Sensitivities of a bulk-tank milk ELISA and composite fecal qPCR to detect various seroprevalence levels of paratuberculosis in cattle herds in Normandy, France.

This study evaluated an ELISA on bulk tank milk (BTM) samples and a qPCR on a single composite fecal sample to detect paratuberculosis seropositive cattle dairy herds. Individual serum ( = 15 372), BTM and composite fecal samples were obtained from 192 herds. The within-herd apparent seroprevalence was categorized and compared with BTM ELISA and fecal qPCR results.

Trueness and precision of the real-time RT-PCR method for quantifying the chronic bee paralysis virus genome in bee homogenates evaluated by a comparative inter-laboratory study.

The Chronic bee paralysis virus (CBPV) is the aetiological agent of chronic bee paralysis, a contagious disease associated with nervous disorders in adult honeybees leading to massive mortalities in front of the hives. Some of the clinical signs frequently reported, such as trembling, may be confused with intoxication syndromes. Therefore, laboratory diagnosis using real-time PCR to quantify CBPV loads is used to confirm disease.