Effect of iron-based nanomaterials on organic carbon dynamics and greenhouse gas emissions during composting process.

Iron-based nanomaterials as effective additives can enhance the quality and safety of compost. However, their influence on organic carbon fractions changes and greenhouse gas emissions during composting remains unclear. This study demonstrated that iron-based nanomaterials facilitate the conversion of light organic carbon fraction into heavy organic carbon fraction, with the iron-based nanomaterials group showing a significantly higher heavy organic carbon fraction content (41.

Integrated transcriptome and H NMR-based metabolome to explore the potential mechanism of Spodoptera litura in response to flupyrimin.

Flupyrimin (FLP) is a novel class of insecticide acting on insect nicotinic acetylcholine receptor (nAChR) and shows robust insecticidal activity. However, the toxicological effects of FLP on Spodoptera litura have not been revealed. In this study, the results showed that the larval survival rate decreased significantly with increasing concentration of FLP.

A Combinatorial Single-Molecule Real-Time and Illumina Sequencing Analysis of Postembryonic Gene Expression in the Asian Citrus Psyllid .

Huanglongbing (HLB) is a systemic plant disease caused by ' Liberibacter asiaticus (Las)' and transmitted by . acquires the Las bacteria in the nymph stage and transmits it in the adult stage, indicating that molting from the nymph to adult stages is crucial for HLB transmission. However, the available reference genomes are incomplete, and gene function studies have been limited to date.

Synergistic effects of Fe-based nanomaterial catalyst on humic substances formation and microplastics mitigation during sewage sludge composting.

In this study, a novel Fe-based nanomaterial catalyst (Fe/FeS) was synthesized via a self-heating process and employed to explore its impact on the formation of humic substances and the mitigation of microplastics. The results reveal that Fe/FeS exhibited a significant increase in humic acid content (71.01 mg kg).

Natural Coumarin Shows Toxicity to by Inhibiting Detoxification Enzymes and Glycometabolism.

Coumarin and its derivatives are plant-derived compounds that exhibit potent insecticidal properties. In this study, we found that natural coumarin significantly inhibited the growth and development of larvae through toxicological assay. By transcriptomic sequencing, 80 and 45 differentially expressed genes (DEGs) related to detoxification were identified from 0 to 24 h and 24 to 48 h in after coumarin treatment, respectively.

Alternative splicing of chitin deacetylase 2 regulates chitin and fatty acid metabolism in Asian citrus psyllid, Diaphorina citri.

Chitin plays an important role in the development and molting of insects. The key genes involved in chitin metabolism were considered promising targets for pest control. In this study, two splice variants of chitin deacetylase 2 (CDA2) from Diaphorina citri were identified, including DcCDA2a and DcCDA2b.

New Insights into the Detoxifying Enzymes: Sequence Evolution, Structural Similarity, Functional Diversity, and Application Prospects of Glucosinolate Sulfatases.

Brassica plants have glucosinolate (GLs)-myrosinase defense mechanisms to deter herbivores. However, specifically feeds on Brassica vegetables. The larvae possess three glucosinolate sulfatases (PxGSS1-3) that compete with plant myrosinase for shared GLs substrates and produce nontoxic desulfo-GLs (deGLs).

Exploring the mechanisms of humic acid mediated degradation of polystyrene microplastics under ultraviolet light conditions.

Microplastics (MPs) are emerging pollutants that interact extensively with dissolved organic matter (DOM) and this influences the environmental behavior of MPs in aqueous ecosystems. However, the effect of DOM on the photodegradation of MPs in aqueous systems is still unclear. The photodegradation characteristics of polystyrene microplastics (PS-MPs) in an aqueous system in the presence of humic acid (HA, a signature compound of DOM) under ultraviolet light conditions were investigated in this study through Fourier transform infrared spectroscopy coupled with two-dimensional correlation analysis, electron paramagnetic resonance, and gas chromatography-mass spectrometry (GC/MS).

Sex-specific transcription and DNA methylation landscapes of the Asian citrus psyllid, a vector of huanglongbing pathogens.

The relationship of DNA methylation and sex-biased gene expression is of high interest, it allows research into mechanisms of sexual dimorphism and the development of potential novel strategies for insect pest control. The Asian citrus psyllid, Diaphorina citri Kuwayama, is a major vector for the causative agents of Huanglongbing (HLB), which presents an unparalleled challenge to citrus production worldwide. Here, we identify the X chromosome of D.

Single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification.

Plant cellulose microfibrils are increasingly employed to produce functional nanofibers and nanocrystals for biomaterials, but their catalytic formation and conversion mechanisms remain elusive. Here, we characterize length-reduced cellulose nanofibers assembly in situ accounting for the high density of amorphous cellulose regions in the natural rice fragile culm 16 (Osfc16) mutant defective in cellulose biosynthesis using both classic and advanced atomic force microscopy (AFM) techniques equipped with a single-molecular recognition system. By employing individual types of cellulases, we observe efficient enzymatic catalysis modes in the mutant, due to amorphous and inner-broken cellulose chains elevated as breakpoints for initiating and completing cellulose hydrolyses into higher-yield fermentable sugars.

Quantitative ubiquitylome crosstalk with proteome analysis revealed cytoskeleton proteins influence CLas pathogen infection in Diaphorina citri.

Huanglongbing (HLB), also known as citrus greening disease, is caused by Candidatus Liberbacter asiaticus (CLas) and transmitted by Diaphorina citri. Previous studies reported that CLas infection significantly influences the structure of the D. citri cytoskeleton.

Silencing of Significantly Inhibits Chitin and Fatty Acid Metabolism in Asian Citrus Psyllid, .

Glycogen is a predominant carbohydrate reserve in various organisms, which provides energy for different life activities. Glycogen synthase kinase 3 (GSK3) is a central player that catalyzes glucose and converts it into glycogen. In this study, a gene was identified from the genome database and named .

New insights into the distribution and interaction mechanism of microplastics with humic acid in river sediments.

Information on the distribution and interaction of microplastics (MPs) and humic acids (HAs) in river sediment has not been fully explored. This study assessed the distribution and interaction of MPs with HAs at different depths in river sediments. The results delineated that the average abundance of MPs in the 0-10 cm layer (190 ± 20 items/kg) was significantly lower than that in the 11-20 cm and 21-30 cm layers (211 ± 10 items/kg and 238 ± 18 items/kg, respectively).

Bombyx mori ferritin heavy-chain homolog facilitates BmNPV proliferation by inhibiting reactive oxygen species-mediated apoptosis.

Ferritin heavy-chain homolog (FerHCH), an iron-binding protein, plays an important role in the host defense against oxidative stress and pathogen infections. In our previous research, Bombyx mori native ferritin had an interaction with B. mori nucleopolyhedrovirus (BmNPV).

Silencing of Chitin-Binding Protein with PYPV-Rich Domain Impairs Cuticle and Wing Development in the Asian Citrus Psyllid, .

Chitin is a major component of the arthropod exoskeleton, always working together with chitin-binding proteins to maintain the functions of extracellular structures. In the present study, we identified a cuticle protein 64 from using a chitin-binding assay. Bioinformatics analysis revealed that DcCP64 contained eight conserved PYPV motifs but lacked a Rebers-Riddiford (R-R) consensus and other chitin-binding domains.

Insights into pectin dominated enhancements for elimination of toxic Cd and dye coupled with ethanol production in desirable lignocelluloses.

Pectin is a minor wall polysaccharide with potential applications for bioproducts. Despite the application of specific plants and biomass-based sorbents for environmental remediation, little has been reported about characteristic roles of pectin. Using the natural rice mutant (Osfc16) treated with Cd, this study explored that pectin could predominately enhance Cd accumulation with lignocellulose, mainly due to remarkably raised uronic acids deposition.

Functional analysis of a peptidoglycan recognition protein involved in the immune response in the common cutworm, Spodoptera litura.

Peptidoglycan recognition proteins (GRPs) are family of pattern recognition receptors (PRRs), which can recognize the peptidoglycan and trigger the innate immune system against the microorganisms in insects. In this study, we identified a GRP-LB from Spodoptera litura genome database and named SlGRP-LB, which contained a complete open reading frame (ORF) of 639 bp, encoding a protein of 212 amino acids with a signal peptide and GRP domain. Phylogenetic tree analysis suggested that the SlGRP-LB has a close relationship with Helicoverpa armigera GRP-LB (HaGRP-LB).

Down-regulation of OsMYB103L distinctively alters beta-1,4-glucan polymerization and cellulose microfibers assembly for enhanced biomass enzymatic saccharification in rice.

Background: As a major component of plant cell walls, cellulose provides the most abundant biomass resource convertible for biofuels. Since cellulose crystallinity and polymerization have been characterized as two major features accounting for lignocellulose recalcitrance against biomass enzymatic saccharification, genetic engineering of cellulose biosynthesis is increasingly considered as a promising solution in bioenergy crops. Although several transcription factors have been identified to regulate cellulose biosynthesis and plant cell wall formation, much remains unknown about its potential roles for genetic improvement of lignocellulose recalcitrance.

Functional Characterization of a Trehalose-6-Phosphate Synthase in Revealed by RNA Interference and Transcriptome Sequencing.

Trehalose-6-phosphate synthase (TPS) plays an important role in the synthesis of trehalose. In the current study, a gene was obtained from , and named as which encoded a protein of 833 amino acid residues. Real-time quantitative PCR (qPCR) analysis revealed that had the highest expression level in the midgut and fifth-instar nymph stage.

Validamycin treatment significantly inhibits the glycometabolism and chitin synthesis in the common cutworm, Spodoptera litura.

Validamycin, as a broadly applied antibiotic, has been used to control rice sheath blight disease. Furthermore, validamycin was considered as an insecticide to control agricultural pests. Insight into the mechanism of validamycin's action on insects can provide molecular targets for the control of agricultural pests.

An odorant-binding protein of Asian citrus psyllid, Diaphorina citri, participates in the response of host plant volatiles.

Background: Odorant-binding proteins (OBPs) in insects contribute to the sensitivity of the olfactory system and connect external odorants to olfactory receptor neurons. Determination of the chemosensory functions in Diaphorina citri, a vector of the citrus Huanglongbing pathogen, may help in developing a potential target for pest management.

Results: Diaphorina citri showed dose-dependent electroantennogram recording (EAG) responses to 12 host plant volatiles.

Integrated transcriptome sequencing and RNA interference reveals molecular changes in Diaphorina citri after exposure to validamycin.

Validamycin has been widely used as a specific competitive inhibitor of trehalase. In our previous research, validamycin significantly inhibited trehalase activity and chitin synthesis in Diaphorina citri, resulting in abnormal phenotypes. However, the mechanism of validamycin's action on D.

Liriopesides B from Liriope spicata var. prolifera inhibits metastasis and induces apoptosis in A2780 human ovarian cancer cells.

Ovarian cancer is the most frequent cause of death among gynecological cancers. In the present study, the anti‑cancer effect of liriopesides B, a steroidal saponin from Liriope spicata var. prolifera, against A2780 cells was investigated.