol in zebrafish larvae using behavioral and histological analyses and elucidate the mechanisms underlying its neurotoxicity in vivo.

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Phosvitin (PV), a highly phosphorylated protein found in chicken egg yolk, possesses multiple bioactivities (including anti-aging and anticancer) and functional properties (including emulsifier and metal-binding capacities). The carbohydrate moiety attached to PV has been reported, but its N-glycan structure is unknown. In this study, we performed structural and quantitative analyses of N-glycans from PV using liquid chromatography-tandem mass spectrometry (MS/MS).

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Article Synopsis
  • MIN6, a mouse pancreatic beta cell line, is crucial for diabetes research, focusing on the role of N-glycoproteins in insulin secretion metabolism.
  • The study identified and analyzed 50 different N-glycans in MIN6 cell membranes using advanced mass spectrometry, highlighting their relative abundances and structural categories, including bisecting N-acetylglucosamine, core-fucosylated, and sialylated types.
  • Findings indicate that these N-glycan structures significantly influence cell adhesion and interactions, potentially enhancing insulin secretion efficiency in beta cells, with notable differences compared to human islets.
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Host N-glycans play an essential role in the attachment, invasion, and infection processes of viruses, including zoonotic infectious diseases. The similarity of N-glycans in the trachea and lungs of humans and pigs facilitates the cross-species transmission of influenza viruses through respiratory tracts. In this study, the structure and quantity of N-glycans in the plasma of humans, pigs, and chickens were analyzed using liquid chromatography-quadrupole-Orbitrap-tandem mass spectrometry.

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Bovine intestinal alkaline phosphatase (biALP), a membrane-bound plasma metalloenzyme, maintains intestinal homeostasis, regulates duodenal surface pH, and protects against infections caused by pathogenic bacteria. The N-glycans of biALP regulate its enzymatic activity, protein folding, and thermostability, but their structures are not fully reported. In this study, the structures and quantities of the N-glycans of biALP were analyzed by liquid chromatography-electrospray ionization-high energy collision dissociation-tandem mass spectrometry.

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We have developed a fluorescent probe DBT-Cl ((E)-2-(2-(4-(diphenylamino)benzylidene) hydrazinyl)-N,N,N-trimethyl-2-oxoethan-1-aminium chloride) for ClO with an aggregation-induced emission (AIE) strategy depending on solvent polarity. DBT-Cl possessed a prominent solvatochromic emission property with intramolecular charge transfer (ICT) from the TPA (triphenylamine) to the amide group, which was studied by spectroscopic analysis and DFT calculations. These unique AIE properties of DBT-Cl led to the recognition of ClO with high fluorescent selectivity.

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Article Synopsis
  • - HAPLN1 is an extracellular matrix protein that helps stabilize connections between hyaluronan and proteoglycan, but its N-glycosylation (a type of carbohydrate modification) is not well understood.
  • - In this study, researchers used advanced techniques to identify 66 N-glycopeptides from HAPLN1 that had been expressed in CHO cells, finding significant differences in the types of N-glycans at two specific sites (Asn 6 and Asn 41).
  • - The findings suggest that the N-glycans at these sites serve different purposes: those at Asn 6 help protect the protein, while those at Asn 41 enhance its ability to interact
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Bovine testicular hyaluronidase (BTH), which accelerates the absorption and dispersion of drugs by decomposing hyaluronan in subcutaneous tissues, has been used in medical applications, including local anesthesia, ophthalmology, and dermatosurgery. The requirement of N-glycans for the activity of human hyaluronidase has been reported, and BTH has greater activity than human hyaluronidase. However, the N-glycan characteristics of BTH are unclear.

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  • Studies show that legacy PFAS (PFOS and PFOA) were tested for ocular toxicity in zebrafish larvae, revealing significant effects on visual behaviors compared to alternative PFAS (PFBS and PFBA).
  • Exposure to PFOS decreased phototactic responses in a concentration-dependent manner and affected optomotor responses, while PFOA's effects were limited to a higher concentration (100 mg/L).
  • Gene expression analyses confirmed that PFOS downregulated critical visual function genes, indicating that it poses the highest risk for visual toxicity among the substances tested, with alternatives showing no toxic effects.
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Trace element concentrations and isotope ratios of hair reflect the blood levels at the time of hair formation, but can be affected by external factors such as dyeing, bleaching, and bathing. To investigate the effect of dyeing, bleaching, and bathing on hair, hair was immersed in tap water, and changes in trace element concentrations and the Sr isotope ratio were observed over time. During soaking, alkaline earth metals (Ca, Mg, and Sr) from tap water were gradually absorbed into the hair over time.

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Natural variations of Sr/Sr ratios in biological samples, such as human hair, provide a biological record of provenance. Spatial distribution maps reflecting heterogeneity in isotopic signatures across large geographical regions are helpful for discerning the provenance and mobility of organisms. In this national-scale study conducted across South Korea, we investigated the spatial distribution patterns of Sr/Sr ratios in human hair and tap water samples to determine their spatial variabilities and the relationships of isotopic signatures between hair and tap water.

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Dietary homogenization has progressed worldwide due to westernization and the globalization of food production systems. We investigated dietary heterogeneity in South Korea by examining the spatial distribution of carbon (C), nitrogen (N), and sulfur (S) isotope ratios using 264 human hair samples. Overall, variation in isotope values was small, indicating low dietary heterogeneity.

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