Publications by authors named "Han Hsiao"
Recent advances in high-throughput genomic technologies have nurtured a growing demand for statistical tools to facilitate identification of molecular changes as potential prognostic biomarkers or drugable targets for personalized precision medicine. In this study, we developed a web-based interactive and user-friendly platform for high-dimensional analysis of molecular alterations in cancer (HDMAC) (https://ripsung26.shinyapps.
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- Plant protoplasts are effective for evaluating CRISPR/Cas9 mutagenesis efficiency, and the authors enhanced methods for isolating and transfecting these protoplasts from various plant species.
- They created a technique to isolate and regenerate transformed Nicotiana tabacum protoplasts into mature plants, allowing for the analysis of mutagenesis success.
- In their study, all four alleles of the phytoene desaturase (NtPDS) gene were mutated in regenerated plants, and most did not contain detectable Cas9 DNA.
The chloroplast NAD(P)H dehydrogenase-like (NDH) complex consists of about 30 subunits from both the nuclear and chloroplast genomes and is ubiquitous across most land plants. In some orchids, such as Phalaenopsis equestris, Dendrobium officinale and Dendrobium catenatum, most of the 11 chloroplast genome-encoded ndh genes (cp-ndh) have been lost. Here we investigated whether functional cp-ndh genes have been completely lost in these orchids or whether they have been transferred and retained in the nuclear genome.
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