TEMI: Tissue Expansion Mass Spectrometry Imaging.

The spatial distribution of diverse biomolecules in multicellular organisms is essential for their physiological functions. High-throughput mapping of biomolecules is crucial for both basic and medical research, and requires high scanning speed, spatial resolution, and chemical sensitivity. Here, we developed a Tissue Expansion method compatible with matrix-assisted laser desorption/ionization Mass spectrometry Imaging (TEMI).

Expert consensus on the prevention and treatment of enamel demineralization in orthodontic treatment.

Enamel demineralization, the formation of white spot lesions, is a common issue in clinical orthodontic treatment. The appearance of white spot lesions not only affects the texture and health of dental hard tissues but also impacts the health and aesthetics of teeth after orthodontic treatment. The prevention, diagnosis, and treatment of white spot lesions that occur throughout the orthodontic treatment process involve multiple dental specialties.

High-Performance White Circularly Polarized Photoluminescence and Electroluminescence from Multi-Emission Enantiomers.

A pair of multi-emission enantiomers, (R)-DO-PSeZ and (S)-DO-PSeZ, was designed and synthesized by integrating Se-induced conformationally isomeric donors with a chiral carbonyl-containing acceptor into a unified molecular framework. The enantiomers showed white emission with blue, yellow and red multi-emission bands in solution and film at CIE coordinates of (0.39, 0.

Do market-based environmental regulations always promote enterprise green innovation commercialization?

Motivated by increasingly strengthened market-based environmental regulations, enterprises intend to pursue both pollution reduction and competitive edge through developing green innovation. Although the volume of green innovation has increased substantially, some of them are not successfully commercialized to pay rewards for enterprises. Hence, how market-based environmental regulations affect enterprise green innovation commercialization is urgently to be explored.

Spatial impact of environmental regulation on firm entry: Empirical evidence from Chinese firm registration data.

Facing severe environmental challenges and a rapidly changing economic landscape, it is important to deeply analyze the specific impact of environmental regulation on firm entry. This paper used data on enterprise registrations from 279 Chinese cities between 2007 and 2020. We employed spatial econometric models to study the spatial impact of environmental regulation on firm entry.

Establishment of Gas-Liquid-Solid Interface on Multilevel Porous CuO for Potential-Driven Selective CO Electroreduction toward C or C Products.

Copper-based catalysts demonstrate distinctive multicarbon product activity in the CO electroreduction reaction (CORR); however, their low selectivity presents significant challenges for practical applications. Herein, we have developed a multilevel porous spherical CuO structure, wherein the mesopores are enriched with catalytic active sites and effectively stabilize Cu, while the macropores facilitate the formation of a "gas-liquid-solid" three-phase interface, thereby creating a microenvironment with an increasing water concentration gradient from the interior to the exterior. Potential-driven phase engineering and protonation synergistically optimize the reaction pathway, facilitating a switch between CO and CH.

Flexible disk ultramicroelectrode: Facile preparation and high-resolution scanning electrochemical microscopy imaging.

Background: Scanning electrochemical microscopy (SECM) is a kind of scanning probe technology that enables the obtainment of surface morphology and electrochemical information by recording changes in Faraday current triggered by the movement of probe.

Results: In this work, flexible disk ultramicroelectrode (UME) with highly repeatable geometry are fabricated through a simple and universal strategy that involves vacuum pulling the glass capillaries inserted with platinum wire (gold wire, carbon fiber, etc.), followed by a rapidly heated sealing and polishing process.

Bacteria Derived from Diamondback Moth, (L.) (Lepidoptera: Plutellidae), Gut Regurgitant Negatively Regulate Glucose Oxidase-Mediated Anti-Defense Against Host Plant.

The ongoing interplay among plants, insects, and bacteria underscores the intricate balance of defense mechanisms in ecosystems. Regurgitant bacteria directly/indirectly impact plant immune responses, but the underlying mechanism is unclear. Here, we focus on the interaction between regurgitant bacteria, diamondback moth (DBM), and plant.

Systematic Review and Meta-Analysis of Application of Ultrasound-Guided Thoracic Paravertebral Block in Clinical Surgical Treatment.

Aim: There is a lack of consensus regarding the efficacy of thoracic paravertebral block (TPVB) and erector spinae plane block (ESPB) for postoperative pain in randomized controlled trials (RCTs). The comparison of TPVB and ESPB was explored through a systematic review and meta-analysis (MA) of relevant RCTs.

Methods: A comprehensive search of relevant literature was conducted using databases such as PubMed, Embase, and MEDLINE, from 2019 to June 2024.

Study on the Mechanism of Bifidobacterium animalis subsp. lactis F1-3-2 Regulating Bile Acid Metabolism Through TMA-TMAO Pathway to Improve Atherosclerosis.

Atherosclerosis is a major cause of cardiovascular disease (CVD). The trimethylamine (TMA)-trimethylamine N-oxide (TMAO) pathway is a key crossover pathway highly associated with diet, gut microbiome, and atherosclerosis. The Bifidobacterium animalis subsp.

Particulate hexavalent chromium exposure induces DNA double-strand breaks and inhibits homologous recombination repair in rat and human lung tissues.

Lung cancer is an important human health concern because of its high mortality rate, with many cases caused by environmental chemicals other than tobacco. Particulate hexavalent chromium [Cr(VI)] is a well-established human lung carcinogen, but how Cr(VI) induces lung cancer is poorly understood. Chromosome instability, a hallmark of lung cancer, is considered a major driving factor in Cr(VI)-induced lung cancer.

Meta-Analysis of Transperineal and Transrectal Ultrasound-Guided Prostate Biopsy in the Detection of Prostate Cancer.

Background: Transperineal (TP) biopsy is increasingly used as an alternative to standard transrectal (TR) biopsy for prostate cancer detection to reduce infection risks. However, evidence on comparative diagnostic accuracy remains inconclusive. The aim of this study was to perform an updated systematic review and meta-analysis of studies comparing prostate cancer detection rates between TP and TR ultrasound biopsies.

Effects of Mandibular Advancement Device on Genioglossus of Rabbits in Obstructive Sleep Apnea Through PINK1/Parkin Pathway.

Background: Early treatment of mandibular advancement device (MAD) reverses the abnormal changes resulting from obstructive sleep apnoea (OSA), but the underlying mechanism is not clear. We analysed the changes of genioglossus function before and after MAD treatment in OSA rabbits and explored the mechanism of mitochondrial autophagy.

Methods: Eighteen male New Zealand rabbits were randomised into three groups: the control group, Group OSA, and Group MAD.

Strategic dynamics of local governments in regional collaborative governance: An evolutionary game theory analysis of haze pollution response in the Fen-Wei Plain, China.

Regional collaborative governance has become a key strategy for environmental protection, especially in reducing transboundary pollution transfer. This study, set against the backdrop of environmental governance in China's Fen-Wei Plain, employs evolutionary game theory to deeply analyze the strategic choices of local governments in managing haze pollution. We developed a model incorporating 14 key variables to systematically explore the emission reduction strategies of local governments under various policy environments.

EGCG protects intestines of mice and pelvic cancer patients against radiation injury via the gut microbiota/D-tagatose/AMPK axis.

Background And Purpose: Radiation-induced intestinal injury (RIII) compromises the clinical utility of pelvic radiotherapy (RT). We aimed to explore the protective effect and underlying mechanism of (-)-epigallocatechin-3-gallate (EGCG) on RIII.

Materials And Methods: We evaluated the protective effect of EGCG on intestine in RIII mouse model and pelvic cancer patients, while explored the underlying mechanism through (1) 16S rRNA sequencing, (2) metabolomic profiles, (3) fresh sterile fecal filtrate (SFF) transplantation, and (4) transcriptome sequencing.

Carcinogenic Mechanisms of Hexavalent Chromium: From DNA Breaks to Chromosome Instability and Neoplastic Transformation.

Purpose Of Review: Hexavalent chromium [Cr(VI)] is a well-established human carcinogen, yet the mechanisms by which it leads to carcinogenic outcomes is still unclear. As a driving factor in its carcinogenic mechanism, Cr(VI) causes DNA double strand breaks and break-repair deficiency, leading to the development of chromosome instability. Therefore, the aim of this review is to discuss studies assessing Cr(VI)-induced DNA double strand breaks, chromosome damage and instability, and neoplastic transformation including cell culture, experimental animal, human pathology and epidemiology studies.

Screening and identification of linear B-cell epitopes on structural proteins of African Swine Fever Virus.

This study aims to screen and identify linear B-cell epitopes on the structural proteins of African Swine Fever Virus (ASFV) to assist in the development of peptide-based vaccines. In experiments, 66 peptides of 12 structural proteins of ASFV were predicted as potential linear B-cell epitopes using bioinformatics tools and were designed; the potential epitope proteins carried the GST tag were expressed, purified, and subjected to antigenicity analysis with porcine antiserum against ASFV, and further identified based on their immunogenicity in mice. A total of 22 potential linear B-cell epitopes showed immunoreactivity and immunogenicity.

Mg-Doped Cu Catalyst for Electroreduction of CO to Multicarbon Products: Lewis Acid Sites Simultaneously Promote *CO Adsorption and Water Dissociation.

The electroreduction of CO to valuable fuels or high-value chemicals by using sustainable electric energy provides a promising strategy for solving environmental problems dominated by the greenhouse effect. Copper-based materials are the only catalysts that can convert CO into multicarbon products, but they are plagued by high potential, low selectivity, and poor stability. The key factors to optimize the conversion of CO into multicarbon products are to improve the adsorption capacity of intermediates on the catalyst surface, accelerate the hydrogenation step, and improve the C-C coupling efficiency.

Comparative study of ten machine learning algorithms for short-term forecasting in gas warning systems.

This research aims to explore more efficient machine learning (ML) algorithms with better performance for short-term forecasting. Up-to-date literature shows a lack of research on selecting practical ML algorithms for short-term forecasting in real-time industrial applications. This research uses a quantitative and qualitative mixed method combining two rounds of literature reviews, a case study, and a comparative analysis.

Mass Spectrometry Structural Proteomics Enabled by Limited Proteolysis and Cross-Linking.

The exploration of protein structure and function stands at the forefront of life science and represents an ever-expanding focus in the development of proteomics. As mass spectrometry (MS) offers readout of protein conformational changes at both the protein and peptide levels, MS-based structural proteomics is making significant strides in the realms of structural and molecular biology, complementing traditional structural biology techniques. This review focuses on two powerful MS-based techniques for peptide-level readout, namely limited proteolysis-mass spectrometry (LiP-MS) and cross-linking mass spectrometry (XL-MS).

Mass spectrometry imaging for spatially resolved multi-omics molecular mapping.

The recent upswing in the integration of spatial multi-omics for conducting multidimensional information measurements is opening a new chapter in biological research. Mapping the landscape of various biomolecules including metabolites, proteins, nucleic acids, etc., and even deciphering their functional interactions and pathways is believed to provide a more holistic and nuanced exploration of the molecular intricacies within living systems.

Enhancing local K adsorption by high-density cube corners for efficient electroreduction of CO to C products.

Reducing carbon dioxide (CO) to high value-added chemicals using renewable electricity is a promising approach to reducing CO levels in the air and mitigating the greenhouse effect, which depends on high-efficiency electrocatalysts. Copper-based catalysts can be used for electroreduction of CO to produce C products with high added value, but suffer from poor stability and low selectivity. Herein, we propose a strategy to enhance the field effect by varying the cubic corner density on the surface of CuO microspheres for improving the electrocatalytic performance of CO reduction to C products.