Retrospective Study on the Value of Intensive Care Unit Noise Reduction Management in Elderly Patients with Chronic Renal Failure in the Uremic Phase.

Objective: This study aimed to investigate the value of noise reduction management in the intensive care unit (ICU) in elderly patients with chronic renal failure (CRF) in the uremic phase.

Methods: A retrospective study was conducted, including 150 elderly patients with CRF in the uremic phase, who were treated in the ICU ward at the First Affiliated Hospital of Soochow University between January 2021 and August 2023. Among them, 73 were in the control group (routine ICU management), and 77 were in the observation group (routine ICU management + ICU noise reduction management).

Development and evaluation of a CRISPR-Cas13a system-based diagnostic for hepatitis E virus.

Objectives: Hepatitis E virus (HEV) is the leading cause of acute viral hepatitis worldwide. HEV RNA detection is the gold standard for HEV infection diagnosis and PCR methods are commonly used but are usually time-consuming and expensive, resulting in low detection efficiency and coverage, especially in low-income areas. Here, we developed a simpler and more accessible HEV RNA detection method based on CRISPR-Cas13a system.

Construction of a hepatocellular carcinoma high-risk population rating scale and independent predictors' assessment.

Background: With increasing mortality and incidence, hepatocellular carcinoma (HCC) has become a major public health problem. The early diagnosis of HCC can improve its prognosis. The aim of this study was to identify potential risk factors related to HCC development and to establish a high-risk population rating scale.

Establishment and Characterization of the First Chinese National Standard for Nucleic Acid Amplification Technology Assays for Hepatitis E Virus Nucleic Acid Detection.

The detection of hepatitis E virus (HEV) RNA is the gold standard for HEV infection diagnosis. In order to address the quality control requirements for HEV RNA detection kits within China, we aimed to establish the first Chinese national standard for HEV RNA detection through a collaborative study. The candidate standard was quantified using digital PCR (dPCR).

Improving the effectiveness of watershed environmental management-dynamic coordination through government pollution control and resident participation.

To improve the effectiveness of envir onmental management of watersheds and improve the environmental management mechanism of cross-administrative watersheds, we develop a neoliberal framework for action using incentives, examine the cooperative strategies of local governments in watershed treatment and people-oriented environmental protection under central government subsidies, and analyze the cost effectiveness of multiple strategies in a dynamic perspective, and we have the following important findings: (1) Compared to vertical ecological compensation, the introduction of horizontal cost-sharing contracts is more effective in enhancing inter-local cooperative environmental governance. (2) When the marginal benefit of the downstream local government is greater than half of the upstream marginal benefit, the upstream local government's pollution control investment and the effect of pollution control are improved, and the Pareto improvement of the environmental governance benefit of the watershed is realized, i.e.

DeepGpgs: a novel deep learning framework for predicting arginine methylation sites combined with Gaussian prior and gated self-attention mechanism.

Protein arginine methylation is an important posttranslational modification (PTM) associated with protein functional diversity and pathological conditions including cancer. Identification of methylation binding sites facilitates a better understanding of the molecular function of proteins. Recent developments in the field of deep neural networks have led to a proliferation of deep learning-based methylation identification studies because of their fast and accurate prediction.

Does Water Matter? The Impact of Water Vulnerability on Corporate Financial Performance.

This study aims to understand the potential relationship between water vulnerability and corporate financial performance for listed companies in China. Studies have argued that water risk has begun to affect the sustainability of firms, but few studies have included water conditions in the research framework to examine whether and how water conditions have a direct impact on firms. In addition, studies on environment governance have emphasized the impact of government environmental regulation on firms.

Evaluation of factors contributing to variability of qualitative and quantitative proficiency testing for SARS-CoV-2 nucleic acid detection.

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to unprecedented social and economic disruption. Many nucleic acid testing (NAT) laboratories in China have been established to control the epidemic better. This proficiency testing (PT) aims to evaluate the participants' performance in qualitative and quantitative SARS-CoV-2 NAT and to explore the factors that contribute to differences in detection capabilities.

Economic benefit evaluation of water resources allocation in transboundary basins based on particle swarm optimization algorithm and cooperative game model-A case study of Lancang-Mekong River Basin.

The present work aims to find the optimal solution of Nash Equilibrium (NE) in the traditional Game Theory (GT) applied to water resources allocation. Innovatively, this paper introduces Particle Swarm Optimization (PSO) into GT to propose a cooperative game model to solve the NE problem. Firstly, the basic theory of the PSO algorithm and cooperative game model is described.

Argonaute-integrated isothermal amplification for rapid, portable, multiplex detection of SARS-CoV-2 and influenza viruses.

Isothermal amplification methods are a promising trend in virus detection because of their superiority in rapidity and sensitivity. However, the generation of false positives and limited multiplexity are major bottlenecks that must be addressed. In this study, we developed a multiplex Argonaute (Ago)-based nucleic acid detection system (MULAN) that integrates rapid isothermal amplification with the multiplex inclusiveness of a single Ago for simultaneous detection of multiple targets such as SARS-CoV-2 and influenza viruses.

Multicenter assessment of shotgun metagenomics for pathogen detection.

Background: Shotgun metagenomics has been used clinically for diagnosing infectious diseases. However, most technical assessments have been limited to individual sets of reference standards, experimental workflows, and laboratories.

Methods: A reference panel and performance metrics were designed and used to examine the performance of shotgun metagenomics at 17 laboratories in a coordinated collaborative study.

Computed Tomography Image Texture under Feature Extraction Algorithm in the Diagnosis of Effect of Specific Nursing Intervention on Mycoplasma Pneumonia in Children.

To evaluate the effect of specific nursing intervention in children with mycoplasma pneumonia (MP), a feature extraction algorithm based on gray level co-occurrence matrix (GLCM) was proposed and combined with computed tomography (CT) image texture features. Then, 98 children with MP were rolled into the observation group with 49 cases (specific nursing) and the control group with 49 cases (routine nursing). CT images based on feature extraction algorithm of optimized GLCM were used to examine the children before and after nursing intervention, and the recovery of the two groups of children was discussed.

The impact of sample processing on the rapid antigen detection test for SARS-CoV-2: Virus inactivation, VTM selection, and sample preservation.

Many factors have been identified as having the ability to affect the sensitivity of rapid antigen detection (RAD) tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This study aimed to identify the impact of sample processing on the sensitivity of the RAD tests. We explored the effect of different inactivation methods, viral transport media (VTM) solutions, and sample preservation on the sensitivity of four RAD kits based on two SARS-CoV-2 strains.

Characteristics of enhanced biological phosphorus removal (EBPR) process under the combined actions of intracellular and extracellular polyphosphate.

The characteristics of enhanced biological phosphorus removal (EBPR) process under the combined actions of intracellular and extracellular polyphosphate (polyP) were investigated with the P Nuclear Magnetic Resonance (NMR) and the fractionation extracting the loosely-bound and tightly-bound extracellular polymer substances (i.e., LB-EPS and TB-EPS) and bacterial cells in EBPR sludge.

A SARS-CoV-2 Reference Standard Quantified by Multiple Digital PCR Platforms for Quality Assessment of Molecular Tests.

The outbreak of novel coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide. To meet the urgent and massive demand for the screening and diagnosis of infected individuals, many in vitro diagnostic assays using nucleic acid tests (NATs) have been urgently authorized by regulators worldwide. A reference standard with a well-characterized concentration or titer is of the utmost importance for the study of limit of detection (LoD), which is a crucial feature for a diagnostic assay.

Weight prioritized slicing based on constraint logic programming for fault localization.

Fault localization, a technique to fix and ensure the dependability of software, is rapidly becoming infeasible due to the increasing scale and complexity of multilingual programs. Compared to other fault localization techniques, slicing can directly narrow the range of the code which needed checking by abstracting a program into a reduced one by deleting irrelevant parts. Only minority slicing methods take into account the fact that the probability of different statements leading to failure is different.

Correlation between expression of Ki-67 and MSCT signs in different types of lung adenocarcinoma.

To investigate the correlation between the proliferating cell nuclear antigen Ki-67 and the multislice computed tomography (MSCT) signs in different subtypes of lung adenocarcinoma.Ninety-five patients with lung adenocarcinoma confirmed by surgical pathology and treated between January 2017 and December 2017 were included. MSCT was performed before the operation, and the characteristics of the high-resolution CT (HRCT) signs of the lesions were compared with the Ki-67 immunohistochemistry results.

HIV-1 low-level viremia affects T cell activation rather than T cell development in school-age children, adolescents, and young adults during antiretroviral therapy.

Objectives: Given the improvements in antiretroviral therapy (ART) in recent years, more pediatric HIV patients receiving ART are reaching adolescence and adulthood. This study investigated the influence of poor virological response (low-level viremia (LLV) and virological failure (VF)) on the immune system of these patients.

Methods: HIV-infected, ART-experienced pediatric patients (n=206) were enrolled in this cross-sectional study.

Multiplex Cell-Free DNA Reference Materials for Quality Control of Next-Generation Sequencing-Based Diagnostic Tests of Colorectal Cancer Tolerance.

Liquid biopsies based on next-generation sequencing (NGS) assays are confronted with more opportunities and challenges. Widespread clinical implementation of NGS-based cancer diagnostic tests (IVDs) highlighted the urgency to establish reference materials (RMs) which could provide full control of the process from nucleic acid extraction to test report generation. Quality control based on cell-free DNA (cfDNA) RMs is especially important for liquid biopsies.

Quality Control of Next-generation Sequencing-based Diagnostic Test for Onco-relevant Mutations Using Multiplex Reference Materials in Plasma.

Widespread clinical implementation of next-generation sequencing (NGS)-based cancer diagnostic tests (IVDs) highlighted the urgency to establish reference materials which could provide full control of the process from nucleic acid extraction to test report generation. The formalin-fixed, paraffin-embedded (FFPE) tissue and blood plasma containing circulating tumor deoxyribonucleic acid (ctDNA) were mostly used for clinically detecting onco-relevant mutations. We respectively developed multiplex FFPE and plasma reference materials covering three clinically onco-relevant mutations within the () gene at serial allelic frequencies.

Evaluation of Neoseiulus barkeri (Acari: Phytoseiidae) for Control of Eotetranychus kankitus (Acari: Tetranychidae).

Eotetranychus kankitus Ehara (Acari: Tetranychidae) is an important pest in Chinese citrus orchards. In the present study, we aimed to evaluate the potential of Neoseiulus barkeri (Hughes) (Acari: Phytoseiidae) for the biological control of E. kankitus.

Evolution, opportunity and challenges of transboundary water and energy problems in Central Asia.

Central Asia is one of the regions that suffer the most prominent transboundary water and energy problems in the world. Effective transboundary water-energy resource management and cooperation are closely related with socioeconomic development and stability in the entire Central Asia. Similar to Central Asia, Northwest China has an arid climate and is experiencing a water shortage.

[Study on Spatial Dispersal and Migration Events of Japanese Encephalitis Virus].

To explore the spatial distribution mechanism of Japanese encephalitis virus (JEV), PhyML v3.0 was used to build phylogenetic tree using JEV sequences in the dataset. PAUP v4.

Low prevalence of human leukocyte antigen-B*5701 in HIV-1-infected Chinese subjects: a prospective epidemiological investigation.

Background: Human leukocyte antigen (HLA)-B*5701 is strongly associated with developing a hypersensitivity reaction to abacavir (ABC). Limited data exist on HLA-B*5701 prevalence in HIV-1-infected subjects in China. We investigated HLA-B*5701 prevalence in HIV-1-infected population including Han and non-Han ethnic groups.