A high copy suppressor screen identifies factors enhancing the allotopic production of subunit II of cytochrome c oxidase.

Allotopic expression refers to the artificial relocation of an organellar gene to the nucleus. Subunit 2 (Cox2) of cytochrome c oxidase, a subunit with two transmembrane domains (TMS1 and TMS2) residing in the inner mitochondrial membrane with a Nout-Cout topology, is typically encoded in the mitochondrial cox2 gene. In the yeast Saccharomyces cerevisiae, the cox2 gene can be allotopically expressed in the nucleus, yielding a functional protein that restores respiratory growth to a Δcox2 null mutant.

Nature-based solutions for improving food security: A systematic global review.

Nature-based solutions (NBS) have been promoted as a holistic way to solve a variety of societal issues while benefiting biodiversity at the same time. To date, applications of NBS approaches that help ensure food security have yet been systematically reviewed. In this paper, we critically review the specific NBS for food security, highlighting their limitations, to provide recommendations that promote their applications for improving global food security.

Vitamin B5 metabolism is essential for vacuolar and mitochondrial functions and drug detoxification in fungi.

Fungal infections, a leading cause of mortality among eukaryotic pathogens, pose a growing global health threat due to the rise of drug-resistant strains. New therapeutic strategies are urgently needed to combat this challenge. The PCA pathway for biosynthesis of Co-enzyme A (CoA) and Acetyl-CoA (AcCoA) from vitamin B5 (pantothenic acid) has been validated as an excellent target for the development of new antimicrobials against fungi and protozoa.

Identification of factors limiting the allotopic production of the Cox2 subunit of yeast cytochrome c oxidase.

Mitochondrial genes can be artificially relocalized in the nuclear genome in a process known as allotopic expression, such is the case of the mitochondrial cox2 gene, encoding subunit II of cytochrome c oxidase (CcO). In yeast, cox2 can be allotopically expressed and is able to restore respiratory growth of a cox2-null mutant if the Cox2 subunit carries the W56R substitution within the first transmembrane stretch. However, the COX2W56R strain exhibits reduced growth rates and lower steady-state CcO levels when compared to wild-type yeast.

Nonsynonymous Mutations in Intellectual Disability and Autism Spectrum Disorder Gene PTCHD1 Disrupt -Glycosylation and Reduce Protein Stability.

has been implicated in Autism Spectrum Disorders (ASDs) and/or intellectual disability, where copy-number-variant losses or loss-of-function coding mutations segregate with disease in an X-linked recessive fashion. Missense variants of have also been reported in patients. However, the significance of these mutations remains undetermined since the activities, subcellular localization, and regulation of the PTCHD1 protein are currently unknown.

Dataset of urban nature games to aid integrating nature-based solutions in urban planning.

This dataset of Urban Nature Games provides information, ratings, and categorizations of different types of games that incorporate concepts of urban planning and ecosystem services or nature-based solutions. It consists of games retrieved from systematic searches on various search engines and public databases, using keywords related to: urban design and planning; ecosystem services and nature-based solutions; and game-based approaches. Recorded meta information includes game names, developers, links to each game's documentation, relevant publications, as well as generic playing information such as play duration, number of players, target group, distribution format, play mode, and costs.

Neuronal transcription of autism gene PTCHD1 is regulated by a conserved downstream enhancer sequence.

Patched domain-containing 1 (PTCHD1) is a well-established susceptibility gene for autism spectrum disorder (ASD) and intellectual disability (ID). Previous studies have suggested that alterations in the dosage of PTCHD1 may contribute to the etiology of both ASD and ID. However, there has not yet been a thorough investigation regarding mechanisms that regulate PTCHD1 expression.

Computed Tomographic Evaluation of Safe Corridors for Pin Placement in Rabbit Thoracolumbar Vertebrae and Sacrum.

Objective:  The aim of this study was to identify safe corridors for pin placement in the terminal thoracic vertebrae, lumbar vertebrae and sacrum of rabbits using computed tomography (CT) in cadaveric models.

Study Design:  Computed tomographic imaging of 25 adult New Zealand white rabbit () cadavers was evaluated. Safe insertion corridors at the cranial and caudal end cortical sections (ECS) of the vertebrae were determined using a multiplanar reconstruction software.

Management of vernal keratoconjunctivitis: Navigating a changing treatment landscape.

Vernal keratoconjunctivitis (VKC) is a chronic, progressive, and potentially sight-threatening form of ocular inflammatory disease that primarily affects children and young adults. Prevalence varies by region, ranging from <2 per 10,000 in the United States to as high as 1,100 per 10,000 in parts of Africa. The rarity of VKC in developed countries can make differential diagnosis challenging, and treatment is often delayed until the disease is advanced, and symptoms are significantly impacting patients' quality of life.

Ex vivo comparison of pin placement with patient-specific drill guides or freehand technique in canine cadaveric spines.

Objective: To compare vertebral implant placement in the canine thoracolumbar spine between 3D-printed patient-specific drill guides (3DPG) and the conventional freehand technique (FH).

Study Design: Ex vivo study.

Animals: Cadaveric canine spines (n = 24).

The constraints of allotopic expression.

Allotopic expression is the functional transfer of an organellar gene to the nucleus, followed by synthesis of the gene product in the cytosol and import into the appropriate organellar sub compartment. Here, we focus on mitochondrial genes encoding OXPHOS subunits that were naturally transferred to the nucleus, and critically review experimental evidence that claim their allotopic expression. We emphasize aspects that may have been overlooked before, i.

Two disulfide-reducing pathways are required for the maturation of plastid c-type cytochromes in Chlamydomonas reinhardtii.

In plastids, conversion of light energy into ATP relies on cytochrome f, a key electron carrier with a heme covalently attached to a CXXCH motif. Covalent heme attachment requires reduction of the disulfide-bonded CXXCH by CCS5 and CCS4. CCS5 receives electrons from the oxidoreductase CCDA, while CCS4 is a protein of unknown function.

Leaf acclimation to soil flooding and light availability underlies photosynthetic capacity of , an endangered shrub of bottomland forests in the Mississippi Alluvial Valley, USA.

is an endangered shrub indigenous to the broadleaf forest of the Mississippi Alluvial Valley (MAV). In this region, extant colonies of the species are found in periodically ponded habitats where a diversity of broadleaf trees can form well-developed overstory and sub-canopies-these habitat characteristics suggest that soil flooding and light availability are primary drivers of ecophysiology. To understand how these two factors affect its photosynthetic capacity, we quantified leaf characteristics and photosynthetic response of plants grown in a large-scaled, field setting of three distinct soil flooding levels (no flood, 0 day; short-term flood, 45 days; and extended flood, 90 days) each containing three distinct light availability levels (high light, 30% shade cloth; intermediate light, 63% shade cloth; and low light, 95% shade cloth).

Ala vestibuloplasty improves cardiopulmonary and activity-related parameters in brachycephalic cats.

Objective: To evaluate the effects of ala vestibuloplasty on cardiopulmonary and lifestyle-related parameters in brachycephalic (BC) cats.

Study Design: Prospective cohort.

Animals: Client-owned BC cats (n = 19).

An Infant with Bilateral Keratitis: From Infectious to Genetic Diagnosis.

BACKGROUND Tyrosinemia Type II (TYRII) is a rare autosomal recessive inborn error of metabolism caused by deficiency of tyrosine aminotransferase (TAT), leading to hypertyrosinemia. TYRII patients often present in the first year of life with ocular and cutaneous findings, including corneal ulcers, pseudodendritic keratitis, and palmoplantar hyperkeratosis. The corneal involvement is often mistaken for herpes simplex virus (HSV) keratitis, which is a much commoner condition.

Disseminated juvenile xanthogranulomas with ocular involvement: A case report and literature review.

Cutaneous juvenile xanthogranuloma is an uncommon disorder usually arising during infancy. Systemic involvement of juvenile xanthogranuloma remains rare, and there are no published guidelines to date on screening extracutaneous manifestations in these patients. Ocular involvement is the most common extracutaneous manifestation of juvenile xanthogranuloma.

Long-term outcomes of type 1 retinopathy of prematurity following monotherapy with bevacizumab: a Canadian experience.

Objective: To report long-term structural, visual, and refractive outcomes after monotherapy with intravitreal bevacizumab injection.

Design: Cohort retrospective chart review.

Participants: A total of 56 premature infants with type 1 retinopathy of prematurity.

: Identification and Neurodevelopmental Contributions of an Autism Spectrum Disorder and Intellectual Disability Susceptibility Gene.

Over the last one and a half decades, copy number variation and whole-genome sequencing studies have illuminated the considerable genetic heterogeneity that underlies the etiologies of autism spectrum disorder (ASD) and intellectual disability (ID). These investigations support the idea that ASD may result from complex interactions between susceptibility-related genetic variants (single nucleotide variants or copy number variants) and the environment. This review outlines the identification and neurobiological characterization of two such genes located in Xp22.

Evaluating urban greening scenarios for urban heat mitigation: a spatially explicit approach.

Urban green infrastructure, especially trees, are widely regarded as one of the most effective ways to reduce urban temperatures in heatwaves and alleviate the adverse impacts of extreme heat events on human health and well-being. Nevertheless, urban planners and decision-makers are still lacking methods and tools to spatially evaluate the cooling effects of urban green spaces and exploit them to assess greening strategies at the urban agglomeration scale. This article introduces a novel spatially explicit approach to simulate urban greening scenarios by increasing the tree canopy cover in the existing urban fabric and evaluating their heat mitigation potential.

AUSTWICKIA CHELONAE IN A WILD GOPHER TORTOISE (GOPHERUS POLYPHEMUS) AND EVIDENCE OF POSITIVE SELECTION ON THE DIPHTHERIA-LIKE TOXIN GENE.

Austwickia (Dermatophilus) chelonae is a filamentous, Gram-positive Actinobacteria in the Dermatophilaceae family. It has caused fatal granulomatous disease in diverse captive reptile species on three continents, but its presence in wild or free-ranging populations was unknown. An adult female gopher tortoise (Gopherus polyphemus) was presented euhydrated, but cachectic and infested with ticks, with two firm, encapsulated masses over the cranioventral neck and right stifle.

Intensity and mode of reproduction are affected by flooding and light availability.

We studied the impact of flooding and light availability gradients on sexual and asexual reproduction in (Walt.) Blume, an endangered shrub found in floodplain forests of the Mississippi Alluvial Valley (MAV), USA. A water impoundment facility was used to control the duration of soil flooding (0, 45, or 90 days), and shade houses were used to control light availability (high = 72%, intermediate = 33%, or low = 2% of ambient light) received by established on native soil of the MAV.