Adenine nucleotide content in cold preserved human donor hearts and subsequent cardiac performance after orthotopic heart transplantation.

Myocardial high-energy phosphate content has been used as a parameter to evaluate the adequacy of donor organ preservation. The purpose of this study was to assess current techniques of preservation by measuring high-energy phosphates in cold preserved (4 degrees C) human donor hearts. Endomyocardial biopsy samples of the donor heart right ventricular septum (n = 24) were compared with samples from patients with normal cardiac function evaluated before chemotherapy (n = 12).

Simulation of cell rolling and adhesion on surfaces in shear flow. Microvilli-coated hard spheres with adhesive springs.

The adhesion of cells to ligand-coated surfaces in viscous shear flow is an important step in many physiological processes, such as the neutrophil-mediated inflammatory response, lymphocyte homing, and tumor cell metastasis. This article describes a calculational method that allows simulation of the interaction of a single cell with a ligand-coated surface. The cell is idealized as a microvilli-coated hard sphere covered with adhesive springs.

Adaptive behavior of preschool children with developmental delays: parent versus teacher ratings.

Parents and teachers of 20 children with developmental delays, 24 through 38 months of age, from an early education program in Columbus, Ohio, were interviewed using the Adaptive Behavior Scale for Infants and Early Childhood. Significant differences were found between some of the ratings over the 23 domain and 11 subdomain scores, specifically in regard to sex of the child and length of time of the child's participation in the program. Parents provided higher ratings than did teachers on some domains.

General analysis of receptor-mediated viral attachment to cell surfaces.

Viruses are multivalent particles that attach to cells through one or more bonds between viral attachment proteins (VAP) and specific cellular receptors. Three modes of virus binding are presented that can explain the diversity in binding data observed among viruses. They are based on multivalency of attachment and spatial versus receptor saturation effects which are easily distinguished based upon simple criteria.

Organ-preference of metastasis. The role of endothelial cell adhesion molecules.

The initial, site-specific colonization of secondary organs by blood-borne cancer cells appears to be mediated by endothelial cell adhesion molecules. These molecules are part of the organ-specific microvascular phenotype and are regulated through complex interactions of the endothelium with the extracellular matrix (e.g.

Nucleotide profiles in normal minipig arterial tissue.

The purpose of this study was to characterize the nucleotide profiles of a normal porcine elastic and muscular artery. Tissue samples (50 to 150 mg) were excised from the descending thoracic aorta and from the femoral artery of 14 normocholesterolemic, anesthetized minipigs. In three animals, transmural myocardial samples were also obtained.

Relationship between enterotoxic- and T lymphocyte-stimulating activity of staphylococcal enterotoxin B.

The enterotoxins produced by Staphylococcus aureus cause a gastrointestinal intoxication probably via their action on intramucosal neuronal cells. Staphylococcal enterotoxins are also the most powerful mitogens known, activating CD3+ T lymphocytes of several species in a clonally variable and MHC class II-dependent fashion. We examined a possible relationship between enterotoxic and mitogenic activity of staphylococcal enterotoxin serotype B (SEB).

Bioreactor development for production of viral pesticides or heterologous proteins in insect cell cultures.

The insect cell-baculovirus expression system has significant potential for producing proteins requiring some degree of posttranslational modification. T. ni cells appear to be as good a host as S.

[Uncommon surgical treatment of a tibial fibrous bone dysplasia].

The authors present an original method of treatment in prevention of fracture of the tibial shaft applied in one case of polyostosic fibrous dysplasia of bone. They treated this localization by closed intramedullary reaming and stuffage with cancellous bone grafts.

Role of substance P in immediate-type skin reactions induced by staphylococcal enterotoxin B in unsensitized monkeys.

The staphylococcal enterotoxin B (SEB)-induced immediate-type skin reaction in unsensitized monkeys was used as a nonimmunologic mast cell stimulation to search for possible involvement of local neural mechanisms. Evidence is presented that substance P (SP) plays a predominant role in mediating intradermal SEB challenge in unsensitized monkeys. With a rabbit SP antiserum directed against the C-terminal region of SP, a concentration-dependent inhibition of SEB-induced skin reactivity could be demonstrated.

New focal plane architecture and transform for fast target recognition.

This paper presents a new concept for recognizing target shapes. The method depends upon establishing a uniform current density in a mesh of interconnected photosensitive pixels and characterizing the target image by potential changes at the mesh boundary. The concept proposes a way to reduce potentially hundreds of thousands of pixel values to a small set of signature values.

A dynamical model for receptor-mediated cell adhesion to surfaces in viscous shear flow.

We present a dynamical model for receptor-mediated cell adhesion to surfaces in viscous shear flow when the surfaces are coated with ligand molecules complementary to receptors in the cell membrane. This model considers the contact area between the cell and the surface to be a small, homogeneous region that mediates the initial attachment of the cell to the surface. Using a phase plane analysis for a system of nonlinear ordinary differential equations that govern the changes in free receptor density and bond density within the contact area with time, we can predict the conditions for which adhesion between the cell and the surface will take place.

Hot refueling hazards.

Hot refueling in the special operations community has several potential hazards which can accompany the operation. The MC-130 Panel Operator's (PO) position is located in the exhaust of the number 3 engine, subjecting him to fumes, heat, and exhaust blast. The hypothesis was that time constraints should be implemented to the work load due to heat and carbon monoxide exposure.

The reaction-limited kinetics of membrane-to-surface adhesion and detachment.

Biological adhesion is frequently mediated by specific membrane proteins (adhesion molecules). Starting with the notion of adhesion molecules, we present a simple model of the physics of membrane-to-surface attachment and detachment. This model consists of coupling the equations for deformation of an elastic membrane with equations for the chemical kinetics of the adhesion molecules.

Protection against the staphylococcal enterotoxin-induced intestinal disorder in the monkey by anti-idiotypic antibodies.

The staphylococcal enterotoxin serotype B (SEB)-induced enteric intoxication and the immediate-type reaction in the skin of unsensitized monkeys was used to define whether agents competing with SEB for target cell receptors may inhibit pathophysiological effects. For this purpose a duodenal provocation test was developed by use of a pediatric gastroscope, allowing the evaluation of the influence of antagonists on the intestinal disorder upon SEB challenge at the same duodenal site. First, carboxymethylation of histidine residues of SEB caused a complete loss of emetic and skin-sensitizing activity without changing the immunological specificity.

Extraction and measurement of myocardial nucleotides, nucleosides, and purine bases by high-performance liquid chromatography.

Nucleotides, nucleosides, and purine bases were extracted from human endomyocardial biopsies, freeze-clamped rat hearts, and porcine coronary sinus plasma. Perchloric acid extracts were neutralized with Freon-trioctylamine and analyzed at 250 nm by reverse-phase ion-pairing high-performance liquid chromatography. To achieve the sensitivity necessary for analyzing small (1-3 mg wet wt) tissue samples, a small-bore, 2.

Cysteinyl leukotrienes as mediators of staphylococcal enterotoxin B in the monkey.

The role of cysteinyl leukotrienes (LTs) in the action of staphylococcal enterotoxin B (SEB) was investigated in unsensitized monkeys using inhibitors of prostanoid synthesis and LT action and by measuring generation of LT in vivo. LY 171883, a selective LTD4/LTE4 receptor antagonist, proved highly efficient in inhibiting immediate-type hypersensitivity reactions in the skin and protecting against the emetic response provoked by SEB in a concentration-dependent manner. Inhibition of prostanoid formation by pretreatment of monkeys with indomethacin or aspirin did not influence SEB responses.

A dynamical model for receptor-mediated cell adhesion to surfaces.

We present a dynamical model for receptor-mediated adhesion of cells in a shear field of viscous fluid to surfaces coated with ligand molecules complementary to receptors in the cell membrane. We refer to this model as the "point attachment model" because it considers the contact area between the cell and the surface to be a small, homogeneous region that mediates the initial attachment of the cell to the surface. Using a phase plane analysis of a system of nonlinear ordinary differential equations which govern the changes in free receptor density and bond density within the contact area with time, we can predict the conditions for which adhesion between the cell and the surface will take place.

[Ender's so-called alignment nailing in the treatment of trochanteric fractures in the young adult, and under certain circumstances: is it reasonable? Study of a series of 50 cases].

The technique and results of an original method of "alignment Ender's nailing", combining standard Ender's nailing with post-operative traction is described. This method is directed towards young patients in two well-defined situations: the complexity of the trochanteric fracture and the debilitation of the patient. A consecutive series of 50 cases have been analysed.