Hospital-acquired infections involving carbapenem-resistant Acinetobacter baumannii (A. baumannii) and extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae pose significant challenges in the intensive care units. The lack of novel antimicrobial drugs amplifies the urgency to explore innovative management strategies.
View Article and Find Full Text PDFUnlabelled: This study focused on optimizing the fermentation-based production of Exopolysaccharides (EPS) from F58 initially isolated from traditional Moroccan Jben, a fresh goat cheese. Using the central composite design, yeast extract, MnSO, and time affect EPS concentration. The highest experimental and predicted EPS production yields were 2.
View Article and Find Full Text PDFPathogenic bacterial biofilms present significant challenges, particularly in food safety and material deterioration. Therefore, using Enterococcus mundtii A2, known for its antagonistic activity against pathogen adhesion, could serve as a novel strategy to reduce pathogenic colonization within the food sector. This study aimed to investigate the biofilm-forming ability of E.
View Article and Find Full Text PDFEnterococcus species are commensal organisms of the gastrointestinal tract and can also be isolated from traditional food products. They are used as probiotics in animals and less often in humans. This study aimed to investigate the antibacterial and anti-adhesive activities of twelve food-origin Enterococcus spp.
View Article and Find Full Text PDFThis study aimed to assess, for the first time, the anti-adhesion and antibiofilm effects of Humbert essential oil against multidrug resistant . Scanning electron microscope was used for visualizing its antibiofilm activity and the effect of this oil on surface physicochemical parameters was examined as a possible anti-adhesive target. Chemical analysis of essential oil showed a high content of carvacrol composition (79.
View Article and Find Full Text PDFThis work describes the spatiotemporal analysis of microplastics (MPs) pollution in sediments collected on a high urbanized beach located in Taghazout coast, central Atlantic part of Morocco. The study area is mainly composed of moderately well-sorted fine sands with an average density of MPs that ranged between 915 MPs/kg in 2018 and 1448 MPs/kg in 2019. The most polluted sites were in the south part of Taghazout coast, close to facilities of where beachgoers are often found.
View Article and Find Full Text PDFThe biofilm formation on the surfaces which are in direct contact with food products might lead to their contamination and, consequently, present serious health problems for the consumers. The goals of the present work were to study biofilm formation on two granites and to investigate the efficiency of sodium hypochlorite (NaCLO) against the same biofilm formed on these substrata using the plate count method (PCM) and epifluorescence microscopy (EP). More biofilm cells adhered to Rosa Porrino than Gris Pinhel, and the PCM method indicated that NaCLO was efficient against the biofilm installed on the Gris Pinhel at the concentration of 1.
View Article and Find Full Text PDFThis study reports the occurrence of different Vibrio and Salmonella species in 52 samples of Mytilus galloprovincialis collected from four sites along the Atlantic coast between Agadir and Essaouira (Anza, Cap Ghir, Imssouane and Essaouira). The level of Escherichia coli (E. coli) was also determined to evaluate the degree of microbial pollution in the investigated areas.
View Article and Find Full Text PDFColloids Surf B Biointerfaces
February 2013
The first step in the biofilm formation is the bacterial attachment to solid surfaces, which is dependent on the bacteria cell surface physico-chemical properties. The purpose of this work was to analyze the effect of pH on the physicochemical cell surface properties of Acinetobacter baumannii by two different methods. The cell surface properties were evaluated using the microbial adhesion to solvents method (MATS) and contact angle measurements (CAM).
View Article and Find Full Text PDFInitial microbial adhesion to surfaces is a complicated process that is affected by a number of factors. An important property of a solution that may influence adhesion is pH. The surface properties of the cedar wood were characterized by the sessile drop technique.
View Article and Find Full Text PDFA simple and rapid method, Microbial adhesion to hexadecane, for estimating the cell surface charge is proposed. This method is based on the determination of cell affinity to hexadecane at low ionic strength and at high ionic strength. The difference between these two affinities can provide the relative cell surface charge.
View Article and Find Full Text PDFThe initial interaction between microorganisms and substrata is mediated by physicochemical forces, which in turn originate from the physicochemical surface properties of both interacting phases. In this context, we have determined the physicochemical proprieties of all microorganisms isolated from cedar wood decay in an old monument at the Medina of Fez-Morocco. The cedar wood was also assayed in terms of hydrophobicity and electron dono-r-electron acceptor (acid-base) properties.
View Article and Find Full Text PDFThe electron donor-electron acceptor (acid-base properties) of cell surfaces of a series of bacteria were determined by two methods, namely, Microbial Adhesion to Solvents (MATS) and Contact Angle Measurements (CAM) combined with equation of Van Oss. The efficiency of these two methods was then compared. Pseudomonas aeruginosa ATCC 27853, Bacillus subtilis ILP 142B, Staphylococcus aureus ATCC 25923 and four Escherichia coli strains including HB101, AL52, O128B12 and ATCC 25922, acid-base properties were examined under the two different conditions mentioned above.
View Article and Find Full Text PDFEscherichia coli surface characteristics including hydrophobicity, electrophoretic mobility and surface functional groups' composition were investigated. These characteristics were determined respectively by water contact angle measurements, microelectrophoresis and x-ray photoelectron spectroscopy (XPS). The relation between the physicochemical properties and functional groups' composition was also examined.
View Article and Find Full Text PDFThe formation of biofilm is a universal bacterial survival strategy. Biofilms occur on inert and living support in the natural environment and in industrial installations. This microenvironment leads to the horizontal transfer of genetic material between bacteria by physical contact.
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