Spontaneous chemiluminescence activity of peripheral blood cells during experimental allergic neuritis (EAN).

In 30 Lewis rats the time course of spontaneous monocyte and granulocyte chemiluminescence activity (CLA) during myelin-induced experimental allergic neuritis (EAN) was studied. CLA of blood and peritoneal cells, and clinical, histopathological and electrophysiological findings were compared with data from 14 animals immunized with complete Freund's adjuvant and ten normal Lewis rats. About 12 days post-immunization there was a significant (p less than 0.

The significance of the inflammatory reactions for the development of clinical signs in multiple sclerosis and acute experimental autoimmune encephalomyelitis as assessed by means of the spontaneous chemiluminescence activity of peripheral blood monocytes.

We found that the course of the chemiluminescence activity (CL-A) of peripheral blood monocytes of Lewis rats with acute experimental autoimmune encephalomyelitis (EAE) correlates well with the clinical course of this autoimmune disease and that only a reduction in T helper cells caused a reduction in CL-A. However, in multiple sclerosis (MS) patients the CL-A increased when clinical improvement started. The serum of these patients contained at least two stimulatory substances.

The inhibitory effects of prednisone, 16-methylen-prednisolone, and ACTH on con-A induced lymphokines (interferon-y) as measured by the chemiluminescence-activity of blood monocytes.

When lymphocytes are stimulated with mitogens or antigens they are enhanced via a cascade of lymphokines to produce interferon-y (IFN-y). IFN-y augments the H2O2 secretion of human monocytes which indirectly can be measured by chemiluminescence. We tested prednisone, 16-methylen-prednisolone and ACTH for their effect to inhibit the Con-A induced stimulation of the chemiluminescence-activity.

Acute adrenocortical stimulation by recombinant gamma interferon in human controls.

We examined the effect of 20 micrograms recombinant gamma-interferon (rec-gamma-IFN) upon corticotropin (ACTH) and cortisol secretion in 10 healthy male controls. We observed that rec-gamma-IFN enhances cortisol secretion with maxima around 3 hours after injection of the test dose. This effect was suppressible by a single dose of 1.

The chemiluminescence activity of peripheral blood mononuclear cells during acute experimental allergic encephalomyelitis.

The spontaneous chemiluminescence activity (CL-A) of peripheral mononuclear cells (MNC) was examined in Lewis rats with acute experimental allergic encephalomyelitis (EAE), compared to rats immunized with complete adjuvant (n = 11) and healthy animals (n = 16). In rats with EAE, CL-A increased sharply 8-9 days after immunization (3420 +/- 3124 counts/10 s, n = 16) at the time of flattening of the weight curve. This CL-A peak was compared to that of animals immunized with complete adjuvant: 765 +/- 441 counts/10 s (P = 0.

The behaviour of OKT3-, OKT4- and OKT8-positive cells during phases of elevated spontaneous chemiluminescence activity (CL-A) in multiple sclerosis patients. A serial study.

The chemiluminescence activity (CL-A; synonym = burst activity, BA) and the percentage of OKT3-, OKT4- and OKT8-positive peripheral blood cells were serially examined in four control persons and in eight patients with multiple sclerosis. When the OKT values obtained in phases of increased CL-A (clinical remission) were compared with those of the control group, the percentage of OKT3-positive cells was reduced (P = 0.014), and that of OKT4-positive cells increased (P = 0.

Determination of interferon-gamma in the peripheral blood of multiple sclerosis patients in remission, using a chemiluminescence technique.

The plasma from eight patients with multiple sclerosis (MS) whose disease was in remission, was investigated by a chemiluminescence technique for its ability to stimulate the oxidative metabolism of peripheral blood monocytes (PBM). The active fraction identified had a molecular weight between 13,700 and 43,000 Da. Its activity was reduced by incubation at pH 2, pH 4 or pH 6, or by treatment at 56 degrees C for 1-3 h.

The spontaneous burst activity of peripheral blood monocytes in patients with acute polyradiculoneuritis, lymphocytic meningoencephalitis, and multiple sclerosis. A comparison.

The course of the spontaneous burst activity (BA) of peripheral blood monocytes was examined in patients with acute polyradiculoneuritis (PN), lymphocytic meningoencephalitis (LE), and multiple sclerosis (MS) and the BA was compared with the clinical course. In 4 patients with postinfectious acute PN the BA was significantly increased up to values around 60 000 counts/10 s. The BA and the clinical course were closely correlated in these patients (mean of r = 0.

Monocytes constitute the only peripheral blood cell population showing an increased burst activity in multiple sclerosis patients.

We examined peripheral mononuclear cells (PMNC), peripheral polymorphonuclear cells (PPNC), adherent mononuclear cells, non-adherent mononuclear cells, non-phagocytic mononuclear cells and non-phagocytic Percoll-fractionated mononuclear cells of multiple sclerosis (MS) patients for their spontaneous burst activity (BA). According to previous results PMNC of MS patients showed a markedly increased BA (p = 0.0002); PPNC, however, did not show significantly elevated values (p = 0.

Indications of the occurrence of inflammatory reactions in the clinical improvement phase in multiple sclerosis patients.

In patients with multiple sclerosis (MS) the spontaneous burst activity (BA) of peripheral blood mononuclear cells was related to the clinical course of the disease. In five patients clinical improvement was found while the BA was significantly increased (more than 300% of the controls). During the appearance of new or deteriorating signs and in the period without clinical changes, the BA was not at all or not markedly increased.

Correlation of the chemiluminescence-activity of peripheral blood monocytes with CSF parameters of inflammation and the clinical course of patients with lymphocytic meningoencephalitis.

The chemiluminescence-activity (CL-A) of peripheral blood monocytes (MO) was measured in eight patients with lymphocytic meningitis or meningoencephalitis and compared to CSF parameters and the clinical course. The initial maximum CL-A was around four times above the control and decreased to normal values within approximately 20 days. Poor correlations were found when the CL-A was compared to CSF parameters in the total group of patients.

Hemichorea associated with varicella-zoster reinfection and endocarditis. A case report.

A 20-year-old woman developed transient right-sided hemichoreatic movements after household exposure to varicella-zoster. Some days before the appearance of involuntary movements a vesicular rash had occurred. About 6 months later an elevated IgG serum titer against varicella virus was found and two-dimensional echocardiography showed signs of an endocarditis.

Changes in the number of complement-binding leucocytes in the peripheral blood of multiple sclerosis (MS) patients: indication to a reduction of EAC3bi-binding T cells in the early acute phase of MS.

Peripheral blood leucocytes (PBL) of multiple sclerosis (MS) patients and healthy controls were examined for their reactivity with EAC3b, EAC3b-beta 1H, and EAC3bi intermediates and C3b- or beta 1H-coated microspheres (ms). A marked decrease was found in the number of EAC3b-beta 1H (P = 0.04) and EAC3bi (P = 0.

Measurement of the respiratory burst in human monocytes and polymorphonuclear leukocytes by nitro blue tetrazolium reduction and chemiluminescence.

We compared measurements of chemiluminescence (CL) assessing the rate of production of oxygen intermediates at a given instance, and of nitro blue tetrazolium (NBT) reduction detecting total superoxide produced during the assay period, for the assessment of the respiratory burst of both human monocytes (M phi) and polymorphonuclear leukocytes (PMN). In the CL experiments, opsonized and non-opsonized zymosan was used to stimulate peripheral blood M phi and PMN. Opsonized zymosan yielded an earlier and 2-fold higher peak response than non-opsonized zymosan.

Multiple sclerosis patients show an increased spontaneous activity of their peripheral blood monocytes as measured by chemiluminescence.

I has been reported that myelin basic protein (BP) reacts extremely sensitively to peroxide, which is formed when monocytes/macrophages are stimulated to produce a "respiratory burst" (RB). We measured the RB activity by means of chemiluminescence in peripheral blood monocytes/macrophages (MO) of 17 MS patients, 5 patients with a viral infection of the CNS, and 14 control persons. The median of the spontaneous RB activity of MS patients compared with the median of our control group showed a highly significant increase (P = 0.

Binding of the third component of complement (C3) to Thy-1.2 positive Con-A-induced blasts of the murine spleen: modulation of the C3 binding and cell aggregation by protease inhibitors.

The binding of the third component of the complement system (C3) to Con-A-stimulated murine spleen cells (MSPC) and the role of C3 for the formation of cell aggregates was examined. C3 became bound up to 15% of the T blasts (Thy-1.2 positive cells) while only 2.

Conditions for the enhancing effect of protease inhibitors on the concanavalin A induced thymidine response of murine lymphocytes.

Incorporation of [3H]-thymidine - [3H]-TdR - into concanavalin A (Con A) stimulated murine splenocytes and thymocytes was found to be enhanced by addition of certain concentrations of phenyl-methylsulfonylfluoride (PMSF), di-isopropylfluorophosphate (DFP), N-alpha-tosyl-L-lysyl-L-chloromethylketone (TLCK), and soybean trypsin inhibitor (SBTI). No enhancement could be observed when mononuclear cells of the peripheral blood were used, and a medium enhancement when thymocytes were applied. Furthermore, no enhancing effect of the protease inhibitors (PI) on the Con A response of murine splenocytes could be observed within the first 24 h of the culturing period.

Differential effect of low molecular weight alcohols on the Con A stimulation of mouse spleen cells.

Incorporation of [3h]thymidine ([3H]TdR) into concanavalin A (Con A)-stimulated murine splenocytes was found to be enhanced by addition of certain concentrations of ethanol, 2-propanol and acetone. The alcohol/acetone-induced enhancement of the Con A response was found to be accompanied by an increase of the percentage of living cells as assessed by trypan blue exclusion. Concentrations of ethanol and 2-propanol which caused maximum [3H]TdR uptake in Con A cultures were also found to lead to higher percentages of aggregated cells than in comparison to Con A cultures without alcohol.

Activation of human monocytes by both human beta 1H and C3b.

We tested whether highly purified human β1H and C3b, two proteins of the alternative pathway of complement activation, could exert an influence on the activity of human monocytes (Mφ). The activation process of Mφ was assessed by measurements of the respiratory burst in terms of nitro blue tetrazolium (NBT) reduction and by chemiluminescence (CL) tests. In NBT reduction experiments, we found a tendency for β1H to increase NBT reduction, while C3b was found to be rather inhibitory.

A comparative evaluation of receptor reactivities for C3b, iC3b, and C3d on Raji lymphoblastoid cells.

Raji cells were described to carry receptors for iC3b, C3d, C3b-beta 1 H and beta 1 H. Controversial opinions, however, exist whether or not these cells carry also receptors for C3b. Using highly purified C3, definitely devoid of beta 1 H and C5, for preparation of C3b intermediates, it could be shown that Raji cells bound to C3b cells.

Role of beta 1H for the binding of C3b-coated particles to human lymphoid and phagocytic cells.

Coating of EAC14oxy23b with highly purified human serum beta 1H globulin (beta 1H) led to acceleration of rosette formation with human peripheral blood lymphocytes (PBL), tonsil lymphocytes, B lymphoblastoid (Raji) cells, granulocytes and monocytes. This reaction was discernible from C3bi-dependent rosette formation. Enhancement of rosette formation of C3b cells by beta 1H was most effective at limiting amounts of C3 per EAC14oxy23b.