Invitro impact of a combination of red and infrared LEDs, infrared laser and magnetic field on biomarkers of oxidative stress and hemolysis of erythrocytes sampled from healthy individuals and diabetes patients.

Aims: Low-intensity infrared laser irradiation with output emissions of the laser and LED for in vitro irradiation of plasma and erythrocyte samples collected from healthy individuals and diabetes mellitus (DM) patients was used in the current study.

Methods: The generated emission was in the range 0.85-0.

Photoperiod-dependent changes in oxidative stress markers in the blood of Shetland pony mares and stallions involved in recreational horseback riding.

The objective of the current study was to determine the photoperiod-induced variations and the impact of exercise on oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), aldehydic (AD) and ketonic (KD) derivatives of oxidatively modified proteins (OMP), total antioxidant capacity (TAC), and activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)] and biomarkers of metabolic alterations [glucose, urea, and uric acid and the activity of lactate dehydrogenase (LDH)] in the blood of Shetland pony mares and stallions involved in recreational horseback riding. Twenty-one healthy adult Shetland ponies (11 mares and 10 stallions) aged 6.5 ± 1.

Do the diverse environments of Baltic coastal zone affect hematological and biochemical alterations in the blood of mute swans (Cygnus olor)?

Based on studies of the wintering population of mute swans in large urban agglomerations, it is possible to identify and infer the impact of environmental pollution in coastal regions near Baltic Sea on the physiological and biochemical changes in the blood of waterfowls. Hematological and biochemical changes in the blood in relation to chemical elements in their feathers are a useful tool for general ecophysiological conclusions. Hematological changes and blood chemistry in Mute Swan Cygnus olor is particularly environmentally dependent, therefore we examined hematological picture (red blood cells RBC, white blood cells WBC, heterophiles, eosinophils, basophils, monocytes, lymphocytes, hemoglobin, heterophile to lymphocyte ratio H/L, biochemical indicators (glucose, uric acid, total cholesterol, triglycerides, calcium, inorganic phosphorus, magnesium, total protein), stability of erythrocyte membranes, concentration of Ca, P, Mg in the blood, concentration of Al, Zn, Rh, Cu, Ru, Fe, Pb in feathers, in wintering population of 172 mute swans of different age (juvenile, adult) and gender, in three coastal types of areas of Southern Polish zone of Baltic Sea (Słupsk, Gdynia, Sopot).

Enzymatic Antioxidant Defense and Polymorphic Changes in Male Infertility.

The intensification of oxidative stress and destabilization of the antioxidative defenses of an organism is a consequence of many environmental factors. We considered aspects conditioning male reproductive potential and the functionality of enzymatic antioxidative mechanisms, i.e.

Effects of melatonin and metformin in preventing lysosome-induced autophagy and oxidative stress in rat models of carcinogenesis and the impact of high-fat diet.

Imbalanced glucose tolerance and insulin resistance remain important as high cancer risk factors. Metformin administration to diabetic patients may be associated with a reduced risk of malignancy. The combined effects of the hormone melatonin and metformin in oncology practice have shown positive results.

Biomarkers of oxidative stress, metabolic processes, and lysosomal activity in the muscle tissue of the great tit (Parus major) living in sodium industry and agricultural areas in Inowrocław region (central part of northern Poland).

An region of ecological hazard (EHR) is an area where, as a result of intensive human economic activity, degradation of components of the natural environment has taken place, leading to the deterioration of the ecological balance. EHR management is a globalised ever-increasing challenge. To eliminate the hazardous effect of these pollutants, research has been accelerated worldwide.

Habitat-, age-, and sex-related alterations in oxidative stress biomarkers in the blood of mute swans (Cygnus olor) inhabiting pomeranian coastal areas (Northern Poland).

The mute swan (Cygnus olor) can be considered a representative species of birds associated with the aquatic environment and responding very clearly to changes in the environment. Assuming that the condition of the mute swan population well reflects the state of the environment, this species was used in our research as a bioindicative species. Thus, the aim of our study was to elucidate the association between metal contents in soil samples collected from a habitat of mute swans and element contents in their feathers as well as the levels of biomarkers of lipid peroxidation, oxidatively modified proteins, and total antioxidant capacity in the blood of mute swans living in three agglomerations in coastal areas in the southern part of the Baltic Sea (Pomeranian region, northern Poland).

A Comparison of Oxidative Stress Biomarkers in the Serum of Healthy Polish Dairy Goats with Those Naturally Infected with Small Ruminant Lentivirus in the Course of Lactation.

The present study examines the effects of natural infection by small ruminant lentivirus (SRLV) in the two most common goat breeds in Poland, i.e., Polish white improved and polish fawn improved.

Dietary nutrients and health risks from exposure to some heavy metals through the consumption of the farmed common carp ().

Common carp () is one of the most important cultured fish species in Poland. The aims of our study were to determine the concentration of essential minerals in the muscle tissue of carp obtained from a pond culture and to compare the content of these minerals with the physiological norms of nutrition for the Polish population, Recommended Dietary Allowances (RDA), and Estimated Average Requirements (EAR). The non-carcinogenic and carcinogenic risk by assessment of the Estimated target hazard quotients (THQ), total target hazard quotients (ΣTHQ), and carcinogenic risk were also studied.

Photoperiod-induced alterations in biomarkers of oxidative stress and biochemical pathways in rats of different ages: Focus on individual physiological reactivity.

Effects of photoperiodicity caused by both the age and individual physiological reactivity estimated by resistance to hypobaric hypoxia on the levels of lipid peroxidation, protein oxidation (aldehydic and ketonic derivatives), total antioxidant capacity, activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase), and biochemical parameters of aerobic and anaerobic pathways in hepatic tissue depending on the blood melatonin level were studied. The study was carried out on 96 6- and 21-month-old male rats divided into hypoxia resistance groups (LR, low resistance, HR, high resistance). The analyses were conducted at four photoperiods: winter (January), spring (March), summer (July), and autumn (October).

Changes in energetic metabolism and lysosomal destruction in the skeletal muscle and cardiac tissues of pigeons (Columba livia f. urbana) from urban areas of the northern Pomeranian region (Poland).

The aim of the present study was to evaluate the biochemical responses of the skeletal muscle and cardiac tissues of the urban pigeon as a bioindicator organism tested in diverse environments (Szpęgawa as a rural environment and Słupsk as an urban environment, Pomeranian Voivodeship, northern Poland), resulting in changes in the level of lipid peroxidation at the initial and final stages of this process and the activities of Krebs cycle enzymes (succinate dehydrogenase, pyruvate dehydrogenase, isocitrate dehydrogenase, and alfa-ketoglutarate dehydrogenase). Szpęgawa village was chosen due to the intensive use of the European motorway A1 with significant traffic and pollution levels. The concentration of Pb was higher in the soil and feathers of pigeons nesting in the polluted areas (Szpęgawa).

Studies on The Inhibitory Properties of Leaf Ethanolic Extracts Obtained from () Species Against Spp. Strains.

Introduction: The most frequently isolated bacteria in Polish aquaculture are of the genus; also pathogenic to human fish consumers, they cause substantial economic losses, and require antibiotic therapy to treat. Antibiotic residues in animal-derived food provoke concern. The aim of the study was to appraise the antimicrobial activity of ethanolic extracts of plant species against strains.

Profile of Heavy Metals and Antioxidant Defense in the Muscle Tissues of Pigeons (Columba livia f. urbana) from Anthropogenically Transformed Areas in the Pomeranian Region (Northern Poland).

Pigeons can be successfully used as bioindicators of a contaminated environment. We studied the relationship between the functioning of the pro/antioxidant balance in muscle tissues (skeletal muscle and cardiac tissues) of pigeons (Columba livia f. urbana) living in areas with different levels of pollution (Pomeranian Voivodeship, Northern Poland).

Antioxidants, lysosomes and elements status during the life cycle of sea trout Salmo trutta m. trutta L.

The aim of our study was to elucidate the effects of both development stages (parr, smolt, adult, spawner), and kelt as a survival form and sex (male, female) on the functional stability of the lysosomal complex, biomarkers of oxidative stress, and element contents in the muscle tissue of the sea trout (Salmo trutta m. trutta L.) sampled in the Pomerania region (northern Poland).

Relationship Between Thyroid Hormonal Status in Patients with a Hypothyroid Form of Hashimoto's Thyroiditis and Iodine Concentrations in Drinking Water.

The current study aimed to identify correlative and regressive dependencies between the water iodine concentration and the levels of TSH (thyroid-stimulating hormone), thyroglobulin antibodies (TgAbs), and thyroid peroxidase (TPOAb) in the serum of 168 in patients (34 men and 134 women) with a hypothyroid form of Hashimoto's thyroiditis who use water from the supply network and individual wells. Based on the water iodine concentration, low and moderate degrees of iodine endemia in the location of the patients were determined. In the groups of men and women using water from different water supply sources, there were direct correlations between the water iodine concentrations and the TgAbs and TPOAb titers as well as an inverse dependence between iodine and TSH levels.

Melatonin modulates oxidative phosphorylation, hepatic and kidney autophagy-caused subclinical endotoxemia and acute ethanol-induced oxidative stress.

The study establishes a link between alcoholism, inflammation state, and melatonin synthesis. The aim of our study was to evaluate the effects of melatonin on changes in the relationships between oxygen consumption (using NADH- or FAD-generated substrates of mitochondrial respiration), activities of lysosomal enzymes, such as alanyl aminopeptidase (AAP), leucyl aminopeptidase (LAP), β-N-acetylglucosaminidase (NAG), and acid phosphatase (AcP), biomarkers of oxidative stress estimated by the 2-thiobarbituric acid reactive substance (TBARS) level as a biomarker of lipid peroxidation, carbonyl derivatives as biomarkers of oxidatively protein damage, and biomarkers of energy metabolism during acute ethanol-induced stress (AES), and a low-dose lipopolysaccharide (LPS)-induced inflammatory responses in mice. Biochemical assays of lysosomal enzymes, biomarkers of oxidative stress, and parameters of energy metabolism (activities of alanine- and aspartate aminotransferases, succinate dehydrogenase, levels of lactate and pyruvate) were carried out in eight groups: 1) untreated control, 2) melatonin treatment (Mel, 10 mg/kg b.

Melatonin maintains the function of the blood redox system at combined ethanol-induced toxicity and subclinical inflammation in mice.

Background: The goal of this study was to assess the effect of melatonin on blood redox systems in mice simultaneously exposed to ethanol and low-dose lipopolysaccharide (LPS).

Methods: Oxidative stress parameters were assessed in eight groups: untreated control, melatonin (10 mg kg, 10 days), LPS (injected once intraperitoneally at a dose of 150 μg per mouse), LPS with previous melatonin treatment, acute ethanol-induced stress (AES, 0.75 g kg per day, 10 days), AES with previous melatonin treatment, LPS- and AES-induced toxicity, and melatonin treatment.

Elemental Status and Lipid Peroxidation in the Blood of Children with Endemic Fluorosis.

The study aimed to assess the levels of trace elements, minerals, and toxic elements as well as lipid peroxidation biomarkers (lipid acyl hydroperoxides, 2-thiobarbituric acid reactive substances (TBARS)) in the blood of children with chronic fluorosis from endemic fluorosis areas (Sosnivka village, Lviv region, western Ukraine). The results were compared with healthy children from Staryi Sambir (Lviv region, western Ukraine), whose drinking water contained permissible levels (< 1 ppm) of fluoride. Thirty-one children from the Sosnivka village in the Lviv region, including 16 females and 15 males aged 7-10 years, with clinically diagnosed fluorosis, were recruited for the study.

Melatonin and alcohol-related disorders.

This review concerns the current knowledge of melatonin and alcohol-related disorders. Chronobiological effects of ethanol are related to melatonin suppression and in relation to inflammation, stress, free radical scavenging, autophagy and cancer risk. It is postulated that both alcohol- and inflammation-induced production of reactive oxygen species (ROS) alters cell membrane properties leading to tissue dysfunction and, subsequent further ROS production.

Evaluation of oxidative stress markers in the heart and liver of rainbow trout (Oncorhynchus mykiss walbaum) exposed to the formalin.

The aim of this study was to examine change in lipid and protein oxidation biomarkers, transamination enzymes and lactate dehydrogenase activities, lactate and pyruvate levels in liver and heart tissue of rainbow trout (Oncorhynchus mykiss Walbaum) that was exposed to formalin baths. Increase of 2-thiobarbituric acid reactive substances and carbonyl derivatives of protein oxidative destruction was noticed only in cardiac tissue of formalin-exposed fish. Activity of lactate dehydrogenase and lactate level in the cardiac tissue were elevated, indicating active glycolysis.

Tissue-specific responses of oxidative stress biomarkers and antioxidant defenses in rainbow trout Oncorhynchus mykiss during a vaccination against furunculosis.

The present study was conducted to evaluate the effects of vaccination against furunculosis on responses of oxidative stress and antioxidant defenses in rainbow trout Oncorhynchus mykiss muscle, gills, liver, and brain tissues. The oxidative stress markers (malondialdehyde and carbonyl derivatives of protein oxidative destruction levels), antioxidant defenses (superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase), and total antioxidant capacity in different tissues of rainbow trout were measured. Our data showed that exposure of trout to vaccine against furunculosis produced changes (either increase or decrease) in oxidative stress and antioxidant enzymes responses, and these responses showed marked organ differences, associated with tissue patterns.

The effects of intermittent hypoxia training on mitochondrial oxygen consumption in rats exposed to skeletal unloading.

Intermittent hypoxia training (IHT) may reduce the oxidative stress-induced damage caused by extreme influences such as ischemia, exhaustive physical exercise, acute hypoxia, and stress. The aim of the present study is to investigate the effects of IHT on hepatic mitochondrial oxygen consumption, lipid peroxidation, and selected biochemical parameters used as diagnostic tools in a skeletal unloading model in rats. Our data showed that the IHT method significantly improved liver tolerance of unloading by reorganizing mitochondrial energy metabolism due to NADH-dependent oxidation.

Pollution-induced oxidative stress and biochemical parameter alterations in the blood of white stork nestlings Ciconia ciconia from regions with different degrees of contamination in Poland.

This paper aimed to illustrate the most reliable biomarkers to detect pollution-related oxidative stress in white stork nestlings in polluted (from copper manufacture), suburban and Odra meadows (as a control area) areas . Lipid peroxidation (thiobarbituric acid reactive substances content, TBARS) and oxidative modified protein levels (stable 2,4-dinitrophenyl hydrazine derivates of the carbonyl groups) were used as indicators of the oxidative stress, as well as other selected biochemical parameters, which are used as diagnostic tools in avian medicine (alanine and aspartate aminotransferases, lactate dehydrogenase activities, lactate and pyruvate concentrations). It was found that the blood of chicks from the polluted area was more susceptible to oxidative stress due to lipid peroxidation and protein oxidation, manifested as protein carbonyls, the elevation of alanine and aspartate aminotransferases activities, and lactate and pyruvate concentrations than those of chicks from suburban and Odra meadows areas.

Ecophysiological determinations of antioxidant enzymes and lipoperoxidation in the blood of White Stork Ciconia ciconia from Poland.

The aim of this study was to investigate the activity of antioxidant enzymes in the blood of White Stork Ciconia ciconia chicks (aged 19-54 days) in Poland in 2006. We took under consideration superoxide dismutase (SOD), catalase (CAT), ceruloplasmine (CP), glutathione peroxidase (GPx), glutathione reductase (GR) and the content of thiobarbituric acid-reactive substances (malondialdehyde) in polluted (copper manufacture), suburban areas, at the Odra meadows, and at swamps near Baltic Sea in the Pomeranian region. We examined the levels of Na, K, Ca, Mg, Fe, Zn, Cu, Mn, Co, Cd, and Pb and compared ecophysiological determinations for developing storks.

The impact of element-element interactions on antioxidant enzymatic activity in the blood of white stork (Ciconia ciconia) chicks.

The aim of this work was to determine interrelationships among macroelements Na, K, Ca, Mg, and Fe, microelements Zn, Cu, Mn, and Co, and toxic heavy metals Pb and Cd in the blood of white stork Ciconia ciconia, during postnatal development, in different Polish environments, and their impact on the activity of antioxidant enzymes. We considered the content of thiobarbituric acid-reactive substances (TBARSs), i.e.