Microelectrophoretic application of kainic acid into the globus pallidus: disturbances in feeding behavior.

Body weight changes, food and water intake, and sensorimotor disturbances of male rats were studied after bilateral kainic acid-(KA) induced lesions of the globus pallidus (GP). To minimize the extent of damages, KA was applied electrophoretically by means of glass micropipettes (tip diameter of the pipettes was 10-15 microns). The neuron-specific damages of the GP resulted in aphagia and adipsia and rapid body weight decrease.

Stabilization of the oxy form of tyrosinase by a single conservative amino acid substitution.

Asp-208 of Streptomyces glaucescens tyrosinase (an invariant residue in the CuB-binding region of tyrosinases and haemocyanins) was conservatively substituted by glutamic acid. Although having little effect on spectroscopic or kinetic properties of the enzyme, the mutation greatly decreased the lability of Cu-bound O2. A rationalization for these results is given, based on the crystal structure of Panuliris interruptus haemocyanin in the conserved CuB-binding region.

Feeding and body weight regulation after 6-OHDA application into the preoptic area.

Our previous results showed that neurochemical destruction of the amygdaloid terminal field of the mesolimbic dopaminergic system caused disturbances in body weight regulation and feeding. In the present experiments, it was studied whether 6-hydroxydopamine (6-OHDA)-induced bilateral lesions of the mesolimbic dopaminergic system in the lateral preoptic area produce similar symptoms in rats. To enhance the selectivity of the neurotoxin, 6-OHDA was also used after desmethylimipramine (DMI) premedication.

[Hepatitis C virus antibody in Hungarian blood donors, persons at risk and patients with post-transfusion hepatitis].

The authors screened blood donors, persons at high risk of infection and patients with post-transfusion hepatitis by the use of the new hepatitis C antibody test. 1.7% of persons donating blood acceptable for use according to current criteria were found positive.

Albino mutants of Streptomyces glaucescens tyrosinase.

Site-directed mutagenesis was used to determine the functional role of several residues of Streptomyces glaucescens tyrosinase. Replacement of His-37, -53, -193 or -215 by glutamine yields albino phenotypes, as determined by expression on melanin-indicator plates. The purified mutant proteins display no detectable oxy-enzyme and increased Cu lability at the binuclear active site.

Sex-dependent body weight changes after iontophoretic application of kainic acid into the LH or VMH.

Body weight changes and food and water intakes were studied in CFY male and female rats after kainic acid (KA)-induced destruction of the lateral hypothalamic area (LH) or the ventromedial hypothalamic nucleus (VMH). To minimize the extent of damages, KA was iontophoretically applied by means of glass micropipettes. KA was ejected in 50 or 80 mM concentrations with 5-15 microA current for 5 min.

Stimulation of histamine receptors of human monocytoid and hepatoma-derived cell lines and mouse hepatocytes modulates the production of the complement components C3, C4, factor B, and C2.

The influence of histamine (and the related agonists and antagonists) alone or in the presence of recombinant human interleukin 1 alpha (IL-1 alpha) and gamma interferon (IFN-gamma) was studied on the production of complement components C3, C2, factor B, and C4 in vitro with human monocytoid cell line U937, hepatoma-derived cell line HepG2, and mouse hepatocytes. Both U937 and HepG2 cells responded to histamine through H1 and H2 histamine receptors. The effect of histamine on the biosynthesis and gene expression of complement proteins was predominantly enhancing via the H1 histamine receptors and inhibitory through the H2 receptors.

Spreading of streptomycin antibiotic resistance gene in Escherichia coli plasmids demonstrated by Southern blot analysis.

Plasmids from E. coli strains of 38 donors were transconjugated to common recipient SY663 Escherichia coli K12. The restriction patterns of the isolated plasmids were highly heterogenous.

Lateral hypothalamic feeding mechanisms: iontophoretic effects of kainic acid, ibotenic acid and 6-hydroxydopamine.

In order to study hunger motivated behavior kainic acid (KA), ibotenic acid (IB) and 6-hydroxydopamine (6-OHDA) were iontophoretically applied to the lateral hypothalamus of rats. Neurotoxins at concentrations between 20-120 mMol were applied with 5-20 microA current for 5-10 min. Tip diameter of glass micropipettes varied between 10-50 micron.

Determination of phage type, colicin type and plasmid profile in Shigella sonnei.

Complex typing was carried out of 33 Shigella sonnei strains isolated in 1985 and 1986 One to six plasmids were carried by 32 strains typable by phages, whereas plasmid was not demonstrated in a phage-resistant strain. A 1.4 Md plasmid was carried by all strains.

Salmonella and shigella surveillance in Hungary, 1972-1976. II. Shigella surveillance.

Shigellae were isolated from 32 399 persons in the bacteriological laboratories of the Public Health Stations between 1972-1976. The number of isolations ranged between 5325 and 8237 yearly. As S.

A negative partition relation.

If the continuum hypothesis is assumed, there is a graph G whose vertices form an ordered set of type omega(1) (2); G does not contain triangles or complete even graphs of form [[unk](0),[unk](0)], and there is no independent subset of vertices of type omega(1) (2).