Statistical Experimental Design as a New Approach to Optimize a Solid-State Fermentation Substrate for the Production of Spores and Bioactive Compounds from .

Managing organic agricultural wastes is a challenge in today's modern agriculture, where the production of different agricultural goods leads to the generation of large amounts of waste, for example, olive pomace and vine shoot in Mediterranean Europe. The discovery of a cost-effective and environment-friendly way to valorize such types of waste in Mediterranean Europe is encouraged by the European Union regulation. As an opportunity, organic agricultural waste could be used as culture media for solid-state fermentation (SSF) for fungal strains.

Using bibliometrics to analyze the state of art of pesticide use in vineyard agrosystems: a review.

The production of wine dates to ancient civilization and has heavily influenced different landscapes around the globe. Nowadays, wine is still an important sector in terms of land use and income source in many countries, more specifically in the European Mediterranean region. However, to control pests and regulate wine growth, the extensive use of pesticides is common.

Biotechnological potential of Zymotis-2 bioreactor for the cultivation of filamentous fungi.

Background/aim: A new prototype of Solid-State Fermentation Bioreactor, namely "Zymotis-2 ", was developed to produce fungal spores.

Main Methods And Major Results: A fermentation process for fungal spores, and hydrolase enzymes (endo and exoglucanases, amylases) production by Trichoderma asperellum DWG3, Aspergillus niger G131 and Beauveria bassiana was scaled-up from flasks and glass Raimbault column packed with 20 g of solid substrates (dry weight) to 5 kg of solid substrate by using the new Zymotis-2 bioreactor. Fungi strains growth using a mix of vine shoots, wheat bran, and olive pomace was tested under similar experimental conditions in Zymotis-2 bioreactor, column bioreactor and flasks in a parallel fermentation system.

Statistical approach to evaluate effect of temperature and moisture content on the production of antioxidant naphtho-gamma-pyrones and hydroxycinnamic acids by Aspergillus tubingensis in solid-state fermentation.

Non-ochratoxigenic Aspergillus tubingensis G131 is a filamentous fungus that can produce naphtho-gamma-pyrones (NγPs), polyketide pigments that exhibit interesting antioxidant properties. This study aims to investigate the effect of two critical parameters, temperature and moisture content on the fungus grown in solid-state fermentation using agricultural by-products (vine shoots and wheat bran) as sole medium. From the kinetic productions of secondary metabolites NγPs (asperpyrone E, dianhydroaurasperone C, fonsecin, fonsecin B and ustilaginoidin A), alkaloids (nigragilin and aspernigrin A), degradation products from the solid medium (β-D-glucose, p-coumaric acid and trans-ethyl ferulate), ergosterol and conidia obtained for different temperatures and moisture contents, a principal component analysis (PCA) was carried out to highlight the production patterns of these compounds.

Naphtho-Gamma-Pyrones Produced by G131: New Source of Natural Nontoxic Antioxidants.

Seven naphtho-gamma-pyrones (NγPs), including asperpyrone E, aurasperone A, dianhydroaurasperone C, fonsecin, fonsecinone A, fonsecin B, and ustilaginoidin A, were isolated from G131, a non-toxigenic strain. The radical scavenging activity of these NγPs was evaluated using ABTS assay. The Trolox equivalent antioxidant capacity on the seven isolated NγPs ranged from 2.

From flasks to single used bioreactor: Scale-up of solid state fermentation process for metabolites and conidia production by Trichoderma asperellum.

Currently, the increasing demand of biopesticides production to replace chemical pesticides which are excessively used has made solid state fermentation (SSF) technology the need of the hour. In spite of advantages, true potential of SSF process has not been fully realized at industrial scale. A fermentation process for 6-pentyl-a-pyrone (6 PP), conidia, and lytic enzymes (cellulases, lipase, amylase) production by Trichoderma asperellum TF1 was scaled-up from 250 mL flasks and glass Raimbault column packed with 20 g of solid substrates (dry weight) to 5 Kg of solid substrate by using a new plastic single used bioreactor.