[Effect of Sonic Hedgehog Signal Pathway Inhibitor Jervine on Myelodysplastic Syndromes MUTZ-1 Cells].

Objective: To study the effect of SMO inhibitor (Jervine) on proliferation, apoptosis and cell cycle of MDS cell line MUTZ-1, and its mechanism.

Methods: The effect of different concentrations Jervine on proliferation of MUTZ-1 cells was detected by CCK-8 method. Apoptosis and cell cycle of MUTZ-1 cells were detected by flow cytometry.

[Expression Level and Clinical Significance of Gli1 in Patients with Myelodysplastic Syndrome].

Objective: To study the expression level and clinical significance of Gli1 gene in patients with myelodysplastic syndrome(MDS).

Methods: The positive rate of bone marrow CD34 cells was detected by flow cytometry in 53 patients with MDS.Magnetic beads were used to separate CD34 cells.

Percentages of PD-1CD4T cells and PD-L1DCs are increased and sPD-1 level is elevated in patients with immune thrombocytopenia.

The present study is to measure the expression of programmed death (PD)-1 / programmed death ligand-1 (PD-L1) negative costimulatory molecules, soluble format sPD-1 in patients with immune thrombocytopenia (ITP), and to investigate their correlation with the secretion of cytokines. A total of 35 patients with ITP were included in the present study. Twenty healthy subjects who received physical examination at our hospital were included as control group.

The effect of single course high dose dexamethasone on CD28/CTLA-4 balance in the treatment of patients with newly diagnosed primary immune thrombocytopenia.

To evaluate the effect of a single course of high dose dexamethasone (HD-DXM) on CD28 and CTLA-4 expression in patients with newly-diagnosed primary immune thrombocytopenia (ITP). Twenty-8 ITP patients (18 females and 10 males, age range 18-65 years, median age 38.5 years) enrolled in this study and 26 healthy volunteers (19 women and 7 men, age range 16-66 years, median age 37 years) served as a control group.

Polymorphic variation of inflammation-related genes and risk of non-Hodgkin lymphoma for Uygur and Han Chinese in Xinjiang.

Polymorphisms of inflammation-related genes have been found to be associated with non-Hodgkin lymphoma (NHL) or some of its subtypes, but only a few relevant data have been reported in China. In this study, the Snapshot method was used to assess genetic variation; a total of 14 single nucleotide polymorphisms (SNPs) for 6 inflammatory factors in 157 NHL cases (64 Uygur ethnic subjects, 93 Han Chinese) and 435 controls (231 Uygur and 204 Han Chinese) were studied from the Xinjiang province of China. Haplotype distribution was estimated using PHASE 2.

[CD28 expression on CD4(+);T cells and its relationship with IFN-γ/IL-10 ratio in patients with primary immune thrombocytopenia].

Objective: To investigate CD28 expression on CD4(+);T cells and its relationship with IFN-γ/IL-10 ration in patients with primary immune thrombocytopenia (ITP) before and after treatment.

Methods: The expression of CD4(+);CD28(+); was detected by flow cytometry, the levels of IFN-γ and IL-10 were determined by double-antibody sandwich ELISA and platelet count was tested by blood cells automatical counter in peripheral blood of 30 patients with ITP before and after glucocorticoid treatment and 26 cases of normal controls. Then the correlations between the outcomes were analyzed.

[Application of karyotypic analysis in myelodysplastic syndrome].

Objective: To explore the applications of karyotypic analysis in the diagnosis and prognosis of myelodysplastic syndrome (MDS).

Methods: Chromosomal analysis was performed with short-term cell cultures and R-banding techniques in 129 MDS patients. And the technique of FISH was employed for clinical follow-ups in 28 MDS patients.

[Detection and clinical significance of Th1/Th2 cytokines in patients with idiopathic thrombocytopenic purpura].

Aim: To observe Th1 (IL-2 and IFN-γ) and Th2 (IL-4, IL-10) cytokine changes in patients with idiopathic thrombocytopenic purpura (ITP) and explore the correlation between ITP development and Th1/Th2.

Methods: A total of 30 patients with ITP and 26 healthy volunteers were enrolled in this study. Serum levels of IL-2, IFN-γ, IL-4, and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA) before and after treatment.

[Expression of CD4+ CD25+ regulatory T cells and TGF-ß1 in patient with idiopathic thrombocytopenic purpura].

Aim: To investigate the expression rate of CD4+ CD25+ regulatory T cells and TGF-ß1 in peripheral blood of the patients with idiopathic thrombocytopenic purpura (ITP), and the role they play in the pathogenesis of ITP.

Method: The population of CD4+ CD25+ regulatory T cells in peripheral blood of 31 patients and 25 healthy donors was evaluated by flow cytometry, ELISA was used to test the level of TGF-ß1 in blood serum, and analysed the correlation between levels of CD4+ CD25+ regulatory T cells and TGF-ß1.

Results: ITP patients had a lower proportion of CD4+ CD25+ regulatory T cells than the healthy donors (P<0.

[Comparison of immunological reconstitution and related complications after HLA-matched and HLA haploidentical peripheral blood hematopoietic stem cell transplantation].

Objective: To explore the difference of immune function and relationship with main complications after HLA-matched and HLA haploidentical allogeneic peripheral blood hematopoietic stem cell transplantation (allo-PBSCT).

Methods: Sixty-seven patients undergoing HLA-matched (n = 33) or HLA haploidentical (n = 34) allo-PBSCT during the same time period in our hospital from June 2004 to December 2007 were included in this study. Indirect immunofluorescence assay was employed to detect lymphocyte subsets before transplantation and on month 1, 3, 6, 12 and 18 after transplantation and the lymphocyte subsets of 100 healthy people were used as normal control.

[Non-T-cell depleted HLA haploidentical peripheral blood stem cell transplantation for hematological malignancies: report of 36 cases].

Objective: To analyze the clinical outcome of human leukocyte antigen (HLA) haploidentical peripheral blood stem cell transplantation (PBSCT) from related donors for hematological malignancies.

Methods: Thirty-six patients with hematological malignancies, with a median age of 25 (11-48) years, were transplanted with PBSC from an HLA-haploidentical family donors: 7 were 1 locus mismatched and 29 were 2-3 loci mismatched. The recipients received myeloablative conditioning regimen, in combination with different immunosuppressants according to the degree of HLA disparity followed by non-T-cell depleted PBSCT.

[Retinoic acid in treating acute promyelocytic leukemia with hyperleukocytosis and its therapeutic strategy].

In order to investigate the occurrence of hyperleukocytosis in treating acute promyelocytic leukemia (APL) patients with all trans retinoic acid (ATRA) and to explore the influence of the level of leucocyte on curative effect of ATRA, the APL patients were divided into three different groups according to the count of leucocyte in peripheral blood. Patients with WBC count less than 30x10(9)/L were administered with ATRA alone (the first group), patients with WBC count more than 30x10(9)/L were administered with ATRA alone (the second group) and patients with WBC count more than 30x10(9)/L were treated with ATRA+cytotoxic drugs (the third group). The results showed that hyperleukocytosis were found in 23 out of 39 patients (58.

[Early prediction of efficaciousness after first course of chemotherapy in de novo acute leukemia].

To achieve the complete remission (CR), acute leukemia (AL) patient must get through the period of myeloid ablation after chemotherapy that the white blood cell (WBC) count in peripheral blood decreases rapidly. To observe the relationship of WBC count with therapeutic effectiveness after chemotherapy in previously untreated AL, eighty cases of previously untreated acute leukemia who took the first induction chemotherapy course were analyzed. Blood routine was carried out 2 to 3 times every week while the bone marrow pictures on 12th and 20th day after chemotherapy were observed.