The prebiotic potential of dietary onion extracts: shaping gut microbial structures and promoting beneficial metabolites.

Onions are well-known vegetables that offer various health benefits. This study explores the impact of onion extracts on gut microbiome using an fecal incubation model and metabolome analysis. Fecal samples were collected from 19 healthy donors and incubated in the presence or absence of onion extracts for 24 h.

Targeted dual-receptor phage cocktail against : insights into phage-host interactions and resistance mechanisms.

Introduction: is a notorious foodborne pathogen, frequently contaminating powdered infant formula and causing life-threatening diseases in infants. The escalating emergence of antibiotics-resistant mutants has led to increased interest in using bacteriophage as an alternative antimicrobial agent.

Methods: Two phages, CR8 and S13, were isolated from feces and soil samples and their morphology, physiology, and genomics were characterized.

Efficient Biosynthesis of Theanderose, a Potent Prebiotic, Using Amylosucrase from .

The study aimed to develop an efficient bioprocess for the discovery and synthesis of theanderose by using amylosucrase from (AS). An unknown trisaccharide produced by AS was detected by high-performance anion-exchange chromatography-pulsed amperometric detection and high-performance liquid chromatography-evaporative light scattering detection, purified using medium-pressure liquid chromatography, and identified as theanderose (α-d-glucopyranosyl-(1→6)-α-d-glucopyranosyl-(1→2)-β-d-fructofuranoside) through nuclear magnetic resonance and mass spectrometry. AS synthesized theanderose with a 25.

Garlic-Induced Enhancement of : Enterotype-Specific Modulation of Gut Microbiota and Probiotic Populations.

The gut microbiome is a dynamic ecosystem crucial for maintaining its host's health by regulating various immune and metabolic functions. Since diet plays a fundamental role in shaping the gut microbiome, understanding the relationship between food consumption and microbiome structure is essential. Although medicinal plants are widely recognized for their broad health benefits, their specific impact on the gut microbiome remains unclear.

The Impact of Makgeolli Consumption on Gut Microbiota: An Enterotype-Based Preliminary Study.

Makgeolli, a traditional Korean liquor, contains components such as lactic acid bacteria and dietary fiber, which can induce changes in the gut microbiome. Since variations in microbiome responses may exist between enterotypes-classifications based on the dominant bacterial populations in the gut-we hypothesized that the consumption of makgeolli leads to enterotype-dependent differences in gut microbial structures among healthy participants. This study aimed to determine the effect of makgeolli consumption on gut microbial structures by stratifying all participants into two enterotype groups: Bacteroides-dominant type (B-type, n = 7) and Prevotella-dominant type (P-type, n = 4).

Precision Phage Cocktail Targeting Surface Appendages for Biocontrol of in Cold-Stored Foods.

is a major food-borne pathogen causing food poisoning. The use of bacteriophages as alternative biocontrol agents has gained renewed interest due to the rising issue of antibiotic-resistant bacteria. We isolated and characterized three phages targeting : SPN3US, SPN3UB, and SPN10H.

Deciphering the Impact of Defecation Frequency on Gut Microbiome Composition and Diversity.

This study explores the impact of defecation frequency on the gut microbiome structure by analyzing fecal samples from individuals categorized by defecation frequency: infrequent (1-3 times/week, = 4), mid-frequent (4-6 times/week, = 7), and frequent (daily, = 9). Utilizing 16S gene-based sequencing and LC-MS/MS metabolome profiling, significant differences in microbial diversity and community structures among the groups were observed. The infrequent group showed higher microbial diversity, with community structures significantly varying with defecation frequency, a pattern consistent across all sampling time points.

Sample Collection Methods in Upper Gastrointestinal Research.

In recent years, significant translational research advances have been made in the upper gastrointestinal (GI) research field. Endoscopic evaluation is a reasonable option for acquiring upper GI tissue for research purposes because it has minimal risk and can be applied to unresectable gastric cancer. The optimal number of biopsy samples and sample storage is crucial and might influence results.

Partial convergence of the human vaginal and rectal maternal microbiota in late gestation and early post-partum.

The human vaginal and fecal microbiota change during pregnancy. Because of the proximity of these perineal sites and the evolutionarily conserved maternal-to-neonatal transmission of the microbiota, we hypothesized that the microbiota of these two sites (rectal and vaginal) converge during the last gestational trimester as part of the preparation for parturition. To test this hypothesis, we analyzed 16S rRNA sequences from vaginal introitus and rectal samples in 41 women at gestational ages 6 and 8 months, and at 2 months post-partum.

Seasonal Changes in the Microbial Communities on Lettuce ( L.) in Chungcheong-do, South Korea.

Lettuce is one of the most consumed vegetables worldwide. However, it has potential risks associated with pathogenic bacterial contamination because it is usually consumed raw. In this study, we investigated the changes in the bacterial community on lettuce ( L.

β-Carotene suppresses cancer cachexia by regulating the adipose tissue metabolism and gut microbiota dysregulation.

Cancer cachexia is a metabolic disease affecting multiple organs and characterized by loss adipose and muscle tissues. Metabolic dysregulated of adipose tissue has a crucial role in cancer cachexia. β-Carotene (BC) is stored in adipose tissues and increases muscle mass and differentiation.

Bifidobacterium bifidum BGN4 and Bifidobacterium longum BORI promotes neuronal rejuvenation in aged mice.

An increasing number of studies have indicated that alterations in gut microbiota affect brain function, including cognition and memory ability, via the gut-brain axis. In this study, we aimed to determine the protective effect of Bifidobacterium bifidum BGN4 (B. bifidum BGN4) and Bifidobacterium longum BORI (B.

Applicability Evaluation of Male-Specific Coliphage-Based Detection Methods for Microbial Contamination Tracking.

Outbreaks of food poisoning due to the consumption of norovirus-contaminated shellfish continue to occur. Male-specific (F+) coliphage has been suggested as an indicator of viral species due to the association with animal and human wastes. Here, we compared two methods, the double agar overlay and the quantitative real-time PCR (RT-PCR)-based method, for evaluating the applicability of F+ coliphage-based detection technique in microbial contamination tracking of shellfish samples.

Administration of BGN4 and BORI Improves Cognitive and Memory Function in the Mouse Model of Alzheimer's Disease.

Recent evidence indicates that gut microbiota could interact with the central nervous system and affect brain function, including cognition and memory. In this study, we investigated whether BGN4 ( BGN4) BORI ( BORI) alleviated the pathological features in a mouse model of Alzheimer's disease (AD). Administration of BGN4 and BORI effectively suppressed amyloidosis and apoptotic processes and improved synaptic plasticity by ameliorating the neuroinflammatory response and BDNF expression.

Complete genome sequence of K_EC180, a bacterium producing shiga-like toxin isolated from swine feces.

normally colonizes the lower intestine of animals and humans, but some serotypes are foodborne pathogens. The K_EC180 was isolated from swine feces that were collected from a weaner pig. In this genome announcement, K_EC180 was sequenced using PacBio RS II and Illumina NextSeq 500 platforms.

Complete genome sequence of strain K_SA184, multidrug resistance bacterium isolated from lamb ().

is a representative foodborne pathogen in the world. The strain K_SA184 was isolated from the lamb (), which was collected from a local traditional market in South Korea. In this study, the strain K_SA184 was sequenced using PacBio RS II and Illumina NextSeq 500 platforms.

Geranii Herba as a Potential Inhibitor of SARS-CoV-2 Main 3CL, Spike RBD, and Regulation of Unfolded Protein Response: An In Silico Approach.

Background: Since the first patient identified with SARS-CoV-2 symptoms in December 2019, the trend of a spreading coronavirus disease 2019 (COVID-19) infection has remained to date. As for now, there is an urgent need to develop novel drugs or vaccines for the SARS-CoV-2 virus.

Methods: Polyphenolic compounds have potential as drug candidates for various diseases, including viral infections.

Delayed Establishment of Gut Microbiota in Infants Delivered by Cesarean Section.

The maternal vaginal microbiome is an important source for infant gut microbiome development. However, infants delivered by Cesarean section (CS) do not contact the maternal vaginal microbiome and this delivery method may perturb the early establishment and development of the gut microbiome. The aim of this study was to investigate the early gut microbiota of Korean newborns receiving the same postpartum care services for two weeks after birth by delivery mode using fecal samples collected at days 3, 7, and 14.

Maternal H. pylori is associated with differential fecal microbiota in infants born by vaginal delivery.

Helicobacter pylori colonization may affect the mucosal immune system through modification of microbiota composition and their interactions with the host. We hypothesized that maternal H. pylori status affects the maternal intestinal microbiota of both mother and newborn.

Anti-methanogenic effect of rhubarb ( spp.) - An docking studies on methyl-coenzyme M reductase (MCR).

The present study explored anti-methanogenic properties of rhubarb compounds using analysis on methyl-coenzyme M reductase (MCR) for identifying its anti-methanogen mechanism. To identify pharmacokinetics of 35 compounds from rhubarb, molecular docking and ADME analysis were performed against MCR using AutoDockVina, FAFDrugs3 and PROTOX programs. Docking results successfully indicated three possible candidate compounds 9,10-anthracenedione, 1,8-dihydroxy-3-methyl (-6.

Microbiota Analysis for the Optimization of Isolation From Chicken Carcasses Using Selective Media.

Since contaminated poultry meat is the major source of transmitting to humans, the isolation of from poultry carcasses is frequently performed in many countries as a baseline survey to ensure food safety. However, existing isolation methods have technical limitations in isolating this fastidious bacterium, such as a growth competition with indigenous bacteria in food samples. In this study, we compared the differences in microbiota compositions between Bolton and Preston selective media, two most common selective media to isolate , and investigated how different microbiota compositions resulting from different enrichment methods may affect isolation frequencies.

Author Correction: Differences in the fecal microbiota of neonates born at home or in the hospital.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.

Differences in the fecal microbiota of neonates born at home or in the hospital.

Research on the neonatal microbiome has been performed mostly on hospital-born infants, who often undergo multiple birth-related interventions. Both the hospital environment and interventions around the time of birth may affect the neonate microbiome. In this study, we determine the structure of the microbiota in feces from babies born in the hospital or at home, and from vaginal samples of their mothers.