Overexpression of MtIPT gene enhanced drought tolerance and delayed leaf senescence of creeping bentgrass (Agrostis stolonifera L.).

Background: Isopentenyltransferases (IPT) serve as crucial rate-limiting enzyme in cytokinin synthesis, playing a vital role in plant growth, development, and resistance to abiotic stress.

Results: Compared to the wild type, transgenic creeping bentgrass exhibited a slower growth rate, heightened drought tolerance, and improved shade tolerance attributed to delayed leaf senescence. Additionally, transgenic plants showed significant increases in antioxidant enzyme levels, chlorophyll content, and soluble sugars.

The Effect of Ethephon on Ethylene and Chlorophyll in Leaves.

( Steud.) is a kind of warm-season turfgrass with many excellent characteristics. However, the shorter green period and longer dormancy caused by cold stress in late autumn and winter are the most limiting factors affecting its application.

Differential Regulations of Antioxidant Metabolism and Cold-Responsive Genes in Three Bermudagrass Genotypes under Chilling and Freezing Stress.

As a typical warm-season grass, bermudagrass growth and turf quality begin to decrease when the environmental temperature drops below 20 °C. The current study investigated the differential responses of three bermudagrass genotypes to chilling stress (8/4 °C) for 15 days and then freezing stress (2/-2 °C) for 2 days. The three genotypes exhibited significant variation in chilling and freezing tolerance, and Chuannong-3, common bermudagrass 001, and Tifdwarf were ranked as cold-tolerant, -intermediate, and -sensitive genotypes based on evaluations of chlorophyll content, the photochemical efficiency of photosystem II, oxidative damage, and cell membrane stability, respectively.

Will the aging products of soil-reinforcement fibers stress plant growth and soil health?

Soil-reinforcement fibers are widely used for soil remediation and erosion prevention in ecologically vulnerable regions with sparse vegetation coverage and are incorporated into the soil for prolonged periods. However, the potential risks posed by aging fiber materials to soil health and plant growth have been largely neglected. This study explored the effects of aging solutions for polyethylene terephthalate (PET), coir, and carbon fibers on the physiological characteristics and vegetation coverage of ryegrass, as well as soil properties.

Genome-wide identification, expression analysis, and transcriptome analysis of the IAA gene family in Zoysia japonica.

Background: AUX/IAA is an essential signaling molecule and has great physiological importance in various plants, but its function in Zoysia japonica remains unknown.

Methods And Results: Genome-wide identification and analysis of AUX/IAA genes used bioinformatics methods to investigate the ZjIAA genes' expression of exogenous IAA hydroponics treatment for 2 h by qRT-PCR, control and exogenous IAA treated zoysia were subjected to transcriptome sequencing. ZjIAAs were distributed across the 13 subfamilies by phylogenetic analysis with Oryza sativa and Arabidopsis thaliana.

Overexpression of gene promotes leaf senescence in alfalfa participating in the hormone regulatory network.

Introduction: Alfalfa (Medicago sativa) is a kind of high quality leguminous forage species, which was widely cultivated in the world. Leaf senescence is an essential process in plant development and life cycle. Here, we reported the isolation and functional analysis of an alfalfa SENESCENCE-ASSOCIATED GENE113 (MsSAG113), which belongs to the PP2C family and mainly plays a role in promoting plant senescence.

Effects of exogenous organic/inorganic nitrogen addition on carbon pool distribution and transformation in grassland soil.

Aims: Increases in nitrogen (N) deposition may significantly affect the organic carbon (OC) cycle in soil. The inconsistent findings of the influence of added N on soil OC pools highlight the need of quantifying responses of the OC pool distribution to N addition. Moreover, the influence of N addition with a mixture of organic and inorganic N on OC pool distribution and stabilization in grassland soil remains unclear.

Metabolic Regulation and Lipidomic Remodeling in Relation to Spermidine-induced Stress Tolerance to High Temperature in Plants.

Beneficial effects of spermidine (Spd) on alleviating abiotic stress damage have been explored in plants for hundreds of years, but limited information is available about its roles in regulating lipids signaling and metabolism during heat stress. White clover () plants were pretreated with 70 μM Spd and then subjected to high temperature (38/33 °C) stress for 20 days. To further investigate the effect of Spd on heat tolerance, transgenic overexpressing a encoding a key enzyme involved in Spd biosynthesis was exposed to high temperature (38/33 °C) stress for 10 days.

Overexpression of from , which could interact with ABA2, improved plant cold tolerance mediated by ABA signaling.

ABI4 is considered an important transcription factor with multiple regulatory functions involved in many biological events. However, its role in abiotic stresses, especially low-temperature-induced stress, is poorly understood. In this study, the gene was derived from , a widely used forage grass.

Expression of a Hydroxycinnamoyl-CoA Shikimate/Quinate Hydroxycinnamoyl Transferase 4 Gene from () Causes Excessive Elongation and Lignin Composition Changes in .

Hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT) is considered to be an essential enzyme for regulating the biosynthesis and composition of lignin. To investigate the properties and function of , the gene was cloned from with a completed coding sequence of 1284-bp in length, encoding 428 amino acids. The gene promoter has several methyl jasmonate (MeJA) response elements.

Uncovering a Phenomenon of Active Hormone Transcriptional Regulation during Early Somatic Embryogenesis in .

Somatic embryogenesis (SE) is a developmental process in which somatic cells undergo dedifferentiation to become plant stem cells, and redifferentiation to become a whole embryo. SE is a prerequisite for molecular breeding and is an excellent platform to study cell development in the majority of plant species. However, the molecular mechanism involved in somatic embryonic induction, embryonic and maturation is unclear.

Progress and Challenges in China Turfgrass Abiotic Stress Resistance Research.

Turfgrasses are an important vehicle for urban ecology and one of the most important indicators of economy and civilization. The biological characteristics of different turfgrass species affect the productivity and quality of the turf and its potential use in landscapes, slopes, and sports fields. Cultivation and management techniques can assist turfgrasses to meet the challenges of climate change, while the development of molecular breeding will provide a broader platform for the application of turfgrasses.

Chlorophyll Reductase Gene Participates in Chlorophyll Degradation and Photosynthesis.

The degradation of chlorophyll is of great significance to plant growth. The chlorophyll reductase NOL (NYC1-like) is in charge of catalyzing the degradation of chlorophyll and maintaining the stability of the photosystem. However, the molecular mechanisms of NOL-mediated chlorophyll degradation, senescence, and photosynthesis and its functions in other metabolic pathways remain unclear, especially in warm-season turfgrass.

Turf Quality and Physiological Responses to Summer Stress in Four Creeping Bentgrass Cultivars in a Subtropical Zone.

Cool-season creeping bentgrass () has the ability to form fine sports turf, but high temperatures result in summer bentgrass decline (SBD), especially in transitional and subtropical zones. Physiological responses in combination with the alteration in turf quality (TQ) will contribute to a better understanding of SBD in a subtropical zone. Field experiments were conducted from 2017 to 2019 to test the adaptability to summer stress among four cultivars (13M, Penncross, Seaside II, and PA-1).

Polyamines Metabolism Interacts with γ-Aminobutyric Acid, Proline and Nitrogen Metabolisms to Affect Drought Tolerance of Creeping Bentgrass.

Due to increased global warming and climate change, drought has become a serious threat to horticultural crop cultivation and management. The purpose of this study was to investigate the effect of spermine (Spm) pretreatment on metabolic alterations of polyamine (PAs), γ-aminobutyric acid (GABA), proline (Pro), and nitrogen associated with drought tolerance in creeping bentgrass (). The results showed that drought tolerance of creeping bentgrass could be significantly improved by the Spm pretreatment, as demonstrated by the maintenance of less chlorophyll loss and higher photosynthesis, gas exchange, water use efficiency, and cell membrane stability.

Transcriptome profiling revealed candidate genes, pathways and transcription factors related to nitrogen utilization and excessive nitrogen stress in perennial ryegrass.

Ryegrass (Lolium perenne L.), a high-quality forage grass, is a good nutrient source for herbivorous livestock. However, improving nitrogen use efficiency and avoiding nitrate toxicity caused by excessive nitrogen are continual challenges in ryegrass production.

Transcriptome Analysis Revealed a Positive Role of Ethephon on Chlorophyll Metabolism of under Cold Stress.

is a warm-season turfgrass with a good tolerance and minimal maintenance requirements. However, its use in Northern China is limited due to massive chlorophyll loss in early fall, which is the main factor affecting its distribution and utilization. Although ethephon treatment at specific concentrations has reportedly improved stress tolerance and extended the green period in turfgrass, the potential mechanisms underlying this effect are not clear.

Expression of , a Phytoene Synthase Gene from Affects Plant Height and Photosynthetic Pigment Contents.

Phytoene synthase (PSY) is a key limiting enzyme in the carotenoid biosynthesis pathway for regulating phytoene synthesis. In this study, was isolated and identified from , an important lawn grass species. cDNA was 1230 bp in length, corresponding to 409 amino acids.

Effects of Elevation and Distance from Highway on the Abundance and Community Structure of Bacteria in Soil along Qinghai-Tibet Highway.

In recent years, highway construction in the Qinghai-Tibet Plateau (QTP) has developed rapidly. When the highway passes through grassland, the soil, vegetation, and ecological environment along the line are disturbed. However, the impact on soil bacteria is still unclear.

Transcriptome analysis of Kentucky bluegrass subject to drought and ethephon treatment.

Kentucky bluegrass (Poa pratensis L.) is an excellent cool-season turfgrass utilized widely in Northern China. However, turf quality of Kentucky bluegrass declines significantly due to drought.

Adaptability to High Temperature and Stay-Green Genotypes Associated With Variations in Antioxidant, Chlorophyll Metabolism, and γ-Aminobutyric Acid Accumulation in Creeping Bentgrass Species.

High temperature limits the cultivation and utilization of cool-season plants in many regions worldwide. Recently, extreme hot waves swept across the globe in summer, leading to enormous economic loss. The evaluation and identification of genotypic variation in thermotolerance within species are critical to breeding for environmental adaptation and also provide potential materials to explore thermo-resistant mechanism in plants.

Melatonin influences the early growth stage in Zoysia japonica Steud. by regulating plant oxidation and genes of hormones.

Zoysia japonica is a commonly used turfgrass species around the world. Seed germination is a crucial stage in the plant life cycle and is particularly important for turf establishment and management. Experiments have confirmed that melatonin can be a potential regulator signal in seeds.

Transcriptional regulation and stress-defensive key genes induced by γ-aminobutyric acid in association with tolerance to water stress in creeping bentgrass.

γ-Aminobutyric acid (GABA) acts as an important regulator involved in the mediation of cell signal transduction and stress tolerance in plants. However, the function of GABA in transcriptional regulation is not fully understood in plants under water stress. The creeping bentgrass () was pretreated with or without GABA (0.

Chlorophyll Metabolism and Gene Expression in Response to Submergence Stress and Subsequent Recovery in Perennial Ryegrass Accessions Differing in Growth Habits.

Submergence-induced leaf senescence may alter chlorophyll metabolism. The objective of this study was to characterize chlorophyll biosynthesis and degradation in contrasting perennial ryegrass (Lolium perenne) in response to submergence stress and recovery. The light-green and fast-growing accession PI238938 and the darker-green and slow-growing cultivar BrightStar SLT were exposed to 0, 6 h, 1-, 3-, and 7-d of submergence stress and 1- and 5- d of de-submergence, respectively.