A micromethod for determination of plasma pyridoxal phosphate and its use in assessment of storage stability of the vitamer.

A widely used macromethod employing tyrosine apodecarboxylase for measurement of pyridoxal phosphate (PLP) concentration in 0.5-1.0-ml plasma was modified to a microscale utilizing 0.

Improving the in vitro protein digestibility of sorghum with reducing agents.

We have shown in previous reports that cooked sorghum protein is less digestible than other cooked cereal proteins. The pepsin-indigestible proteins in sorghum were found to be mainly prolamin proteins. Cooking sorghum in the presence of 2-mercaptoethanol increased protein digestibility (in vitro with pepsin or trypsin/chymotrypsin) to a level comparable with other cereals.

Digestibility and utilization of protein and energy from Nasha, a traditional Sudanese fermented sorghum weaning food.

Whole grain sorghum flour was fermented into Nasha, a traditional Sudanese food, and freeze-dried or drum-dried. It was cooked and fed to convalescent malnourished infants and small children as 61% of total diet calories and all of 6.4% protein calories, with (Lys+) and without lysine supplementation to 3% of protein.

Analysis of B-6 vitamers in human milk by reverse-phase liquid chromatography.

A simple, accurate reverse-phase high performance liquid chromatography (RPLC) method was introduced for the analysis of B-6 vitamers in human milk. The assay consisted of a phosphate buffer (pH 2.9) delivered isocratically through 5 micron ODS column packing, followed by post-column bisulfite derivatization to enhance the detection of PLP (and to a minor extent PL).

Effect of maternal vitamin B-6 restriction on pyridoxal phosphate concentrations in developing regions of the central nervous system in rats.

The effect of maternal vitamin B-6 deficiency on concentrations of pyridoxal phosphate (PLP) within four different regions of the central nervous system (CNS) of progeny, the corpus striatum (CS), hypothalamus (H), cerebellum (C), total brain and first cervical segment (C-1) of the spinal cord was determined at 7, 15, 21 and 50 days of age. PLP concentrations in each CNS region, as well as in total brain at 15, 21 and 50 days postnatally, paralleled the level of vitamin B-6 in the deficient (0.6, 0.