and Anti-Picornavirus Triterpene Alkanoic Acid Ester from Saudi Collection of Decne.

The total alcohol extract obtained from the aerial parts of and fractions of the liquid-liquid fractionation process were tested against picornavirus-causing foot-and-mouth disease (FMD) based on the traditional use of the plant in Saudi Arabia. The most active petroleum ether soluble fraction was subjected to chromatographic purification, and nine compounds were isolated, identified using various chemical and spectroscopic methods, and tested for their anti-viral potential. The new ester identified as -Amyrin 3-(3'R-hydroxy)-hexadecanoate () was the most active compound with 51% inhibition of the viral growth and was given the name Rhazyin A.

Forecasting of potential anti-inflammatory targets of some immunomodulatory plants and their constituents using in vitro, molecular docking and network pharmacology-based analysis.

Most synthetic immunomodulatory medications are extremely expensive, have many disadvantages and suffer from a lot of side effects. So that, introducing immunomodulatory reagents from natural sources will have great impact on drug discovery. Therefore, this study aimed to comprehend the mechanism of the immunomodulatory activity of some natural plants via network pharmacology together with molecular docking and in vitro testing.

Brain Targeting by Intranasal Drug Delivery: Effect of Different Formulations of the Biflavone "Cupressuflavone" from L. on the Motor Activity of Rats.

The polar fractions of the species are rich in bioflavonoid contents. Phytochemical study of the polar fraction of aerial parts resulted in the isolation of cupressuflavone (CPF) as the major component in addition to another two bioflavonoids, amentoflavone and robustaflavone. Biflavonoids have various biological activities, such as antioxidant, anti-inflammatory, antibacterial, antiviral, hypoglycemic, neuroprotective, and antipsychotic effects.

Discovery of potential natural dihydroorotate dehydrogenase inhibitors and their synergism with brequinar via integrated molecular docking, dynamic simulations and in vitro approach.

The critical function of dihydroorotate dehydrogenase (DHODH) in pyrimidine synthesis attracted a great interest throughout beyond decades. Inhibitors of human DHODH (hDHODH) have validated efficacy for remedy of many immunological diseases. Brequinar and leflunomide are examples of such compounds.

Determination of Colchicine in Pharmaceutical Formulations, Traditional Extracts, and Ultrasonication-Based Extracts of Pleniflorum (L.) Using Regular and Greener HPTLC Approaches: A Comparative Evaluation of Validation Parameters.

In the literature, there is a scarcity of greener analytical approaches for colchicine (CLH) analysis. As a result, efforts were made in this study to develop and validate a greener reversed-phase high-performance thin-layer chromatography (HPTLC) technique for CLH analysis in traditional extracts (TE) and ultrasonication-based extracts (UBE) of commercial Unani formulations, commercial allopathic formulations, and Pleniflorum (L.) obtained from Egypt and India.

Biologically-Guided Isolation of Natural Lead Antithyroid Drug from Medicago sativa L. Sprouts and Its Toxic Profile in Comparison with Propylthiouracil.

Hyperthyroidism is a common endocrine disorder associated with increased risk of cardiovascular complications and mortality. Although antithyroid drugs (ATDs) are approved as first line option for many hyperthyroidism cases, including pregnancy and childhood, they exert significant toxic profile. Medicago sativa L.

Chemometric evaluation of alfalfa sprouting impact on its metabolic profile using HPTLC fingerprint-efficacy relationship analysis modelled with partial least squares regression.

Sprouting is a commonly applied food processing practice specially in Western countries. Tracking the impact of sprouting of Medicago sativa L. (alfalfa) seeds on their phytochemical composition and curative efficacy was implemented in the current study.

methanolic extract improves oxidative stress-related male infertility induced in experimental animals.

Aim: This study designated to investigate and compare the therapeutic effect of methanolic extract against oxidative stress (OS)-infertility induced by naturally occurring prooxidants (gossypol) and selenium- vitamins A, C, and E (selenium-ACE) drug.

Methods: Male infertility was induced in rat by intraperitoneal injection of 5 mg/kg gossypol eight times then the treatment was carried out with 100 mg/kg ulva methanolic extract oral administration for one or two weeks, after this period OS, and male infertile markers were detected in blood and/or testes.

Results: Gossypol stimulated male infertility by increasing testicular OS markers and decreasing semen quality, hyaluronidase enzyme activity, and blood testosterone level.

Chemical composition of fennel seed extract and determination of fenchone in commercial formulations by GC-MS method.

In the present study, various phytoconstituents of methanolic extract of were identified using gas-chromatography mass spectrometry (GC-MS) method. GC-MS method was also applied for the analysis of biomarker fenchone in extract and eight different commercial formulations. The mass of prepared extract and formulations A-D and H (commercial herbal mixtures and commercial extract) used for the analysis of fenchone was 10 g.

Digitally-optimized HPTLC coupled with image analysis for pursuing polyphenolic and antioxidant profile during alfalfa sprouting.

The utilization of Folin-Ciocalteu as a post-chromatographic derivatization reagent for direct quantitative estimation of polyphenolic compounds using HPTLC coupled with image analysis was developed, validated and implemented in this study. The phenolic compounds react with Mo; the hypothesized active center in molybdotungstophosphoric heteropolyanion causing its reduction into blue colored complex. In addition, a direct TLC-DPPH assay was developed and applied to determine the correlation between the polyphenolic content and their free radical scavenging ability during the sprouting of alfalfa seeds for eleven days.

QUANTIFICATION OF GLYCYRRHIZIN BIOMARKER IN GLYCYRRHIZA GLABRA RHIZOME AND BABY HERBAL FORMULATIONS BY VALIDATED RP-HPTLC METHODS.

Background: A simple and sensitive thin-layer chromatographic method has been established for quantification of glycyrrhizin in rhizome and baby herbal formulations by validated Reverse Phase HPTLC method.

Materials And Methods: RP-HPTLC Method was carried out using glass coated with RP-18 silica gel 60 F254S HPTLC plates using methanol-water (7: 3 v/v) as mobile phase.

Results: The developed plate was scanned and quantified densitometrically at 256 nm.

Prenylated flavonoids of Erythrina lysistemon grown in Egypt.

Three prenylated flavonoid derivatives; 5,7,4'-trihydroxy-8-(3"'-methylbut-2"'-enyl)-6-(2"-hydroxy-3"-methylbut-3"enyl) isoflavone (isoerysenegalensein E), 5,7,2'-trihydroxy-4'-methoxy-5'-(3"-methylbut-2"-enyl) isoflavanone (lysisteisoflavanone), 5, 4'-dihydroxy-6-(3"'-methylbut-2"'-enyl)-2"-hydroxyisopropyl dihydrofurano [4",5":8,7] isoflavone (isosenegalensin), together with the four known flavonoids abyssinone V-4'-methylether, alpinumisoflavone, wighteone and burttinone were isolated from the stem bark of Erythrina lysistemon Hutch. (Leguminosae). Structures were elucidated by spectroscopic methods.